Identification of the C-terminal part of Bordetella dermonecrotic toxin as a transglutaminase for rho GTPases.
AuthorsSchmidt G,Goehring UM,Schirmer J,Lerm M,Aktories K
JournalThe Journal of biological chemistry
PubMed ID10542213
The lysosomotropic agent monodansylcadaverine also acts as a solvent polarity probe.
AuthorsNiemann A,Takatsuki A,Elsässer HP
JournalThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
PubMed ID10639491
The role of cross-linking in epidermal differentiation.
AuthorsKubilus J, Baden HP
JournalCurr Probl Dermatol
PubMed ID6197245
Cultured bovine keratinocytes contain two major classes of transglutaminase substrate proteins. These may be separated on Sephadex G-75 into a 30,000-to-50,000-dalton fraction and a fraction which is heavier than 75,000 daltons. By SDS-electrophoresis, the lighter fraction consists of two components of molecular weights 9,000 and 18,000 daltons, while the heavier ... More
Transglutaminase-catalyzed cross-linking of fibrils of collagen V/XI in A204 rhabdomyosarcoma cells.
AuthorsKleman JP, Aeschlimann D, Paulsson M, van der Rest M
JournalBiochemistry
PubMed ID7577969
'Collagens V and XI are thought to form a core around which the major interstitial collagens, I and II, respectively, are organized during fibrillogenesis. We previously reported the presence of a heterotypic form of collagens V and XI, [alpha 1(XI)]2 alpha 2(V), in cultures of A204 rhabdomyosarcoma cells [Kleman, J.-P., ... More
Identification of transmembrane bridging proteins in the plasma membrane of cultured mouse L cells.
AuthorsEvans RM, Fink LM
JournalProc Natl Acad Sci U S A
PubMed ID23534
'Studies were carried out to identify transmembrane bridging proteins in the plasma membrane of mouse L-929 cells. Cells grown in suspension culture were 125I-labeled by lactoperoxidase and allowed to ingest latex particles to produce inside-out membrane phagosome preparations. Phagosomes were isolated and the inner membrane surface was labeled with N-(5''-aminopentyl)-5-dimethylamino-1-naphthalenesulfonamide ... More
Trafficking patterns of beta-arrestin and G protein-coupled receptors determined by the kinetics of beta-arrestin deubiquitination.
AuthorsShenoy SK, Lefkowitz RJ
JournalJ Biol Chem
PubMed ID12574160
'Agonist-dependent internalization of G protein-coupled receptors via clathrin-coated pits is dependent on the adaptor protein beta-arrestin, which interacts with elements of the endocytic machinery such as AP2 and clathrin. For the beta(2)-adrenergic receptor (beta(2)AR) this requires ubiquitination of beta-arrestin by E3 ubiquitin ligase, Mdm2. Based on trafficking patterns and affinity ... More
Pharmacology and functional properties of NTS2 neurotensin receptors in cerebellar granule cells.
AuthorsSarret P, Gendron L, Kilian P, Nguyen HM, Gallo-Payet N, Payet MD, Beaudet A
JournalJ Biol Chem
PubMed ID12084713
'The binding and signaling properties of neuronal NTS2 neurotensin (NT) receptors were examined in cultured rat cerebellar granule cells. As shown by reverse transcription-PCR, receptor autoradiography, and confocal microscopic localization of fluorescent NT, these cells selectively express the NTS2 receptor subtype. Accordingly, a single apparent class of (125)I-NT-binding sites, with ... More
Role of the secretion vesicle in the transport of receptors: modulation of somatostatin binding to pancreatic islets.
AuthorsSussman KE, Mehler PS, Leitner JW, Draznin B
JournalEndocrinology
PubMed ID6123431
Two pathways for insulin metabolism in adipocytes.
AuthorsDuckworth WC, Hamel FG, Peavy DE
JournalBiochim Biophys Acta
PubMed ID9332452
'Using selected conditions, the appropriate collagenase, albumin and cell treatment, a preparation of isolated adipocytes was developed with no extracellular insulin degrading activity. Cell mediated insulin degradation rates were 0.68% +/- 0.05%/100,000 cell/h using trichloracetic acid precipitability as a measure. Chloroquine (CQ) increased cell-associated radioactivity and decreased degradation while dansylcadaverine ... More
Expression of haptoglobin receptors in human hepatoma cells.
AuthorsOkuda M, Tokunaga R, Taketani S
JournalBiochim Biophys Acta
PubMed ID1354488
'The uptake of radio-labeled hemoglobin-haptoglobin complex (Hb-Hp) by human hepatoma PLC/PRF/5 and HepG2 cells was investigated in an attempt to characterize the uptake process and intracellular transport. Human hepatoma cells took up Hb-Hp in a receptor-mediated manner. Scatchard analysis of binding revealed that PLC/PRF/5 and HepG2 cells exhibited about 21,000 ... More
Involvement of the vacuolar H(+)-ATPase in animal virus entry.
AuthorsPérez L, Carrasco L
JournalJ Gen Virol
PubMed ID7931146
'Semliki Forest virus (SFV) enters cells by receptor-mediated endocytosis, followed by acidification of endosomes by the action of the vacuolar H(+)-ATPase. Fusion of the viral and the endosomal membrane delivers the viral genome to the cytoplasm. Direct blockade of the vacuolar H(+)-ATPase by the selective inhibitor bafilomycin A1 (BFLA1) prevented ... More
Invasion of cultured human epithelial cells by Klebsiella pneumoniae isolated from the urinary tract.
AuthorsOelschlaeger TA, Tall BD
JournalInfect Immun
PubMed ID9199471
'The mechanisms which enable entry into cultured human epithelial cells by Klebsiella pneumoniae were compared with those of Salmonella typhi Ty2. K. pneumoniae 3091, isolated from a urine sample of a patient with a urinary tract infection, invaded human epithelial cells from the bladder and ileocecum and persisted for days ... More
Involvement of membrane-bound transglutaminase in the invagination of transferrin into rat reticulocyte plasma membrane.
AuthorsKatoh S, Midorikami J, Takasu S, Ohkubo Y
JournalBiol Pharm Bull
PubMed ID7820097
'We demonstrated the invagination of transferrin into reticulocyte plasma membrane to learn whether membrane-bound transglutaminase (TGase, a Ca(2+)-dependent enzyme) is involved in this invagination. The invagination was assessed by acid-resistance assay and antibody-inaccessibility assay. The invagination was blocked in the absence of ATP. [14C]Putrescine, a substrate for TGase, was incorporated ... More
Modification of fibroblast surface amines alters receptor-mediated cell spreading on protein-coated substrata but not adsorptive endocytosis.
AuthorsAplin JD, Hughes RC
JournalJ Cell Sci
PubMed ID6118378
'Fluorescein isothiocyanate (FITC) and other anionic reagents specific for amine groups have previously been shown to inhibit the adhesion and spreading of cultured fibroblasts to fibronectin-coated surfaces (Butters, Devalia, Aplin & Hughes, 1980). Here it is demonstrated that a population of FITC-labelled cells can be separated using flow cytometry into ... More
The entry of African swine fever virus into Vero cells.
AuthorsAlcamí A, Carrascosa AL, Viñuela E
JournalVirology
PubMed ID2741349
'The entry of African swine fever virus into Vero cells has been investigated by both biochemical and morphological techniques. A quantitative electron microscopy analysis of the early steps of the infection has shown that African swine fever virus enters Vero cells by a receptor-mediated endocytosis mechanism. The internalization of virus ... More
Human plasma fibronectin structure probed by steady-state fluorescence polarization: evidence for a rigid oblate structure.
'In order to more clearly define the structure of human plasma fibronectin (PFn) under physiologic buffer conditions, we determined the mean harmonic rotational relaxation times (rho H) of PFn and the thrombin-derived 190/170-kDa PFn fragment using steady-state fluorescence polarization. These measurements utilized the long lifetime emission (tau = 1.2 X ... More
Semenogelin I and semenogelin II, the major gel-forming proteins in human semen, are substrates for transglutaminase.
AuthorsPeter A, Lilja H, Lundwall A, Malm J
JournalEur J Biochem
PubMed ID9523691
'The major seminal vesicle secreted proteins in human semen, semenogelin I and semenogelin II, interact non-covalently and via disulphide bridges to instantly form a coagulum upon ejaculation. The coagulum is liquefied after a few minutes due to the action of a prostatic serine protease, prostate-specific antigen (PSA). In contrast to ... More
Calmodulin dependence of transferrin receptor recycling in rat reticulocytes.
AuthorsGrasso JA, Bruno M, Yates AA, Wei LT, Epstein PM
JournalBiochem J
PubMed ID2310376
'Kinetic analysis of transferrin receptor properties in 6-8 day rat reticulocytes showed the existence of a single class of high-affinity receptors (Kd 3-10 nM), of which 20-25% were located at the cell surface and the remainder within an intracellular pool. Total transferrin receptor cycling time was 3.9 min. These studies ... More
Cell association and invasion of Caco-2 cells by Campylobacter jejuni.
AuthorsRussell RG, Blake DC
JournalInfect Immun
PubMed ID8063393
'Adherence and invasion studies were conducted in monolayers of Caco-2 cells. Three-day-old monolayers were inoculated with Campylobacter jejuni 81-176 at a bacterium/cell ratio of 1,000:1. Saturation studies demonstrated time- and dose-dependent saturation curves for C. jejuni cell association and invasion into Caco-2 cells. Electron microscopy revealed intracellular C. jejuni located ... More
pH-independent retrograde targeting of glycolipids to the Golgi complex.
AuthorsSchapiro FB, Lingwood C, Furuya W, Grinstein S
JournalAm J Physiol
PubMed ID9486120
'A small fraction of the molecules internalized by endocytosis reaches the Golgi complex through a retrograde pathway that is poorly understood. In the present work, we used bacterial toxins to study the retrograde pathway in Vero cells. The recombinant B subunit of verotoxin 1B (VT1B) was labeled with fluorescein to ... More
Uptake of thyroxine in cultured anterior pituitary cells of euthyroid rats.
AuthorsEverts ME, Docter R, Moerings EP, van Koetsveld PM, Visser TJ, de Jong M, Krenning EP, Hennemann G
JournalEndocrinology
PubMed ID8194475
'The uptake of [125I]T4 was investigated in cultured anterior pituitary cells isolated from adult fed Wistar rats and cultured for 3 days in medium containing 10% fetal calf serum. Experiments were performed with [125I]T4 (10(5) to 2 x 10(6) cpm; 0.35-7 nM) in medium containing 0.5% or 0.1% BSA. The ... More
Effect of endocytic and metabolic inhibitors on the internalization and intracellular growth of Brucella abortus in Vero cells.
AuthorsDetilleux PG, Deyoe BL, Cheville NF
JournalAm J Vet Res
PubMed ID1767988
'Uptake, transfer to rough endoplasmic reticulum, and intracellular growth of Brucella abortus were studied in Vero cells treated with endocytic and metabolic inhibitors. Infection of Vero cells was suppressed when inhibitors of energy metabolism (iodoacetate, dinitrophenol), receptor-mediated endocytosis (monodansylcadaverine, amantadine, methylamine), or endosomal acidification (chloroquine, ammonium chloride, monensin) were added ... More
Inhibition of pokeweed mitogen-induced B cell differentiation by compounds containing primary amine or hydrazine groups.
Authorsde Boccardo G, Drayer D, Rubin AL, Novogrodsky A, Reidenberg MM, Stenzel KH
JournalClin Exp Immunol
PubMed ID3882287
'The present study examined the effect of two drugs, which contain either an aromatic amine or hydrazine moiety and are known to induce lupus like syndromes in man (procainamide and hydralazine) and an aliphatic amine (dansylcadaverine), on pokeweed mitogen (PWM)-induced B cell production of immunoglobulin G (IgG). These compounds all ... More
Degradation of insulin receptors by hepatoma cells: insulin-induced down-regulation results from an increase in the rate of basal receptor degradation.
'The degradation of insulin receptors was studied in cultured Zajdela hepatoma cells (ZHC). Receptor distribution within the cell was evaluated by estimating: i) surface receptor level on entire cells, ii) total cell receptors solubilized by Triton from cell membranes and iii) intracellular receptors solubilized from cells whose surface receptors had ... More
Calcium pretreatment induces the decrease in epidermal growth factor binding through the activation of transglutaminase in isolated liver membrane.
AuthorsKatoh S, Hashimoto M, Kohno H, Ohkubo Y
JournalArch Biochem Biophys
PubMed ID8099784
'Pretreatment of hepatocytes with Ca2+ and A23187 resulted in the decrease in epidermal growth factor (EGF) binding to the receptor. This decrease could be induced by only the pretreatment of the isolated liver membrane with Ca2+. By the pretreatment with 1 mM Ca2+ for 30 min, EGF binding was decreased ... More
Dansylcadaverine inhibits internalization of 125I-epidermal growth factor in BALB 3T3 cells.
AuthorsHaigler HT, Maxfield FR, Willingham MC, Pastan I
JournalJ Biol Chem
PubMed ID6243633
'The binding and internalization of 125I-epidermal growth factor (125I-EGF) was studied in cultures of BALB 3T3 cells using a novel method that involved removal of cell-surface hormone by treatment with acetic acid under conditions that did not remove internalized hormone. In control cultures, 125I-EGF initially bound to its receptor on ... More
Effects of lysosomotropic amines on human polymorphonuclear leucocyte function.
AuthorsVandenbroucke-Grauls CM, Thijssen RM, Marcelis JH, Sharma SD, Verhoef J
JournalImmunology
PubMed ID6319273
'Lysosomotropic agents interfere with lysosome function. We studied the effects of the lysosomotropic amines: lidocaine, diphenylamine and dansylcadaverine on several functions of human polymorphonuclear leucocytes (PMN): enzyme release, phagosome-lysosome fusion, superoxide anion generation upon stimulation with opsonized bacteria, and phagocytosis and killing of opsonized Staphylococcus aureus. Lidocaine depressed all cellular ... More
Receptor-mediated binding of leukocyte elastase by chondrocytes.
AuthorsBartholomew JS, Lowther DA
JournalArthritis Rheum
PubMed ID3034297
'Studies on the effect of leukocyte elastase on the metabolism of chondrocytes in culture have demonstrated that these cells possess a specific cell surface receptor for leukocyte-derived elastase. Purified elastase from rabbit and human leukocytes is capable of modulating the metabolism of the cell by causing a marked decrease in ... More
Enhancement of the activity of immunotoxins made with either ricin A chain or Pseudomonas exotoxin in human ovarian and epidermoid carcinoma cell lines.
AuthorsPirker R, FitzGerald DJ, Willingham MC, Pastan I
JournalCancer Res
PubMed ID3260128
'The present study evaluates whether the in vitro activity of immunotoxins can be enhanced by verapamil or by various antagonists of calmodulin (dansylcadaverine, trifluoperazine, chlorpromazine). The following immunotoxins made with either Pseudomonas exotoxin (PE), recombinant ricin A chain (rRTA), or ricin A chain (RTA) were used: HB21-PE and 454A12-rRTA that ... More
Oral mucosal pellicle. Adsorption and transpeptidation of salivary components to buccal epithelial cells.
AuthorsBradway SD, Bergey EJ, Jones PC, Levine MJ
JournalBiochem J
PubMed ID2572218
'The present investigation was carried out to examine the mechanism(s) whereby salivary molecules interact with human buccal epithelial cells. By utilizing antiserum against human parotid saliva, selected salivary components were detected by electrophoretic-transfer analysis of 1.5% SDS extracts of epithelial cells. Incubation of the cells and their aqueous cell-free extracts ... More
The growth and differentiation of cultured newborn rat keratinocytes.
AuthorsBaden HP, Kubilus J
JournalJ Invest Dermatol
PubMed ID6185597
'Keratinocytes were cultured from adult and newborn rat epidermis using the 3T3 feeder cell technique. By modifying culture conditions a long-lived line of newborn rat keratinocytes was developed which showed a plating efficiency of 40% and a doubling time of 16 h. The cells produced stratified colonies with tonofilaments, desmosomes, ... More
Intracellular infection by the human granulocytic ehrlichiosis agent inhibits human neutrophil apoptosis.
AuthorsYoshiie K, Kim HY, Mott J, Rikihisa Y
JournalInfect Immun
PubMed ID10678916
'In patients with human granulocytic ehrlichiosis (HGE), the HGE agent has been seen only in the peripheral blood granulocytes, which have a life span too short for ehrlichial proliferation. To determine if the HGE agent delays the apoptosis of human peripheral blood neutrophils for its advantage, peripheral blood granulocytes consisting ... More
Primary amines inhibit the triggering of B lymphocytes to antibody synthesis.
AuthorsJulian C, Speck NA, Pierce SK
JournalJ Immunol
PubMed ID6128364
'Transglutaminase enzyme activity was demonstrated in murine splenic B lymphocytes. Preincubation of cell cultures with primary amines that are known substrates for transglutaminase inhibited the triggering of murine B lymphocytes to form clones of antibody-producing plasma cells. B cells could be rescued from this inhibition by incubating cultures with anti-immunoglobulin ... More
Effects of transglutaminase substrates and inhibitors on the motility of demembranated reactivated spermatozoa.
Authorsde Lamirande E, Gagnon C
JournalGamete Res
PubMed ID2565284
'The effects of transglutaminase (TGase) substrates putrescine, dansylcadaverine, spermine, etc., and the TGase inhibitor cystamine were tested on the motility of demembranated mammalian spermatozoa. These products blocked within a few seconds the motility of demembranated reactivated spermatozoa at concentrations ranging from 0.25 to 5 mM. These minimal inhibitory concentrations could ... More
Analysis of the endocytic pathway upon intracellular transport of IgG molecules through Fc receptors.
AuthorsAthanassakis I, Protopapadakis E, Vassiliadis S
JournalJ Recept Signal Transduct Res
PubMed ID10071754
'The uniformly distributed Fc receptors (FcRs) on the surface of many cell types are involved in a variety of immune reactions by non-specifically facilitating the entry of antigen-specific IgG molecules to the cell. Such reactions may be beneficial to the organism when foreign antigens are involved, or harmful in cases ... More
The difference between platelet and plasma FXIII used to study the mechanism of platelet microvesicle formation.
AuthorsHolme PA, Brosstad F, Solum NO
JournalThromb Haemost
PubMed ID8115996
'The formation of microvesicles from platelets was induced either by activation of the complement system by a monoclonal antibody to CD9, or by incubation of platelets with the calcium ionophore A23187. A filter technique to isolate the microvesicles without plasma contamination is described. The microvesicles contained FXIIIa2 from the platelet ... More
Secretin internalization and adenosine 3',5'-monophosphate levels in pancreatic acinar cells.
AuthorsIzzo RS, Chen AI, Pellecchia C, Praissman M
JournalEndocrinology
PubMed ID2539974
'The internalization of [125I]secretin in pancreatic acinar cells was evaluated by differentiation of surface-bound and internalized radioligand with an acidified glycine buffer. The amount of surface-bound radioligand was 2-fold higher at 37 C than at 4 C between 15 and 60 min; internalized radioactivity was more than 10-fold greater at ... More
Cross-linking of cold-insoluble globulin by fibrin-stabilizing factor.
AuthorsMosher DF
JournalJ Biol Chem
PubMed ID1158872
'Cold-insoluble globulin (CI globulin) was purified from human plasma and identified on the basis of its sedimentation coefficient, electrophoretic mobility, and concentration in normal plasma. CI globulin was distinguished from antihemophilic factor (AHF) by amino acid analysis, position of elution from 4% agarose, and electrophoretic migration in polyacrylamide gels in ... More
16-kilodalton heparin binding (fibroblast) growth factor type one appears in a stable 40-kilodalton complex after receptor-dependent internalization.
AuthorsShi E, Kan M, Xu JM, McKeehan WL
JournalJ Biol Chem
PubMed ID2005115
'Incubation of 16-kDa 125I-labeled heparin binding (acidic fibroblast) growth factor type one (HBGF-1) with human hepatoma cells and normal rat hepatocytes resulted in the appearance of a stable 125I-labeled complex with an apparent molecular mass of 40 kDa. The complex could be isolated with specific antibodies against HBGF-1. In contrast ... More
Transglutaminase activity in aging articular chondrocytes and articular cartilage vesicles.
AuthorsRosenthal AK, Derfus BA, Henry LA
JournalArthritis Rheum
PubMed ID9153560
'OBJECTIVE: Transglutaminases (TGases) (E.C. 2.3.2.13) catalyze a posttranslational modification of proteins and are associated with biomineralization in growth plate cartilage. Type II TGase participates in the activation of latent transforming growth factor beta (TGFbeta), a crucial factor for both normal cartilage mineralization and the pathologic mineralization that results in calcium ... More
Tetanus toxin inhibits neuroexocytosis even when its Zn(2+)-dependent protease activity is removed.
AuthorsAshton AC, Li Y, Doussau F, Weller U, Dougan G, Poulain B, Dolly JO
JournalJ Biol Chem
PubMed ID8537412
'Tetanus toxin (TeTX) is a dichain protein that blocks neuroexocytosis, an action attributed previously to Zn(2+)-dependent proteolysis of synaptobrevin (Sbr) by its light chain (LC). Herein, its cleavage of Sbr in rat cerebrocortical synaptosomes was shown to be minimized by captopril, an inhibitor of certain metalloendoproteases, whereas this agent only ... More
A fluorescent substrate of transglutaminase for detection and characterization of glutamine acceptor compounds.
AuthorsPasternack R, Laurent HP, Rüth T, Kaiser A, Schön N, Fuchsbauer HL
JournalAnal Biochem
PubMed ID9193708
'A fluorescent dipeptide was designed to discover glutamine acceptor proteins of transglutaminase. Starting materials for synthesis were the commercially available compounds carbobenzoxy-L-glutaminylglycine (CBZ-Gln-Gly) and monodansylcadaverine (C-DNS) which were coupled to obtain CBZ-Gln-Gly-C-DNS 1 [1-N-(carbobenzoxy-L-glutaminylglycyl)-5-N- (5''-N'', N''-dimethylamino-1''-naphthalenesulfonyl)- diamidopentane]. The glutamine peptide is a substrate of bacterial transglutaminase from Streptoverticillium mobaraense as ... More
A fluorometric, high-performance liquid chromatographic assay for transglutaminase activity.
AuthorsFink ML, Shao YY, Kersh GJ
JournalAnal Biochem
PubMed ID1352948
'A fluorometric, high-performance liquid chromatographic assay for transglutaminase activity is described. The method uses the small synthetic peptide benzyloxycarbonyl-L-glutaminylglycine and the fluorescent amine monodansylcadaverine as substrates. Very small amounts of substrates and enzyme are required for this assay. The reaction product is separated from substrates on a reversed-phase, C-18 column, ... More
Post-translational modification of the hepatitis C virus core protein by tissue transglutaminase.
AuthorsLu W, Strohecker A, Ou Jh JH
JournalJ Biol Chem
PubMed ID11606596
'The hepatitis C virus (HCV) core protein is a structural protein that packages the viral genomic RNA. In this study, we demonstrate that a stable core protein dimer could be produced in liver cells. The production of this protein could be enhanced by calphostin C and serum deprivation. This protein ... More
Evidence for filaggrin as a component of the cell envelope of the newborn rat.
AuthorsRichards S, Scott IR, Harding CR, Liddell JE, Powell GM, Curtis CG
JournalBiochem J
PubMed ID3421939
'A substrate of transglutaminase, specific to the epidermis, was identified, by fluorescent and radioactive labelling with the lysine analogues dansylcadaverine and [14C]putrescine respectively, in newborn-rat epidermal homogenates and whole-skin organ cultures. The labelled analogues were preferentially incorporated into the stratum-corneum protein filaggrin in a Ca2+-dependent manner in both ''in vitro'' ... More
Enzymic preparation of protein G-peroxidase conjugates catalysed by transglutaminase.
AuthorsBechtold U, Otterbach JT, Pasternack R, Fuchsbauer HL
JournalJ Biochem (Tokyo)
PubMed ID10731690
'Transglutaminases (TGases, EC 2.3.2.13) have proved to be valuable enzymes for site-directed protein coupling via N(epsilon)-(gamma-L-glutamyl)lysine bonds. Their use in conjugate synthesis would overcome many problems caused by chemical reagents. In this approach, we show for the first time that two proteins with different functionalities, namely soybean peroxidase and protein ... More
Characteristics of the internalization and intracellular survival of Campylobacter jejuni in human epithelial cell cultures.
AuthorsKonkel ME, Hayes SF, Joens LA, Cieplak W
JournalMicrob Pathog
PubMed ID1297914
'The characteristics associated with the internalization and intracellular behavior of Campylobacter jejuni during short-term and long-term cultivation with INT 407 cells were examined. The internalization of C. jejuni by INT 407 cells was inhibited by cytochalasin dansylcadaverine, chemicals that disrupt microfilament formation and inhibit receptor cycling, respectively. Ammonium chloride and ... More
Fluorescence energy transfer detects changes in fibronectin structure upon surface binding.
AuthorsWolff C, Lai CS
JournalArch Biochem Biophys
PubMed ID2913946
'We report here the changes in intramolecular distances in human plasma fibronectin (Fn) detected, upon adsorption of the protein to the surface of the Cytodex dextran microcarrier, using a fluorescence energy transfer technique. The glutamine-3 residue, near the amino terminus of each chain, was labeled enzymatically with either monodansylcadaverine (dansyl) ... More
Brugia malayi and Acanthocheilonema viteae: antifilarial activity of transglutaminase inhibitors in vitro.
AuthorsRao UR, Mehta K, Subrahmanyam D, Vickery AC
JournalAntimicrob Agents Chemother
PubMed ID1687106
'The possible involvement of transglutaminase-catalyzed reactions in survival of adult worms, microfilariae (mf), and infective larvae of the filarial parasite Brugia malayi was studied in vitro by using the specific pseudosubstrate monodansylcadaverine (MDC) and the active-site inhibitors cystamine or iodoacetamide. These inhibitors significantly inhibited parasite mobility in a dose-dependent manner. ... More
Glutaminyl-peptide gamma-glutamyltransferase dependence of the uptake of trypsin-alpha-macroglobulin complexes by macrophages.
AuthorsOhlsson K, Polling A, Stenberg P
JournalHoppe Seylers Z Physiol Chem
PubMed ID6176517
'The canine alpha-macroglobulin 125I-trypsin complexes were prepared and exposed to canine alveolar macrophages. The binding of the complexes to cells was time- and dose-dependent. A rapid uptake and degradation of the bound complexes was evidenced by the finding of less than 20% cell-bound radioactivity after a 4 h incubation at ... More
Mechanism of autodegradation of cell-surface macromolecules shed by human melanoma cells.
AuthorsJohnston D, Bystryn JC
JournalExp Cell Res
PubMed ID6425072
'The mechanism of autodegradation of cell-surface macromolecules shed by human melanoma cells was studied by incubating radio-iodinated shed macromolecules with unlabeled sister cells and measuring the appearance of acid-soluble radioactivity. After a preliminary latent period of 1-3 h, degradation continually increased up to 24 h and was concentration-dependent. By contrast, ... More
Activation of transglutaminase during embryonic development.
AuthorsCariello L, Wilson J, Lorand L
JournalBiochemistry
PubMed ID6152182
'Incorporation of [3H]putrescine into proteins was shown to increase markedly in sea urchin eggs upon fertilization. Emetine, an inhibitor of protein synthesis, had no effect on the rate of protein labeling. However, the reaction could be prevented by the addition of 2-[3-(diallylamino)-propionyl]benzothiophene, a noncompetitive inhibitor of transglutaminase, and also by ... More
Probing the transglutaminase-mediated, posttranslational modification of proteins during development.
AuthorsCariello L, Velasco PT, Wilson J, Parameswaran KN, Karush F, Lorand L
JournalBiochemistry
PubMed ID1974146
'Sphaerechinus granularis eggs were fertilized in seawater in the presence of 0.2 mM dansylcadaverine, and development was allowed to take place with this compound in the medium. gamma-Glutamyldansylcadaverine, indicative of the utilization of the amine tracer by intrinsic transglutaminase, was isolated from the embryonic proteins, and identity of the product ... More
Adherence and internalization of Helicobacter pylori by HEp-2 cells.
AuthorsEvans DG, Evans DJ, Graham DY
JournalGastroenterology
PubMed ID1568565
'Helicobacter pylori colonizes the mucous layer of the stomach and the surface of gastric mucous cells. Although H. pylori is not generally thought of as invasive, it has been observed in the lamina propria and within vacuoles in the cytoplasm of epithelial cells. The authors report that isolates of H. ... More
Hamycin inhibits IL-8-induced biologic response by modulating its receptor in human polymorphonuclear neutrophils.
AuthorsManna SK, Samanta S, Samanta AK
JournalJ Immunol
PubMed ID9366432
'IL-8, a neutrophil chemotactic agent, is involved in a large number of neutrophil-driven acute and chronic inflammatory diseases. We have found that hamycin, an antifungal agent, reduces IL-8-induced migration and binding of 125I-labeled IL-8 to neutrophils by 66 and 75%, respectively. Other IL-8-induced biologic functions, such as superoxide generation, intracellular ... More
Transglutaminase activity in human and rabbit ear comedogenesis: a histochemical study.
AuthorsDe Young L, Ballaron S, Epstein W
JournalJ Invest Dermatol
PubMed ID6142072
'Using monodansylcadaverine as a fluorescent, lysine-substrate analogue, alterations in the histochemical profile of transglutaminase activity during human and coal tar-induced rabbit ear comedogenesis were examined. When compared to the normal pilosebaceous follicles of both species, a marked increase in enzyme-specific fluorescence in the hyperplastic epidermal lining and in the epithelium ... More
Purification and characterization of six cytochrome P-450 isozymes from human liver microsomes.
'Six cytochrome P-450 (P-450) isozymes were purified to electrophoretic homogeneity from the livers of four human organ donors, with three of these isozymes purified from a single individual. Differences were noted between all six P-450s for some or all of the parameters determined by the techniques of sodium dodecyl sulfate-polyacrylamide ... More
Effects of transglutaminase or phospholipase A2 inhibitors on down-regulation of prolactin receptors and stimulation of casein and DNA synthesis in mammary gland explants.
AuthorsDusanter-Fourt I, Djiane J, Houdebine LM
JournalMol Cell Endocrinol
PubMed ID6138286
'A number of compounds have been found to inhibit the internalization of alpha 2-macroglobulin and/or epidermal growth factor into fibroblasts. Using the same inhibitor we tried to block the down-regulation of prolactin receptors, supposed to mirror internalization of PRL receptors, in order to investigate the possible role of internalization in ... More
ATP-dependent acidification of membrane vesicles isolated from purified rat liver lysosomes. Acidification activity requires phosphate.
AuthorsSchneider DL
JournalJ Biol Chem
PubMed ID6218167
'Membrane vesicles were isolated from purified liver lysosomes of rats treated with Triton WR-1339. In order to preserve ATP-dependent acidification activity, proteolysis of membranes was minimized by adding protease inhibitors and by centrifuging to form dilute bands of vesicles rather than highly concentrated pellets. The membrane vesicle fraction represented about ... More
Keratin cross-linking and epidermal transglutaminase. A review with observations on the histochemical and immunochemical localization of the enzyme.
AuthorsBuxman MM, Wuepper KD
JournalJ Invest Dermatol
PubMed ID239069
'Enzymes known as transglutaminases mediate cross-linking of polypeptide chains by epsilon-(gamma-glutamyl) lysine bonds. Such bonds stabilize structural proteins of many tissues; transglutaminases specific for these tissues have been identified. A calcium- and sulfhydryl-dependent transglutaminase with a molecular weight of 55,000 has been purified from bovine snout epidermis and used to ... More
Identification of a novel transglutaminase from the filarial parasite Brugia malayi and its role in growth and development.
AuthorsMehta K, Rao UR, Vickery AC, Fesus L
JournalMol Biochem Parasitol
PubMed ID1354328
'Recently, we reported the presence of a putative transglutaminase in adult female worms of Brugia malayi [1]. The enzyme activity was shown to be essential for in utero growth and development of microfilariae. Here, we demonstrate that adult worms of B. malayi have a large amount of epsilon-(gamma-glutamyl)lysine isopeptide bonds, ... More
Differentiation of acid-pH-dependent and -nondependent entry pathways for mouse hepatitis virus.
AuthorsKooi C, Cervin M, Anderson R
JournalVirology
PubMed ID1845820
'Early events of infection of MHV were studied in comparison with those of VSV, which is known to enter cells by an endocytic pathway. Treatment of mouse L-2 fibroblasts with ammonium chloride, chloroquine, or dansylcadaverine inhibited infection of MHV to a much lesser degree than that of VSV, suggesting a ... More
Mechanisms of ligand-induced desensitization of the 5-hydroxytryptamine(2A) receptor.
AuthorsHanley NR, Hensler JG
JournalJ Pharmacol Exp Ther
PubMed ID11805206
'We have examined the cellular processes underlying the desensitization of the 5-hydroxytryptamine (5-HT)(2A) receptor induced by agonist or antagonist exposure. Treatment of C6 glioma cells with either 5-HT or the 5-HT(2A) receptor antagonist ketanserin resulted in an attenuation in 5-HT(2A) receptor function, specifically the accumulation of inositol phosphates stimulated by ... More
Involvement of hormone processing in insulin-activated glucose transport by isolated cardiac myocytes.
AuthorsEckel J, Reinauer H
JournalBiochem J
PubMed ID3277615
'Isolated muscle cells from adult rat heart were used to study the relationship between myocardial insulin processing and insulin action on 3-O-methylglucose transport at 37 degrees C. Internalization of the hormone as measured by determination of the non-dissociable fraction of cell-bound insulin increased linearly up to 10 min, reaching a ... More
Metabolism of high-density lipoproteins in cultured rat luteal cells.
AuthorsRajan VP, Menon KM
JournalBiochim Biophys Acta
PubMed ID3620487
'The uptake of cholesterol from high-density lipoproteins (HDL) labeled with 125I and [3H]cholesterol was examined in cultured rat luteal cells. Luteal cells were incubated with labeled HDL, following which the metabolic fate of the apolipoproteins and cholesterol moieties of the receptor-bound HDL were examined. About 50% of the originally bound ... More
Evidence for participation of transglutaminase in receptor-mediated endocytosis.
AuthorsLevitzki A, Willingham M, Pastan I
JournalProc Natl Acad Sci U S A
PubMed ID6156455
'We report evidence that the enzyme transglutaminase (glutaminyl-peptide gamma-glutamyltransferase; R-glutaminyl-peptide:amine gamma-glutamyltransferase, EC2.3.2.13) participates in receptor-mediated endocytosis. Clustering and internalization of rhodamine-labeled alpha 2-macroglobulin (R alpha 2 M) in normal rat kidney (NRK) cells is inhibited by a wide spectrum of compounds that inhibit transglutaminases, including that from NRK cells. The ... More
Isolation, purification and characterization of bovine epidermal transglutaminase.
AuthorsBuxman MM, Wuepper KD
JournalBiochim Biophys Acta
PubMed ID12807
'A crosslinking enzyme, epidermal transglutaminase, was isolated from soluble proteins of glabrous cow snout epidermis. This enzyme stabilized fibrin clots rendering them insoluble in 2% acetic acid. It also catalyzed the incorporation of the fluorescent amine, dansyl cadaverine, into casein. Epidermal transglutaminase was purified by chromatography upon DEAE-Sephadex A-50, zone ... More
Enhanced susceptibility to transglutaminase reaction of alpha-lactalbumin in the molten globule state.
AuthorsMatsumura Y, Chanyongvorakul Y, Kumazawa Y, Ohtsuka T, Mori T
JournalBiochim Biophys Acta
PubMed ID8547351
'The susceptibility of alpha-lactalbumin to transglutaminase reactions was studied using an enzyme from Streptoverticillium which can catalyze the reactions irrespective of the presence or absence of Ca2+. Transglutaminase-catalyzed polymerization of alpha-lactalbumin in the native state occurred to a very limited extent. Transformation from the native state to the molten globule ... More
Control mechanisms governing the infectivity of Chlamydia trachomatis for HeLa cells: mechanisms of endocytosis.
AuthorsWard ME, Murray A
JournalJ Gen Microbiol
PubMed ID6470672
'The mechanism by which Chlamydia trachomatis is endocytosed by host cells is unclear. Studies of the kinetics of chlamydial attachment and uptake in the susceptible HeLa 229 cell line showed that chlamydial endocytosis was rapid and saturable but limited by the slow rate of chlamydial attachment. To overcome this limitation ... More
The major structural proteins of cod (Gadus morhua) eggshells and protein crosslinking during teleost egg hardening.
AuthorsOppen-Berntsen DO, Helvik JV, Walther BT
JournalDev Biol
PubMed ID2303164
'The highly hydrophobic protein aggregate which constitutes the fish eggshell has for the first time been quantitatively solubilized. This study shows that the nonactivated eggshell from cod is composed primarily of only three protein monomers, designated alpha (74 kDa) beta (54 kDa) and gamma (47 kDa). Protein extraction studies of ... More
Catalytic properties of a calmodulin-regulated transglutaminase from human platelet and chicken gizzard.
AuthorsPuszkin EG, Raghuraman V
JournalJ Biol Chem
PubMed ID2866189
'The kinetic parameters and some enzymatic characteristics of human platelet and chicken gizzard transglutaminases were determined. Activity of the transglutaminases was regulated by calmodulin. These enzymes co-isolated with alpha-actinin and were dissociated from alpha-actinin by gel filtration and absorption onto a calmodulin affinity column. Silver-stained polyacrylamide gels showed that the ... More
Purification and partial characterization of 76 kDa transglutaminase in the egg envelope (chorion) of rainbow trout, Oncorhynchus mykiss.
AuthorsHa CR, Iuchi I
JournalJ Biochem (Tokyo)
PubMed ID9443809
'Transglutaminase (TGase), responsible for crosslinking between proteins, is known to be localized exclusively in the egg envelope (chorion) of rainbow trout, Oncorhynchus mykiss, and probably participates in the post-fertilization chorion hardening. We purified the TGase from unfertilized egg chorions by sequential chromatography using SP-Sepharose, Q-Sepharose, and TSK-gel G3000SWXL columns. The ... More
Role of endocytosis in the activation of the extracellular signal-regulated kinase cascade by sequestering and nonsequestering G protein-coupled receptors.
AuthorsPierce KL, Maudsley S, Daaka Y, Luttrell LM, Lefkowitz RJ
JournalProc Natl Acad Sci U S A
PubMed ID10677489
'Acting through a number of distinct pathways, many G protein-coupled receptors (GPCRs) activate the extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase (MAPK) cascade. Recently, it has been shown that in some cases, clathrin-mediated endocytosis is required for GPCR activation of the ERK/MAPK cascade, whereas in others it is not. Accordingly, we ... More
Requirement for transglutaminase in progesterone-induced decidualization of human endometrial stromal cells.
AuthorsFujimoto M, Kanzaki H, Nakayama H, Higuchi T, Hatayama H, Iwai M, Kaneko Y, Mori T, Fujita J
JournalEndocrinology
PubMed ID8603579
'Differentiation of endometrial stromal cells (decidualization) is essential for embryo implantation and maintenance of pregnancy. By sequential complementary DNA subtractive hybridization, one of the messenger RNAs (mRNA) induced by progesterone in human endometrial stromal cells decidualized in vitro was identified as that of a tissue transglutaminase type II (TGase). TGase ... More
Different intracellular and intranuclear transport of triiodothyronine enantiomers in rat skeletal myoblasts.
AuthorsPontecorvi A, Lakshmanan M, Robbins J
JournalEndocrinology
PubMed ID3197649
'The mechanism(s) responsible for the different biological potency of L- and D-T3 was investigated in rat L6E9 myoblasts. After incubation with intact cells at 37 C L-T3 cellular and nuclear uptakes were 91% and 70% higher than those of D-T3, respectively, but values for nuclear uptake as a fraction of ... More
Interaction of insulin-like growth factor I/somatomedin-C with cultured rat chondrocytes: receptor binding and internalization.
AuthorsSchalch DS, Sessions CM, Farley AC, Masakawa A, Emler CA, Dills DG
JournalEndocrinology
PubMed ID3004923
'We have characterized the interaction of insulin-like growth factor I/somatomedin C (IGF-I/Sm-C) with its plasma membrane receptors on cultured rat chondrocytes. Our studies have demonstrated that [125I]IGF-I/Sm-C binding to these receptors is a relatively specific, reversible, and time-, temperature-, pH-, and concentration-dependent process. Insulin displaces [125I]IGF-I/Sm-C from its receptors on ... More
Functional coupling between a bafilomycin A1-sensitive proton pump and a probenecid-sensitive folate transporter in human placental choriocarcinoma cells.
AuthorsPrasad PD, Mahesh VB, Leibach FH, Ganapathy V
JournalBiochim Biophys Acta
PubMed ID8031869
'The mechanism of transport of 5-methyltetrahydrofolate, the predominant form of folate in human blood, was investigated in JAR human placental choriocarcinoma cells. Northern analysis revealed the presence of the folate receptor mRNA in these cells. Accumulation of 5-methyltetrahydrofolate in these cells was saturable and inhibited by other folate analogs. The ... More
Ehrlichia chaffeensis and E. sennetsu, but not the human granulocytic ehrlichiosis agent, colocalize with transferrin receptor and up-regulate transferrin receptor mRNA by activating iron-responsive protein 1.
AuthorsBarnewall RE, Ohashi N, Rikihisa Y
JournalInfect Immun
PubMed ID10225882
'Ehrlichia chaffeensis and E. sennetsu are genetically divergent obligatory intracellular bacteria of human monocytes and macrophages, and the human granulocytic ehrlichiosis (HGE) agent is an obligatory intracellular bacterium of granulocytes. Infection with both E. chaffeensis and E. sennetsu, but not HGE agent, in the acute monocytic leukemia cell line THP-1 ... More
Role of cell membrane composition in receptor-mediated internalization of vesicular stomatitis virus in human HEp-2 cells.
AuthorsPottathil R, Gutierrez PL, Davis LH, Chandrabose KA
JournalJ Biol Chem
PubMed ID2985585
'The role of essential fatty acids in membrane functions related to receptor-mediated endocytosis of vesicular stomatitis virus (VSV) was investigated using a human laryngeal carcinoma cell line (HEp-2) grown in chemically defined serum-free medium (DM) to deplete their essential fatty acid contents. VSV replicated much less effectively in HEp-2 cells ... More
Effect of transglutaminase substrates and polyamines on the cellular sequestration and processing of follicle-stimulating hormone by rat Sertoli cells.
AuthorsDias JA
JournalBiol Reprod
PubMed ID2874845
'Transglutaminase (TGase) substrates monodansyl cadaverine (MDC, monodansyl-1,5 diaminopentane) and methylamine (MA) and polyamines (PA) were tested for their effects on the cellular processing of radioiodinated human follicle-stimulating hormone (125I-hFSH). Specifically bound 125I-hFSH that could be released from cells during 10-min incubation period with acidified (pH 3.9) Hanks balanced-salt solution was ... More
Keratolinin: the soluble substrate of epidermal transglutaminase from human and bovine tissue.
AuthorsZettergren JG, Peterson LL, Wuepper KD
JournalProc Natl Acad Sci U S A
PubMed ID6141559
'Substrates of human and bovine epidermal transglutaminase (glutaminyl-peptide gamma-glutamyltransferase, R-glutaminyl-peptide:amine-gamma-glutamyltransferase, EC 2.3.2.13) were isolated and purified by ion exchange chromatography and preparative zone electrophoresis. These substrates of Mr 36,000, which we propose to call keratolinin, incorporated dansylcadaverine and were precipitated by antibody. Keratolinin is ultimately polymerized on the inner leaflet ... More
Peritoneal accumulation of infused stroma-free hemoglobin. Potential toxicity of an oxygen-carrying substitute.
AuthorsVelky TS, Lee ES, Maffuid PW, Robinson GT, Yang JC, Greenburg AG
JournalArch Surg
PubMed ID3827578
'The efficacy of stroma-free hemoglobin (SFH) as an oxygen-carrying red blood cell substitute in shock and trauma remains inconclusive. A major problem is the retention of sufficient intravascular persistence. The sites and mechanisms for clearance of SFH or its chemically modified variants are not well characterized. Capillary leakage has been ... More
Inhibition of blood coagulation factor XIIIa-mediated cross-linking between fibronectin and collagen by polyamines.
AuthorsMosher DF, Schad PE, Kleinman HK
JournalJ Supramol Struct
PubMed ID548650
'Soluble fibronectin is found in body fluids and media of cultured adherent cells. Insoluble fibronectin is found in tissue stroma and in extracellular matrices of cultured cells. Fibronectin is a substrate for factor XIIIa (plasma transglutaminase) and can be cross-linked to collagen and to the alpha chain of fibrin. We ... More
Transglutaminase activity in equine strongyles and its potential role in growth and development.
'Transglutaminases (E.C. 2.3.3.13) are a family of Ca(2+)-dependent enzymes that stabilize protein structure by catalyzing the formation of isopeptide bonds. A novel form of transglutaminase has been identified and characterized that seem to play an important role in growth, development, and molting in adult and larval stages of filarial nematodes. ... More
Tissue transglutaminase-dependent posttranslational modification of the retinoblastoma gene product in promonocytic cells undergoing apoptosis.
AuthorsOliverio S, Amendola A, Di Sano F, Farrace MG, Fesus L, Nemes Z, Piredda L, Spinedi A, Piacentini M
JournalMol Cell Biol
PubMed ID9315663
'The retinoblastoma gene product (pRB) plays an important role in controlling both cell release from the G1 phase and apoptosis. We show here that in the early phases of apoptosis, pRB is posttranslationally modified by a tissue transglutaminase (tTG)-catalyzed reaction. In fact, by employing a novel haptenized lysis synthetic substrate ... More
Cellular events concurrent with Porphyromonas gingivalis invasion of oral epithelium in vitro.
AuthorsSandros J, Madianos PN, Papapanou PN
JournalEur J Oral Sci
PubMed ID8930584
'The aim of the present study was to elucidate events related to receptor function, signal transmission and cytoskeletal rearrangements concurrent with Porphyromonas gingivalis invasion of oral epithelial cells in vitro. Porphyromonas gingivalis strain FDC 381 and the KB cell line (ATCC CCL 17) were used in a previously described antibiotic ... More
Transglutaminase catalyses the modification of glutamine side chains in the C-terminal region of bovine beta-lactoglobulin.
AuthorsCoussons PJ, Price NC, Kelly SM, Smith B, Sawyer L
JournalBiochem J
PubMed ID1350436
'The transglutaminase-catalysed incorporation of primary amines (putrescine and monodansylcadaverine) into bovine beta-lactoglobulin has been studied. In the presence of 1 mM-dithiothreitol between 1 and 2 mol of amine can be incorporated per mol of beta-lactoglobulin subunit. There is very little incorporation of amines in the absence of reducing agent. By ... More
Transglutaminase activity in pancreatic islets.
AuthorsGomis R, Sener A, Malaisse-Lagae F, Malaisse WJ
JournalBiochim Biophys Acta
PubMed ID6138101
'1. Pancreatic islet homogenates catalyze, in a Ca2+-dependent fashion, the incorporation of [2,5-3H]histamine, [1,4-14C]putrescine, [1,2-3H]agmatine, [14C]methylamine and L-[U-14C]lysine in N,N-dimethylcasein. 2. Using [2,5-3H]histamine as the amine donor, the Km for Ca2+ and histamine amounts to 90 microM and 0.7 mM, respectively. 3. The incorporation of [2,5-3H]histamine into N,N-dimethylcasein is inhibited ... More
Transglutaminase-catalyzed matrix cross-linking in differentiating cartilage: identification of osteonectin as a major glutaminyl substrate.
AuthorsAeschlimann D, Kaupp O, Paulsson M
JournalJ Cell Biol
PubMed ID7730416
'The expression of tissue transglutaminase in skeletal tissues is strictly regulated and correlates with chondrocyte differentiation and cartilage calcification in endochondral bone formation and in maturation of tracheal cartilage (Aeschlimann, D., A. Wetterwald, H. Fleisch, and M. Paulsson. 1993. J. Cell Biol. 120:1461-1470). We now demonstrate the transglutaminase reaction product, ... More
An immunohistochemical and histochemical study of cytokeratin, involucrin and transglutaminase in seborrhoeic keratosis.
AuthorsBroekaert D, Leigh IM, Lane EB, Van Muijen GN, Ramaekers FC, De Bersaques J, Coucke P
JournalArch Dermatol Res
PubMed ID7506018
'The mode of differentiation of seborrhoeic keratoses was investigated by immunohistochemical staining using cytokeratin (CK) polypeptide-specific monoclonal antibodies and an antibody specific for the particulate form of epidermal transglutaminase (ETgase), and by applying an anti-human involucrin serum. The role played by (E)Tgase was further evaluated using an activity assay based ... More
[3H]Fucose incorporation by healing skin wounds and the effect of transglutaminase inhibitors.
AuthorsBowness JM
JournalCan J Biochem
PubMed ID6127151
'Punch wounds (3 mm) were made in the skin of rats and the animals were killed after 1 or 3 days. Plugs (4 mm) of wounded and unwounded skin were incubated in vitro with [3H]fucose. The labelled plugs were homogenized and subjected to sequential extraction with buffered salt solutions, ethanol-ether, ... More
beta-Crystallin: endogenous substrate of lens transglutaminase. Characterization of the acyl-donor site in the beta Bp chain.
AuthorsBerbers GA, Bentlage HC, Brans AM, Bloemendal H, de Jong WW
JournalEur J Biochem
PubMed ID6136410
'Incubation of calf lens cortex homogenate with [14C]putrescine or dansylcadaverine, followed by two-dimensional gel electrophoresis and fluorography, enabled the identification of three different beta-crystallin chains as the endogenous substrates of Ca2+-dependent lens transglutaminase (R-glutaminyl-peptide:amine-gamma-glutamylyltransferase, EC 2.3.2.13). One of these is beta Bp, the predominant subunit of beta-crystallin, of which the ... More
Lysosomal and non-lysosomal pathways of intracellular insulin degradation in isolated rat hepatocytes.
AuthorsDraznin B, Todd WW, Leitner JW, Toothaker DR
JournalHorm Res
PubMed ID6765590
'The amount of 125I-insulin associated with freshly isolated hepatocytes was increased 50% in the presence of 0.2 mM chloroquine (CQ) after 2 h of incubation. The degradation of insulin by the hepatocytes incubated with CQ was significantly diminished as compared with control cells. Hepatocytes incubated with 125I-insulin in the presence ... More
Rapid increase of cellular UDP-glucuronide after mitogen stimulation of quiescent 3T3 mouse fibroblasts.
AuthorsZaharevitz DW, Chisena CA, Cysyk RL
JournalBiochem Int
PubMed ID2196057
'Chromatographic analysis of the acid soluble nucleotide pool in 3T3 fibroblasts 30 minutes after serum stimulation revealed one component that was increased compared to quiescent controls. This component was identified as UDP-glucuronide by chromatographic and chemical means. The time course and magnitude of the serum stimulated increase in UDP-glucuronide is ... More
Interaction between rat alpha 1 fetoprotein and fluorescent derivatives of estrone in relation to the position and type of the fluorescent label.
AuthorsDesfosses B, Herve F, Moenner M, Urios P, Cittanova N, Dessen P
JournalJ Steroid Biochem
PubMed ID6200695
'A series of derivatives of estrone with fluorescent dyes (dansyl or coumarin) coupled at different positions on the steroid molecule, have been synthesized. These derivatives were tested for their quantum yields and their binding properties were determined with respect to rat alpha 1 fetoprotein. Derivatives at C-3 (estrone-3-hemisuccinate-dansyl-cadaverine and estrone-3-dansyl) ... More
Transglutaminase-catalyzed reaction is important for molting of Onchocerca volvulus third-stage larvae.
AuthorsLustigman S, Brotman B, Huima T, Castelhano AL, Singh RN, Mehta K, Prince AM
JournalAntimicrob Agents Chemother
PubMed ID8540691
'Highly insoluble proteins, which are probably cross-linked, are common in the cuticle and epicuticle of filarial parasites and other nematode species. We have investigated the possible involvement of transglutaminase (TGase)-catalyzed reactions in the development of Onchocerca volvulus fourth-stage larvae (L4) by testing the effects of TGase inhibitors on the survival ... More
The effect of inhibitors of insulin processing on generation of insulin intermediate products from human fibroblast as detected by high performance liquid chromatography (HPLC).
AuthorsKitabchi AE, Stentz FB
JournalBiochem Biophys Res Commun
PubMed ID3885945
'To assess the role of various modulators of insulin processing on cell-associated A14-125I-insulin intermediates in human fibroblasts, we have studied the effect of N-ethylmaleimide (NEM), chloroquine, bacitracin, dansylcadavarine, and phenylarsine oxide on generation of these intermediate products with the use of HPLC. NEM completely inhibited generation of intermediate peaks or ... More
Transglutaminase-catalyzed incorporation of host proteins in Brugia malayi microfilariae.
AuthorsMehta K, Chandrashekar R, Rao UR
JournalMol Biochem Parasitol
PubMed ID8919999
'Recently, we have characterized and purified a novel transglutaminase (pTGase) from adults of the filarial worms Brugia malayi. pTGase-catalyzed reactions seem to play an essential role during in utero growth and development of microfilariae. The results presented here demonstrate that exudates from the peritoneal cavity of jirds, the site where ... More
Labeling of epsilon-lysine crosslinking sites in proteins with peptide substrates of factor XIIIa and transglutaminase.
AuthorsParameswaran KN, Velasco PT, Wilson J, Lorand L
JournalProc Natl Acad Sci U S A
PubMed ID1978326
'Peptides patterned on the N-terminal sequence of fibronectin were synthesized and tested for amine acceptor qualities in reactions with dansylcadaverine catalyzed either by coagulation factor XIIIa or intracellular transglutaminase (protein-glutamine:amine gamma-glutamyltransferase, EC 2.3.2.13). On the basis of inverse half-saturations of the enzymes, the order of acceptor substrate affinity for factor ... More
Isolation of a sodium dodecyl sulfate-insoluble transglutaminase substrate from liver plasma membranes.
AuthorsTyrrell DJ, Sale WS, Slife CW
JournalJ Biol Chem
PubMed ID2892834
'Rat liver plasma membranes contain transglutaminase activity and a large molecular weight protein complex which serves as a substrate for this enzyme. When plasma membranes were solubilized in sodium dodecyl sulfate and disulfide-reducing agents the transglutaminase substrate was recovered in the detergent-insoluble fraction. The insolubility of the complex suggested that ... More