CD34+ corneal stromal cells are bone marrow-derived and express hemopoietic stem cell markers.
AuthorsSosnová M, Bradl M, Forrester JV
JournalStem Cells
PubMed ID15790772
'Previous studies have suggested that corneal stromal keratocytes express the CD34 antigen. We wished to investigate CD34 antigen expression in normal mouse cornea using dual- and triple-staining techniques. Whole-mount preparations of mouse and rat corneas were examined with confocal microscopy using single, dual, or triple immunostaining to study their morphology, ... More
Hierarchy of protein assembly at the vertex ring domain for yeast vacuole docking and fusion.
AuthorsWang L, Merz AJ, Collins KM, Wickner W
JournalJ Cell Biol
PubMed ID12566429
'Vacuole tethering, docking, and fusion proteins assemble into a "vertex ring" around the apposed membranes of tethered vacuoles before catalyzing fusion. Inhibitors of the fusion reaction selectively interrupt protein assembly into the vertex ring, establishing a causal assembly hierarchy: (a) The Rab GTPase Ypt7p mediates vacuole tethering and forms the ... More
Remodeling of organelle-bound actin is required for yeast vacuole fusion.
AuthorsEitzen G, Wang L, Thorngren N, Wickner W
JournalJ Cell Biol
PubMed ID12177043
Actin participates in several intracellular trafficking pathways. We now find that actin, bound to the surface of purified yeast vacuoles in the absence of cytosol or cytoskeleton, regulates the last compartment mixing stage of homotypic vacuole fusion. The Cdc42p GTPase is known to be required for vacuole fusion. We now ... More
Plasma membrane surface increases with tonic stretch of alveolar epithelial cells.
AuthorsFisher JL, Levitan I, Margulies SS
JournalAm J Respir Cell Mol Biol
PubMed ID15016618
Cyclic stretch stimulates numerous responses in alveolar epithelial cells--some beneficial, some injurious--often through mechanosensitive membrane-associated proteins such as stretch-activated ion channels. Tonic stretch, in contrast, stimulates only some of these responses. In this study, we hypothesized that the plasma membranes of alveolar epithelial cells expand during tonic stretch, not only ... More
Imaging remodeling of the actin cytoskeleton in vascular smooth muscle cells after mechanosensitive arteriolar constriction.
AuthorsFlavahan NA, Bailey SR, Flavahan WA, Mitra S, Flavahan S
JournalAm J Physiol Heart Circ Physiol
PubMed ID15388507
Experiments were performed to determine whether remodeling of the actin cytoskeleton contributes to arteriolar constriction. Mouse tail arterioles were mounted on cannulae in a myograph and superfused with buffer solution. The alpha1-adrenergic agonist phenylephrine (0.1-1 micromol/l) caused constriction that was unaffected by cytochalasin D (300 nmol/l) or latrunculin A (100 ... More
Dissecting cellular processes using small molecules: identification of colchicine-like, taxol-like and other small molecules that perturb mitosis.
BACKGROUND: Understanding the molecular mechanisms of complex cellular processes requires unbiased means to identify and to alter conditionally gene products that function in a pathway of interest. Although random mutagenesis and screening (forward genetics) provide a useful means to this end, the complexity of the genome, long generation time and ... More
α1AMP-Activated Protein Kinase Protects against Lipopolysaccharide-Induced Endothelial Barrier Disruption via Junctional Reinforcement and Activation of the p38 MAPK/HSP27 Pathway.
Authors
JournalInt J Mol Sci
PubMed ID32759774
Spatial confinement downsizes the inflammatory response of macrophages.
Authors
JournalNat Mater
PubMed ID30349032
Serotonin receptor 4 regulates hippocampal astrocyte morphology and function.
Authors
JournalGlia
PubMed ID33156956
Low Cell-Matrix Adhesion Reveals Two Subtypes of Human Pluripotent Stem Cells.
Authors
JournalStem Cell Reports
PubMed ID30008324
Mechanostimulation Promotes Nuclear and Epigenetic Changes in Oligodendrocytes.
Authors
JournalJ Neurosci
PubMed ID26791211
Ketamine Corrects Stress-Induced Cognitive Dysfunction through JAK2/STAT3 Signaling in the Orbitofrontal Cortex.
Authors
JournalNeuropsychopharmacology
PubMed ID27748739
Haploinsufficiency of the schizophrenia and autism risk gene Cyfip1 causes abnormal postnatal hippocampal neurogenesis through microglial and Arp2/3 mediated actin dependent mechanisms.
Authors
JournalTransl Psychiatry
PubMed ID34031371
Energy expenditure during cell spreading influences the cellular response to matrix stiffness.
Authors
JournalBiomaterials
PubMed ID33130323
3D microniches reveal the importance of cell size and shape.