Higher order Rab programming in phagolysosome biogenesis.
AuthorsRoberts EA, Chua J, Kyei GB, Deretic V
JournalJ Cell Biol
PubMed ID16982798
'Phagosomes offer kinetically and morphologically tractable organelles to dissect the control of phagolysosome biogenesis by Rab GTPases. Model phagosomes harboring latex beads undergo a coordinated Rab5-Rab7 exchange, which is akin to the process of endosomal Rab conversion, the control mechanisms of which are unknown. In the process of blocking phagosomal ... More
Human IRGM induces autophagy to eliminate intracellular mycobacteria.
AuthorsSingh SB, Davis AS, Taylor GA, Deretic V
JournalScience
PubMed ID16888103
Immunity-related p47 guanosine triphosphatases (IRG) play a role in defense against intracellular pathogens. We found that the murine Irgm1 (LRG-47) guanosine triphosphatase induced autophagy and generated large autolysosomal organelles as a mechanism for the elimination of intracellular Mycobacterium tuberculosis. We also identified a function for a human IRG protein in ... More
Assays to assess autophagy induction and fusion of autophagic vacuoles with a degradative compartment, using monodansylcadaverine (MDC) and DQ-BSA.
AuthorsVázquez CL, Colombo MI,
JournalMethods Enzymol
PubMed ID19200877
In this chapter we describe the use of monodasylcadaverine (MDC) and DQ-BSA, two practical and convenient tools to study the autophagic pathway. MDC is a lysosomotropic compound useful for the identification of autophagic vesicles by fluorescence microscopy and, in addition, to assess autophagy induction via the accumulation of MDC-labeled vacuoles. ... More
Immune complex relay by subcapsular sinus macrophages and noncognate B cells drives antibody affinity maturation.
AuthorsPhan TG, Green JA, Gray EE, Xu Y, Cyster JG,
JournalNat Immunol
PubMed ID19503106
Subcapsular sinus (SCS) macrophages capture antigens from lymph and present them intact for B cell encounter and follicular delivery. However, the properties of SCS macrophages are poorly defined. Here we show SCS macrophage development depended on lymphotoxin-alpha1beta2, and the cells had low lysosomal enzyme expression and retained opsonized antigens on ... More
In vivo visualization of albumin degradation in the proximal tubule.
AuthorsSlattery C, Lee A, Zhang Y, Kelly DJ, Thorn P, Nikolic-Paterson DJ, Tesch GH, Poronnik P,
JournalKidney Int
PubMed ID18800029
Albuminuria is a key marker of renal injury and a major risk factor for cardiovascular disease. In vivo imaging techniques with fluorescent albumin have allowed visualization of its movement within the whole kidney but they could not distinguish between intact and degraded albumin. To visualize albumin degradation in proximal tubular ... More
A contiguous compartment functions as endoplasmic reticulum and endosome/lysosome in Giardia lamblia.
AuthorsAbodeely M, DuBois KN, Hehl A, Stefanic S, Sajid M, DeSouza W, Attias M, Engel JC, Hsieh I, Fetter RD, McKerrow JH,
JournalEukaryot Cell
PubMed ID19749174
The dynamic evolution of organelle compartmentalization in eukaryotes and how strictly compartmentalization is maintained are matters of ongoing debate. While the endoplasmic reticulum (ER) is classically envisioned as the site of protein cotranslational translocation, it has recently been proposed to have pluripotent functions. Using transfected reporter constructs, organelle-specific markers, and ... More
A multienzyme network functions in intestinal protein digestion by a platyhelminth parasite.
AuthorsDelcroix M, Sajid M, Caffrey CR, Lim KC, Dvorák J, Hsieh I, Bahgat M, Dissous C, McKerrow JH
JournalJ Biol Chem
PubMed ID17028179
Proteases frequently function not only as individual enzymes but also in cascades or networks. A notable evolutionary switch occurred in one such protease network that is involved in protein digestion in the intestine. In vertebrates, this is largely the work of trypsin family serine proteases, whereas in invertebrates, cysteine proteases ... More
SORLA regulates endosomal trafficking and oncogenic fitness of HER2.
Authors
JournalNat Commun
PubMed ID31138794
Lysosomal Signaling Licenses Embryonic Stem Cell Differentiation via Inactivation of Tfe3.
Authors
JournalCell Stem Cell
PubMed ID30595499
INPP4B promotes PI3Kα-dependent late endosome formation and Wnt/β-catenin signaling in breast cancer.
Authors
JournalNat Commun
PubMed ID34035258
Folliculin impairs breast tumor growth by repressing TFE3-dependent induction of the Warburg effect and angiogenesis.
Authors
JournalJ Clin Invest
PubMed ID34779410
A mitochondria-targeted caffeic acid derivative reverts cellular and mitochondrial defects in human skin fibroblasts from male sporadic Parkinson's disease patients.
Authors
JournalRedox Biol
PubMed ID34147843
Intracellular localisation of Mycobacterium tuberculosis affects efficacy of the antibiotic pyrazinamide.
Authors
JournalNat Commun
PubMed ID34155215
Elaiophylin, a novel autophagy inhibitor, exerts antitumor activity as a single agent in ovarian cancer cells.
Authors
JournalAutophagy
PubMed ID25893854
Cysteamine-bicalutamide combination therapy corrects proximal tubule phenotype in cystinosis.
Authors
JournalEMBO Mol Med
PubMed ID34165243
MCOLN1/TRPML1 finely controls oncogenic autophagy in cancer by mediating zinc influx.
Authors
JournalAutophagy
PubMed ID33890549
Diclofenac impairs autophagic flux via oxidative stress and lysosomal dysfunction: Implications for hepatotoxicity.