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CD3D & CD3E Recombinant Mouse Protein, hIgG1-Fc & hIgG1-Fc.His tag

Catalog number: CT025M2303H25

 Related applications: Protein Assays and Analysis

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Description

CD3 Antigen delta and CD3 Antigen epsilon Heterodimer (CD3D & CD3E), recombinant mouse protein is supplied as a lyophilized powder.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Ala 105) of mouse CD3D (P04235) fused to the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; a DNA sequence encoding the extracellular domain (Met 1-Asp 108) of mouse CD3E (P22646) fused to the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD3D/CD3E heterodimer was purified.

Activity: This recombinant protein has not been tested.

Formulation: 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by SINO Biological.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: CD Proteins
Research Category: Immunology, Signal Transduction
Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: None
Gene ID: 12500, 12501
Gene Symbol: CD3D & CD3E
Molecular Weight: 76 kDa
Product Line: Sino Biological®
Product Size: 5 x 5 µg
Protein Form: Recombinant, Truncated
Protein Name: CD3 Antigen delta and CD3 Antigen epsilon Heterodimer
Protein Tag or Fusion: DYKDDDDK, His Tag, IgG1 Fc Tag
Purification: PAGE
Purity: ≥ 85% by SDS-PAGE
Species: Mouse
Tag Position: C-Terminal
Validated Application: Assay Standard

Contents & storage

Includes 5 vials of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols