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CD3D & CD3E Recombinant Mouse Protein, hIgG1-Fc & hIgG1-Fc.His tag

Catalog number: CT025M2303H50

 Related applications: Protein Assays and Analysis

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CD3 Antigen delta and CD3 Antigen epsilon Heterodimer (CD3D & CD3E), recombinant mouse protein is supplied as a lyophilized powder.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Ala 105) of mouse CD3D (P04235) fused to the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; a DNA sequence encoding the extracellular domain (Met 1-Asp 108) of mouse CD3E (P22646) fused to the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD3D/CD3E heterodimer was purified.

Activity: This recombinant protein has not been tested.

Formulation: 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by SINO Biological.

For Research Use Only. Not for use in diagnostic procedures.


Form: Lyophilized
Purity: ≥ 85% by SDS-PAGE
Gene ID: 12500, 12501
Species: Mouse
Expression: Mammalian
Gene Symbol: CD3D & CD3E
Gene Aliases: None
Product Size: 50 µg
Protein Form: Truncated, Recombinant
Purification: PAGE
Tag Position: C-Terminal
Protein Family: CD Proteins
Endotoxin Level: < 1 EU/µg
Molecular Weight: 76 kDa
Research Category: Immunology, Signal Transduction
Protein Tag or Fusion: His Tag, IgG1 Fc Tag, DYKDDDDK
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.


Manuals & protocols