Low-molecular-weight dextran derivatives (f-CMDB) enter the nucleus and are better cell-growth inhibitors compared with parent CMDB polymers.
AuthorsBittoun P,Avramoglou T,Vassy J,Crépin M,Chaubet F,Fermandjian S
JournalCarbohydrate research
PubMed ID10637987
Interaction of antibodies with liposomes bearing fluorescent haptens.
AuthorsPetrossian A, Owicki JC
JournalBiochim Biophys Acta
PubMed ID6477908
'Kinetic and equilibrium aspects of the recognition of antigenic model membranes by antibodies have been studied. Monoclonal anti-fluorescein IgG and its monovalent Fab fragment were allowed to interact with a fluorescein-lipid hapten that was incorporated into phospholipid vesicles. The binding was assayed in the nanomolar hapten concentration range by monitoring ... More
Sites of tubulin polymerization in PC12 cells.
AuthorsKeith CH, Blane K
JournalJ Neurochem
PubMed ID2179472
'The site at which tubulin enters into polymer in the neuritic process is a very important datum in terms of our understanding of the mechanism of transport of the microtubular cytoskeleton out the axon. If the form of tubulin being transported out the axon is the microtubule, then assembly of ... More
DNA strongly impairs the inhibition of cathepsin G by alpha(1)-antichymotrypsin and alpha(1)-proteinase inhibitor.
'This paper explores the possibility that neutrophil-derived DNA interferes with the inhibition of neutrophil cathepsin G (cat G) and proteinase 3 by the lung antiproteinases alpha(1)-proteinase inhibitor (alpha(1)PI), alpha(1)-antichymotrypsin (ACT), and mucus proteinase inhibitor (MPI). A 30-base pair DNA fragment ((30bp)DNA), used as a model of DNA, tightly binds cat ... More
Structural and functional characterization of band 3 from Southeast Asian ovalocytes.
AuthorsMoriyama R, Ideguchi H, Lombardo CR, Van Dort HM, Low PS
JournalJ Biol Chem
PubMed ID1464593
'To determine why deletion of the nine amino acids joining the membrane and cytoplasmic domains of band 3 from Southeast Asian ovalocytes (SAO) renders the erythrocytes rigid, we compared the structural and functional properties of SAO and normal band 3. Calorimetric data, inhibitor binding studies, and anion transport assays all ... More
The organization of F-actin and microtubules in growth cones exposed to a brain-derived collapsing factor.
AuthorsFan J, Mansfield SG, Redmond T, Gordon-Weeks PR, Raper JA
JournalJ Cell Biol
PubMed ID8491778
'In previous work we characterized a brain derived collapsing factor that induces the collapse of dorsal root ganglion growth cones in culture (Raper and Kapfhammer, 1990). To determine how the growth cone cytoskeleton is rearranged during collapse, we have compared the distributions of F-actin and microtubules in normal and partially ... More
Transient alterations in the lateral mobility of erythrocyte membrane components during Sendai virus-mediated fusion.
AuthorsAroeti B, Gutman O, Henis YI
JournalJ Biol Chem
PubMed ID1320013
'Destabilization of the target membrane structure by fusion-promoting viral glycoproteins is assumed to be an essential part of the fusion mechanism. To explore this possibility, we employed fluorescence photobleaching recovery to investigate changes in the lateral mobility of native membrane constituents in human red blood cells (RBCs) during the course ... More
Redistribution of plasma-membrane surface molecules during formation of the Leishmania amazonensis-containing parasitophorous vacuole.
AuthorsHenriques C, de Souza W
JournalParasitol Res
PubMed ID10726992
'Leishmania amazonensis presents two developmental stages that gain access to the host macrophage through phagocytosis. The protozoan resides in a membrane-bound compartment, the parasitophorous vacuole (PV), which can fuse with the endocytic system. For evaluation of the parasite/host-cell interaction process and of PV biogenesis, the two parasite forms or host-cell ... More
Physicochemical characterisation of cationic polybutylcyanoacrylate-nanoparticles by fluorescence correlation spectroscopy.
AuthorsWeyermann J, Lochmann D, Georgens C, Rais I, Kreuter J, Karas M, Wolkenhauer M, Zimmer A,
JournalEur J Pharm Biopharm
PubMed ID15207534
'The aim of this study was to compare different physical and chemical methods with fluorescence correlation spectroscopy (FCS) in order to characterise cationic acrylate nanoparticles (NP), which can deliver oligonucleotides (ON) into mammalian cells. These positively charged nanoparticles were prepared from diethylaminoethyl dextran (DEAE-dextran) and poly(n-butyl-2-cyanoacrylate) (PBCA). NP consists of ... More
Transport of fluorescent phospholipid analogues from the erythrocyte membrane to the parasite in Plasmodium falciparum-infected cells.
AuthorsHaldar K, de Amorim AF, Cross GA
JournalJ Cell Biol
PubMed ID2661561
'The asexual development of the human malaria parasite Plasmodium falciparum is largely intraerythrocytic. When 1-palmitoyl-2-[6-[(7-nitro-2-1,3-benzoxadiazole-4-yl)amino]caproyl] phosphatidylcholine (NBD-PC) was incorporated into infected and uninfected erythrocyte membranes at 0 degrees C, it remained at the cell surface. At 10 degrees C, the lipid was rapidly internalized in infected erythrocytes at all stages ... More
Dynamics of a fluorescent calmodulin analog in the mammalian mitotic spindle at metaphase.
AuthorsStemple DL, Sweet SC, Welsh MJ, McIntosh JR
JournalCell Motil Cytoskeleton
PubMed ID2896549
'We have compared the exchange kinetics of fluorescein-labeled calmodulin and tubulin in the spindles of living mitotic cells at metaphase. Cultured mammalian cells in early stages of mitosis were microinjected with labeled calmodulin or tubulin and returned to an incubator to allow equilibration of the fluorescent protein with the endogenous ... More
A localized pattern photobleaching method for the concurrent analysis of rapid and slow diffusion processes.
AuthorsKoppel DE, Sheetz MP
JournalBiophys J
PubMed ID6616006
'A scanning pattern photobleaching method for the analysis of lateral transport is described and discussed. Fluorescence bleaching with a localized pattern allows for the concurrent analysis of motions over two very different characteristic distances: xi 0(-1), the repeat distance of the pattern, and W, the linear dimension of the illuminated ... More
The movement of fluorescent endocytic tracers in Plasmodium falciparum infected erythrocytes.
AuthorsHaldar K, Uyetake L
JournalMol Biochem Parasitol
PubMed ID1371847
'The fluorescent lipophilic probe 1,1''-dihexadecyl-3-3''-3-3''- tetramethylindocarbocyanine (diIC16) inserted in the red cell surface, functioned as a non-exchangeable lipid marker which was not metabolised or toxic in plasmodial cultures. Invasion by Plasmodium falciparum resulted in the internalisation of the lipid, suggesting the uptake of red cell membrane components during parasite entry. ... More
Lateral mobility of integral proteins in red blood cell tethers.
AuthorsBerk DA, Hochmuth RM
JournalBiophys J
PubMed ID1540701
'The red blood cell membrane is a complex material that exhibits both solid- and liquidlike behavior. It is distinguished from a simple lipid bilayer capsule by its mechanical properties, particularly its shear viscoelastic behavior and by the long-range mobility of integral proteins on the membrane surface. Subject to sufficiently large ... More
Incorporation and turnover of labeled exogenous tubulin in the mitotic spindles of Chaetopterus oocytes and HeLa cells.
AuthorsGoode D, Sarma V
JournalCell Motil Cytoskeleton
PubMed ID3708703
'The incorporation of tubulin into mitotic spindles in situ was studied by incubating permeabilized mitotic cells in solutions containing [3H]GTP-labeled or dichlorotriazinylamino fluorescein (DTAF)-labeled tubulin. Metaphase HeLa cells or spindle-containing "minicells" from Chaetopterus oocytes were lysed in a microtubule-assembly buffer plus 0.5% Nonidet P-40, 1 mg/ml 120,000g supernatant mammalian brain ... More
Uptake and transport of fluorescent derivatives of dolichol in human fibroblasts.
AuthorsJiang LW, Mitchell BA, Teodoro JG, Rip JW
JournalBiochim Biophys Acta
PubMed ID8476914
'We are using fluorescent derivatives to visualize the endocytic transport of dolichol intermediates from the cell surface to the lysosome, and to estimate their rate of turnover within the lysosome. Anthroyl dolichol and anthroyl [1-14C]dolichol were synthesized and purified by chromatography on silica and C18 Sep-Paks followed by high-performance liquid ... More
Detection of ingested bacteria in benthic ciliates using fluorescence in situ hybridization.
AuthorsDiederichs S, Beardsley C, Cleven EJ
JournalSyst Appl Microbiol
PubMed ID14666991
'Fluorescence in situ hybridization (FISH) was applied to detect ingested natural bacteria within the food vacuoles of ciliates harvested from the natural sediment. In addition to this important qualitative aspect, FISH was also successfully used to measure the bacterivory of a culture of the ciliate Tetrahymena pyriformis on natural field ... More
Two-dimensional polyacrylamide gel electrophoresis of proteins labeled with the fluorophore monobromobimane prior to first-dimensional isoelectric focusing: imaging of the fluorescent protein spot patterns using a cooled charge-coupled device.
AuthorsUrwin VE, Jackson P
JournalAnal Biochem
PubMed ID8465962
'A new method for visualizing 2D protein spot patterns is described whereby proteins containing sulfhydryl groups are labeled with the fluorophore monobromobimane prior to the isoelectric focusing step of 2D polyacrylamide gel electrophoresis. The method requires the addition of a single reagent and a delay of only 15 min during ... More
Purification, amino terminal analysis, and peptide mapping of proteins after in situ postelectrophoretic fluorescent labeling.
AuthorsVera JC, Rivas CI, Cortés PA, Cárcamo JO, Delgado J
JournalAnal Biochem
PubMed ID3146233
'Proteins fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis were stained in situ with either 5-(dimethylamino)-1-naphthalene sulfonyl chloride (dansyl chloride) or fluorescein isothiocyanate. This staining procedure can be carried out in less than 30 min without previous fixation of the proteins. It is not dependent on such factors as charge or ... More
Yeast beta-glucan amplifies phagocyte killing of iC3b-opsonized tumor cells via complement receptor 3-Syk-phosphatidylinositol 3-kinase pathway.
AuthorsLi B, Allendorf DJ, Hansen R, Marroquin J, Ding C, Cramer DE, Yan J,
JournalJ Immunol
PubMed ID16849475
'Anti-tumor mAbs hold promise for cancer therapy, but are relatively inefficient. Therefore, there is a need for agents that might amplify the effectiveness of these mAbs. One such agent is beta-glucan, a polysaccharide produced by fungi, yeast, and grains, but not mammalian cells. Beta-glucans are bound by C receptor 3 ... More
Membrane changes in neural target cells studied with fluorescent lectin probes.
AuthorsGualandris L, Rougé P, Duprat AM
JournalJ Embryol Exp Morphol
PubMed ID6655431
'The competent ectoderm of Pleurodeles waltl comprises two cell layers with characteristic differences in their morphology, their composition and the molecular arrangement of the various constituents. The use of labelled lectin probes for observations of ectoderm tissue in vitro with u.v. microscopy (epi-illumination) and the quantification of the results show ... More
Characterisation of corneal fibrotic wound repair at the LASIK flap margin.
AuthorsIvarsen A, Laurberg T, Møller-Pedersen T
JournalBr J Ophthalmol
PubMed ID14507765
'AIM: To characterise temporal changes in corneal wound repair at the LASIK flap margin. METHODS: 18 rabbits received monocular LASIK and were evaluated during 6 months using slit lamp and in vivo confocal microscopy. In three corneas, the exposed stroma was stained with DTAF. At various time points, corneas were ... More
Multiple spectral parameter imaging.
AuthorsWaggoner A, DeBiasio R, Conrad P, Bright GR, Ernst L, Ryan K, Nederlof M, Taylor D
JournalMethods Cell Biol
PubMed ID2648118
Antibody affinity maturation using bacterial surface display.
AuthorsDaugherty PS, Chen G, Olsen MJ, Iverson BL, Georgiou G
JournalProtein Eng
PubMed ID9796833
'A quantitative system for screening combinatorial single-chain Fv (scFv) antibody libraries was developed utilizing surface display on Escherichia coli and fluorescence-activated cell sorting (FACS). This system was employed to isolate clones with high-affinity to a fluorescently-labeled hapten from libraries constructed by randomizing heavy and light-chain residues in the anti-digoxin 26-10 ... More
Preparation, assay, and microinjection of fluorescently labeled cytoskeletal proteins: actin, alpha-actinin, and vinculin.
AuthorsKreis TE
JournalMethods Enzymol
PubMed ID3102901
Fluorescent labeling of cell surfaces.
AuthorsEdidin M
JournalMethods Cell Biol
PubMed ID2464121
Non-radioactive labelling and immunoprecipitation analysis of leukocyte surface proteins using different methods of protein biotinylation.
AuthorsKähne T, Ansorge S
JournalJ Immunol Methods
PubMed ID8308295
'The biotinylation of surface proteins and the detection of immunoprecipitated protein(s) after transfer to nitrocellulose using chemiluminescence methods is a highly sensitive alternative to hazardous radioactive labelling procedures. Ligation of proteins using the common biotin-NHS-ester (N-hydroxysuccinimido-biotin) is often associated with a decrease in immunoreactivity. Here a new non-radioactive method of ... More
Connective tissue remodeling in corneal and scleral wounds.
AuthorsDavison PF, Galbavy EJ
JournalInvest Ophthalmol Vis Sci
PubMed ID3759366
'The fluorescent dye dichlorotriazinyl aminofluorescein will bind to amino groups of proteins covalently under physiological conditions. It has been used to dye the connective tissue around an ulcer or nonpenetrating, linear incision in the rabbit cornea and sclera, and the healing of the tissue has been examined up to 1 ... More
Fluorescent microtubules break up under illumination.
AuthorsVigers GP, Coue M, McIntosh JR
JournalJ Cell Biol
PubMed ID3417772
'We have synthesized three new fluorescent analogues of tubulin, using fluorescein or rhodamine groups attached to N-hydroxy-succinimidyl esters, and have partially characterized the properties of these analogues. We have also further characterized the tubulin derivatized with dichlorotriazinyl-aminofluorescein that has previously been used in this and other laboratories. Our results show ... More
A-CAM: a 135-kD receptor of intercellular adherens junctions. II. Antibody-mediated modulation of junction formation.
AuthorsVolk T, Geiger B
JournalJ Cell Biol
PubMed ID3095334
'Intercellular adherens junctions between cultured lens epithelial cells are highly Ca2+-dependent and are readily dissociated upon chelation of extracellular Ca2+ ions. Addition of Ca2+ to EGTA-treated cells results in the recovery of cell-cell junctions including the reorganization of adherens junction-specific cell adhesion molecule (A-CAM), vinculin, and actin (Volk, T., and ... More
Dichlorotriazinyl aminofluorescein inhibits human keratocyte proliferation in vitro.
AuthorsRofougaran R, Pakkar A, Wee WR, McDonnell PJ
JournalJ Refract Surg
PubMed ID9352485
'PURPOSE: Dichlorotriazinyl aminofluorescein (DTAF) has been used to stain corneal stromal collagen as part of in vivo experimentation. Toxicity of this drug, if present, might alter the observed wound healing. To determine if this drug has any deleterious effect on keratocytes, we evaluated it in vitro. METHODS: Human keratocytes in ... More
Labeling of the glycoprotein subunit of (Na,K)ATPase with fluorescent probes.
AuthorsLee JA, Fortes PA
JournalBiochemistry
PubMed ID2983755
'Sodium plus potassium activated adenosinetriphosphatase [(Na,K)ATPase] is composed of a catalytic subunit (alpha) and a glycoprotein subunit (beta) of unknown function. A method has been developed to label the beta subunit of purified dog kidney (Na,K)ATPase with fluorescent probes. The method consists of oxidation of beta-subunit oligosaccharides, reaction of the ... More
Polymerization of tubulin in vivo: direct evidence for assembly onto microtubule ends and from centrosomes.
AuthorsSoltys BJ, Borisy GG
JournalJ Cell Biol
PubMed ID3886672
'Microtubule assembly in vivo was studied by hapten-mediated immunocytochemistry. Tubulin was derivatized with dichlorotriazinylaminofluorescein (DTAF) and microinjected into living, interphase mammalian cells. Sites of incorporation were determined at the level of individual microtubules by double-label immunofluorescence. The haptenized tubulin was localized by an anti-fluorescein antibody and a second antibody conjugated ... More
A comparative study of the distribution of fluorescently labeled calmodulin and tubulin in the meiotic apparatus of the mouse oocyte.
AuthorsHamaguchi Y, Iwasa F, Toriyama M, Sakai H
JournalCell Struct Funct
PubMed ID2743423
'The localizations of tubulin and calmodulin were investigated in the mouse oocyte during the second meiosis by fluorescently labeling and microinjecting these proteins prepared from porcine brain tissue. When injected, both tubulin and calmodulin were quickly incorporated into the preformed meiotic apparatus of the oocyte at metaphase. The localization of ... More
Synthesis and characterization of highly sensitive heparin probes for detection of heparin-binding proteins.
'Three labeled heparin species were synthesized as probes for heparin-binding protein detection. Heparin conjugated with 5([4,6-dichlorotriazin-2-yl]amino)fluorescein can be iodinated to a high specific activity. This probe specifically detected 40 pg histone on a dot blot without affinity purification. Heparin biotinylated on its naturally occurring primary amino groups also detected known ... More
Caspase cleaved BID targets mitochondria and is required for cytochrome c release, while BCL-XL prevents this release but not tumor necrosis factor-R1/Fas death.
AuthorsGross A, Yin XM, Wang K, Wei MC, Jockel J, Milliman C, Erdjument-Bromage H, Tempst P, Korsmeyer SJ
JournalJ Biol Chem
PubMed ID9873064
'"BH3 domain only" members of the BCL-2 family including the pro-apoptotic molecule BID represent candidates to connect with proximal signal transduction. Tumor necrosis factor alpha (TNFalpha) treatment induced a caspase-mediated cleavage of cytosolic, inactive p22 BID at internal Asp sites to yield a major p15 and minor p13 and p11 ... More
Fluorescent dyes demonstrate the uniform expansion of the growing rabbit cornea.
AuthorsDavison PF, Galbavy EJ
JournalInvest Ophthalmol Vis Sci
PubMed ID4030248
'Thiocyanate and triazinyl chloride derivatives of fluorescent dyes have been employed for the covalent labeling of components of the connective tissue of the rabbit cornea. Collagen is the major macromolecular component that becomes dyed. Some of the stromal components that are labeled by these reagents in the first two weeks ... More
Fluorescent staining of proteins transferred to nitrocellulose allowing for subsequent probing with antisera.
AuthorsSzewczyk B, Summers DF
JournalAnal Biochem
PubMed ID2445223
'A sensitive staining method for protein blots on nitrocellulose is described. It is based on the coupling of a fluorochrome, dichlorotriazynylaminofluorescein, to protein which yields products colorless in visible light but colored when protein blots are illuminated with long-range ultraviolet light. The coupling of a fluorochrome does not affect the ... More
A disintegration method for direct counting of bacteria in clay-dominated sediments: dissolving silicates and subsequent fluorescent staining of bacteria.
AuthorsBoenigk J
JournalJ Microbiol Methods
PubMed ID14744444
'The masking of bacteria by abundant microparticles of the clay and silt fraction and cell losses due to sonication hampered direct enumeration of bacteria in sediments dominated by fine sediments. These problems can be circumvented by dissolving silicate fine particles using hydrofluoric acid and subsequent staining of bacteria by DTAF. ... More
Immunofluorescence using dichlorotriazinylaminofluorescein (DTAF). I. Preparation and fractionation of labelled IgG.
AuthorsBlakeslee D, Baines MG
JournalJ Immunol Methods
PubMed ID13125
'Dichlorotriazinylaminofluorescein (DTAF), the product of the reaction of aminofluorescein with cyanuric chloride, is an effective reagent for conjugating fluorescein to immunnoglobulins. DTAF has absorption and emission properties nearly identical to fluoresceinisothiocyanate (FITC) and DTAF and FITC-labelled antibodies are similar in terms of preparation and specificity of immlnofluorescence. However, DTAF is ... More
Chromosomes move poleward in anaphase along stationary microtubules that coordinately disassemble from their kinetochore ends.
AuthorsGorbsky GJ, Sammak PJ, Borisy GG
JournalJ Cell Biol
PubMed ID3793763
'During the movement of chromosomes in anaphase, microtubules that extend between the kinetochores and the poles shorten. We sought to determine where subunits are lost from these microtubules during their shortening. Prophase or prometaphase cells on coverslips were injected with fluoresceinated tubulin and allowed to progress through mitosis. Immediately after ... More
Quantitative and selective fluorophore labeling of phosphoserine on peptides and proteins: characterization at the attomole level by capillary electrophoresis and laser-induced fluorescence.
AuthorsFadden P, Haystead TA
JournalAnal Biochem
PubMed ID7539987
'Reaction conditions were defined for the selective quantitative derivatization and fluorophore labeling of phosphoserine residues on peptides and proteins. Phosphoserine was derivatized with 1,2-ethanedithiol using a modification of the reaction conditions defined by R. C. Clark and J. Dijkstra (1967) Int. J. Biochem. 11, 577-585 and H. E. Meyer, E. ... More
Crosslinkage and visualization of acetylcholine receptors on myotubes with biotinylated alpha-bungarotoxin and fluorescent avidin.
AuthorsAxelrod D
JournalProc Natl Acad Sci U S A
PubMed ID6933533
'A biotinylated derivative of alpha-bungarotoxin and tetramethylrhodamine-labeled avidin were employed to fluorescence label the acetylcholine receptors (AcChoR) on the surface of rat myotubes in primary culture. Because of the multivalency of both the biotinylated bungarotoxin and the avidin, this treatment extensivey crosslinks the AcChoR. AcChoR crosslinking immobilizes more than 90% ... More
Kinetics of activation of human prothrombin. Use of a fluorescein-labeled derivative to obtain kinetic constants as a function of factor V concentration and activation state.
AuthorsMorrison SA
JournalBiochemistry
PubMed ID6615817
Co-clustering and internalization of low-density lipoproteins and alpha 2-macroglobulin in human skin fibroblasts.
AuthorsVia DP, Willingham MC, Pastan I, Gotto AM, Smith LC
JournalExp Cell Res
PubMed ID6180918
Fluorescence photobleaching does not alter the lateral mobility of erythrocyte membrane glycoproteins.
AuthorsKoppel DE, Sheetz MP
JournalNature
PubMed ID7266669
Preparation and characterization of fluorescent analogs of tubulin.
AuthorsWadsworth P, Salmon ED
JournalMethods Enzymol
PubMed ID3821576
Preparation of a fluorescent analog: acetamidofluoresceinyl-labeled Dictyostelium discoideum alpha-actinin.
AuthorsSimon JR, Taylor DL
JournalMethods Enzymol
PubMed ID3029546
Fluorescence microphotolysis. Diffusion measurements in single cells.
AuthorsPeters R
JournalNaturwissenschaften
PubMed ID6877390
Fluorescent localization of contractile proteins in tissue culture cells.
AuthorsWang K, Feramisco JR, Ash JF
JournalMethods Enzymol
PubMed ID6750319
Dichlorotriazineylaminofluorescein--a new fluorochrome for cytochemical and histochemical detection of proteins
AuthorsBarskii VE, Ivanov VB, Skliar IuE, Mikhailov GI,
JournalIzv Akad Nauk SSSR Biol
PubMed ID5739155
No abstract available
Immunofluorescence using dichlorotriazinylaminofluorescein (DTAF). II. Preparation, purity and stability of the compound.
AuthorsBlakeslee D
JournalJ Immunol Methods
PubMed ID334995
Dichlorotriazinylaminofluorescein (DTAF) is quickly and easily prepared under anhydrous conditions in high yield and purity. DTAF stored desiccated at 4 degrees C for over a year undergoes about 1% hydrolysis but with no loss of its protein-labelling efficiency. ... More
Preparation and properties of fluorescein-labelled hyaluronate.
Authorsde Belder AN, Wik KO
JournalCarbohydr Res
PubMed ID1203905
Hyaluronate has been labelled with fluorescein groups by two procedures. Products with degrees of substitution ((d.s.) between 0.05 and 0.001 were obtained. Physico-chemical analysis (viscometry, gel chromatography, and measurements of sedimentation and diffusion coefficients) of the parent compound and the products showed that the labelling procedures caused only a limited ... More
Synthesis of a biologically active fluorescent muramyl dipeptide congener.
AuthorsHiebert CK, Kopp WC, Richerson HB, Barfknecht CF
JournalJ Med Chem
PubMed ID6644742
A fluorescent-labeled muramyl dipeptide (MDP) has been prepared to probe immunoadjuvant cellular interactions. N-Acetylmuramyl-L-alanyl-D-isoglutamine (1) was synthesized in improved yield and reacted with 2-(fluoresceinylamino)-4,6-dichloro-s-triazine (DTAF, 2) to give the fluorescent adduct DTAF-MDP (3), attached through the 6-position of the sugar moiety. Adjuvant activity was assessed by using two different in ... More
Halistaurin, phialidin and modified forms of aequorin as Ca2+ indicator in biological systems.
AuthorsShimomura O, Shimomura A
JournalBiochem J
PubMed ID4026808
Two kinds of aequorin-type photoproteins, i.e., halistaurin and phialidin, and four kinds of modified forms of aequorin, i.e., products of acetylation, ethoxycarbonylation, fluorescamine-modification and fluorescein labelling, were prepared. The modified forms of aequorin were more sensitive to Ca2+ than was aequorin in their Ca2+-triggered luminescence reactions, whereas halistaurin and phialidin ... More
Simultaneous strand displacement amplification and fluorescence polarization detection of Chlamydia trachomatis DNA.
AuthorsSpears PA, Linn CP, Woodard DL, Walker GT
JournalAnal Biochem
PubMed ID9126382
Strand displacement amplification (SDA) is an isothermal DNA amplification technology that uses a restriction enzyme and polymerase. We have developed a target-specific method which allows simultaneous SDA and detection in a homogeneous format. This is accomplished by including a detector oligodeoxynucleotide labeled with 5-(4,6-dichlorotriazin-2-yl)amino fluorescein in the SDA reaction. Fluorescence ... More
Direct visualization of fluorescein-labeled microtubules in vitro and in microinjected fibroblasts.
AuthorsKeith CH, Feramisco JR, Shelanski M
JournalJ Cell Biol
PubMed ID7193677
Microtubule proteins and tubulin have been purified from brain and labeled with dichlorotriazinyl fluorescein (DTAF). This procedure compromises neither the polymerizability of the proteins nor their affinities for unlabeled proteins. Within 15 min after microinjection of either DTAF-microtubule proteins or DTAF-tubulin into cultured gerbil fibroma cells, there was an evolution ... More
Slow transport of tubulin in the neurites of differentiated PC12 cells.
AuthorsKeith CH
JournalScience
PubMed ID2432662
In order to study the rate and form of tubulin transport in cultured neuronal cells, the fluorescence recovery after the photobleaching of a fluorescent tubulin analog has been followed within the neuritic processes of differentiated PC12 cells. In these cells, as in peripheral axons, tubulin is transported in coherent, nondiffusing ... More
Comparison of three different activation methods for coupling antibodies to magnetisable cellulose particles.
Authorsal-Abdulla IH, Mellor GW, Childerstone MS, Sidki AM, Smith DS
JournalJ Immunol Methods
PubMed ID2551968
The periodate and 1,1'-carbonyldiimidazole activation methods were compared with the cyanogen bromide procedure for coupling antibodies to magnetisable cellulose/iron oxide solid-phase particles. Fluoroimmunoassays for quinine, primaquine, normetanephrine and cannabinoids were employed to assess the binding properties of such coupled solid phases. The cyanogen bromide and 1,1'-carbonyldiimidazole methods gave similar products ... More
An ultrasensitive, continuous fluorometric assay for calpain activity.
AuthorsTompa P, Schád E, Baki A, Alexa A, Batke J, Friedrich P
JournalAnal Biochem
PubMed ID8572308
A rapid, continuous assay for calcium-activated neutral protease activity is described. This assay is based on monitoring the elevation in fluorescence intensity that occurs upon calpainolytic digestion of dichlorotriazinylamino-fluorescein-labeled microtubule-associated protein 2. Tedious separation of peptide products from the protein substrate in this rapid assay is unnecessary, which thus offers ... More
Synthesis and characterization of a highly fluorescent peptidyl-phosphatidylethanolamine.
AuthorsPetrossian A, Kantor AB, Owicki JC
JournalJ Lipid Res
PubMed ID4031655
The synthesis of a fluorescent lipid for use in studies of immune recognition of model membranes is described. The molecule has the basic structure HAPTEN-SPACER-LIPID, where fluorescein is the hapten, an oligopeptide (triglycine) is the spacer, and dipalmitoylphosphatidylethanolamine (DPPE) is the lipid. The spacer, which is necessary for immunological reactivity, ... More
Measurement of local strains in intervertebral disc anulus fibrosus tissue under dynamic shear: contributions of matrix fiber orientation and elastin content.
AuthorsMichalek AJ, Buckley MR, Bonassar LJ, Cohen I, Iatridis JC,
JournalJ Biomech
PubMed ID19664773
Shear strain has been implicated as an initiator of intervertebral disc anulus failure, however a clear, multi-scale picture of how shear strain affects the tissue microstructure has been lacking. The purposes of this study were to measure microscale deformations in anulus tissue under dynamic shear in two orientations, and to ... More
Synthesis and biological evaluation of the superagonist [N alpha-chlorotriazinylaminofluorescein-Ser1,Nle4,D-Phe7]-al pha-MSH.
AuthorsChaturvedi DN, Hruby VJ, Castrucci AM, Kreutzfeld KL, Hadley ME
JournalJ Pharm Sci
PubMed ID2989482
The fluorescein-labeled melanotropin [N alpha-chlorotriazinylaminofluorescein-Ser1,Nle4,D-Phe 7]-alpha-MSH, was prepared by solid-phase techniques of peptide synthesis. The biological actions of this analogue were determined in several melanocyte bioassays and were compared with the parent peptide [Nle4,D-Phe7]-alpha-MSH and the native hormone alpha-MSH. The fluorescein compound was a superpotent agonist with approximately 10 times ... More
Fluorescence labeling and quantification of oxygen-containing functionalities on the surface of single-walled carbon nanotubes.
AuthorsDementev N, Feng X, Borguet E,
JournalLangmuir
PubMed ID19563231
Fluorescence labeling of surface species (FLOSS) was applied to identify and determine the concentration of oxygen-containing functionalities on single-walled carbon nanotubes (SWCNTs), subjected to two different purification processes (air/HCl and nitric acid treatments) and compared to as-received (nonpurified) SWCNTs. The fluorophores were selected for their ability to covalently bind, with ... More
Fluorescence polarization immunoassay. I. Monitoring aminoglycoside antibiotics in serum and plasma.
AuthorsJolley ME, Stroupe SD, Wang CH, Panas HN, Keegan CL, Schmidt RL, Schwenzer KS
JournalClin Chem
PubMed ID7016372
Fluorescence polarization immunoassays of the aminoglycoside antibiotics gentamicin, tobramycin, and amikacin in plasma and serum are described and shown to be clinically useful. The aminoglycoside tracers were prepared by reacting the parent compounds with 5-[(4,6-dichlorotriazin-2-yl)-amino] fluorescein. Antisera specific for the compounds were raised in rabbits by conventional procedures. Tracer, sample, ... More
Distinct subsets of somatostatin receptors on cultured human lymphocytes.
AuthorsSreedharan SP, Kodama KT, Peterson KE, Goetzl EJ
JournalJ Biol Chem
PubMed ID2562957
Somatostatin (SOM) is a neuroendocrine tetradecapeptide that suppresses specific functions of differentiated T-cells and antibody-producing cells. The Jurkat line of human leukemic T-cells and U266 IgE-producing human myeloma cells bound [I-Tyr11]SOM specifically. The maximal level of specific binding was attained by 1-2 h at 22 degrees C for both types ... More
Preparation and tumor cell uptake of poly(N-isopropylacrylamide) folate conjugates.
AuthorsDubé D, Francis M, Leroux JC, Winnik FM
JournalBioconjug Chem
PubMed ID12009963
Folate conjugates (PNIPAM-NH-FA) of a copolymer of N-isopropylacrylamide (NIPAM) and amino-N'-ethylenedioxy-bis(ethylacrylamide) were prepared by an efficient synthesis leading to random grafting, via a short dioxyethylene spacer, of approximately 7 folic acid residues per macromolecule. The chemical composition of the copolymer was characterized by (1)H NMR and UV/vis spectroscopy. A fluorophore-labeled ... More
Behaviour of microtubules and actin filaments in living Drosophila embryos.
AuthorsKellogg DR, Mitchison TJ, Alberts BM
JournalDevelopment
PubMed ID3248521
We describe the preparation of novel fluorescent derivatives of rabbit muscle actin and bovine tubulin, and the use of these derivatives to study the behaviour of actin filaments and microtubules in living Drosophila embryos, in which the nuclei divide at intervals of 8 to 21 min. The fluorescently labelled proteins ... More
Major histocompatibility complex independent T cell receptor-antigen interaction: functional analysis using fluorescein derivatives.
We have isolated T cell receptor (TCR) cDNAs from fluorescein (FL)-specific human T cell clones (alpha FL beta FL), and transferred them to TCR beta- Jurkat cells in order to study direct FL-binding to the TCR. Using either FL-conjugated polymers (FL-polymer) or FL-substituted Sepharose beads, we are able to demonstrate ... More
The synthesis of fluorescent chlorotriazinylaminofluorescein-concanavalin A and its use as a glycoprotein stain on sodium dodecyl sulphate/polyacrylamide gels.
AuthorsMahoney CW, Azzi A
JournalBiochem J
PubMed ID3632634
Concanavalin A, a specific glycoprotein probe, was optimally labelled to a maximum stoichiometry of 0.4 mol of chlorotriazinylaminofluorescein (CTAF)/mol of concanavalin A monomer under mild reaction conditions (pH 8.0, 6 h), and under these conditions the CTAF concanavalin A preparation retains its carbohydrate-binding ability and is able to penetrate SDS/7.5-15%-polyacrylamide ... More
Site-directed labeling of a monoclonal antibody: targeting to a disulfide bond.
AuthorsPackard B, Edidin M, Komoriya A
JournalBiochemistry
PubMed ID3718943
We have designed and synthesized crabescein, the first member of a class of fluorescent labels that add across disulfide bonds. Crabescein is a fluorescein derivative that reports the rotational correlation time of the immunoglobulin G (IgG) segment to which it is covalently bound. Chemical analysis of the IgG labeled with ... More
Measurement of specific protease activity utilizing fluorescence polarization.
AuthorsLevine LM, Michener ML, Toth MV, Holwerda BC
JournalAnal Biochem
PubMed ID9126375
A fluorescence polarization assay was designed to measure proteolytic cleavage of a specific peptide substrate for human cytomegalovirus protease. The peptide substrate was derivatized by biotinylation of a gamma-aminobutyric acid-modified amino-terminus and labeled with 5-(4,6-dichlorotriazinyl)aminofluorescein at the carboxy-terminus. Incubation of this substrate with recombinant human cytomegalovirus protease and subsequent addition ... More
Redistribution of a major cell surface glycoprotein during cell movement.
AuthorsJacobson K, O'Dell D, Holifield B, Murphy TL, August JT
JournalJ Cell Biol
PubMed ID6386823
The distribution in living cells of an 80,000-dalton major cell surface glycoprotein of murine fibroblasts has been studied by use of monoclonal antibodies. The presence of the molecule throughout the plasma membrane and on the substrate attached surface of the cell was demonstrated by immunofluorescence. Cell growth kinetics were not ... More
Location and dynamics of a membrane-bound fluorescent hapten. A spectroscopic study.
AuthorsStanton SG, Kantor AB, Petrossian A, Owicki JC
JournalBiochim Biophys Acta
PubMed ID6477909
In the preceding paper (Petrossian, A. and Owicki, J.C. (1984), Biochim. Biophys. Acta 776, 217-227), we describe the binding of a monoclonal anti-fluorescein antibody to a membrane bound fluorescein-lipid hapten. Those results suggest that some of the hapten fluorescein moiety is extended away from the membrane surface and is available ... More
Thyrotropin-releasing hormone increases cytosolic free Ca2+ in clonal pituitary cells (GH3 cells): direct evidence for the mobilization of cellular calcium.
AuthorsSchlegel W, Wollheim CB
JournalJ Cell Biol
PubMed ID6429159
Changes in the cytosolic free Ca2+ concentration following cell surface receptor activation have been proposed to mediate a wide variety of cellular responses. Using the specific Ca2+ chelator quin2 as a fluorescent intracellular probe, we measured the Ca2+ levels in the cytosol of clonal rat pituitary cells, GH3 cells. We ... More
In-capillary derivatization and analysis of amino acids, amino phosphonic acid-herbicides and biogenic amines by capillary electrophoresis with laser-induced fluorescence detection.
AuthorsMolina M, Silva M
JournalElectrophoresis
PubMed ID12210240
This paper describes a general approach for the in-capillary derivatization of amino compounds and the subsequent sensitive determination of the derivatives by micellar electrokinetic chromatography (MEKC) or capillary zone electrophoresis (CZE) with laser-induced fluorescence (LIF) detection. Amino acids, biogenic amines and amino phosphonic acid-herbicides were chosen as model analytes to ... More
Fluorescent and radiolabeling of polysaccharides: binding and internalization experiments on vascular cells.
AuthorsPrigent-Richard S, Cansell M, Vassy J, Viron A, Puvion E, Jozefonvicz J, Letourneur D
JournalJ Biomed Mater Res
PubMed ID9549622
Glycosaminoglycans (GAGs) such as heparan sulfates are complex carbohydrate polymers. These structural components of the extracellular matrix are essential for the adhesion, migration, and regulation of cellular growth. To understand the physiological role of GAGs and GAG analogues, a practical approach consists of labeling and detecting them in cell extracts, ... More
Origin of a fluorescence increase accompanying the limited proteolysis of fluorescein-labeled human prothrombin by Factor Xa.
AuthorsMorrison SA
JournalInt J Pept Protein Res
PubMed ID6439669
In a search for a probe which would report its proteolysis to thrombin, the human blood coagulation zymogen prothrombin was covalently labeled with fluorescein. Fluorescein isothiocyanate (FITC) and dichlorotriazinylaminofluorescein (DCTAF) both introduced approximately 1 molecule of dye, but labeling occurred at different locations, as FITC had no effect on clotting ... More
A multiple high-resolution mini two-dimensional polyacrylamide gel electrophoresis system: imaging two-dimensional gels using a cooled charge-coupled device after staining with silver or labeling with fluorophore.
AuthorsUrwin VE, Jackson P
JournalAnal Biochem
PubMed ID1716069
A multiple mini two-dimensional electrophoretic method which results in three two-dimensional protein spot patterns being positioned side by side in an individual gel has been developed. Preparation time has been minimized by employing disposable capillary tubes for the isoelectric focusing gels and reducing the number of second-dimensional gels required. Commercially ... More
Junctional adhesion molecule interacts with the PDZ domain-containing proteins AF-6 and ZO-1.
AuthorsEbnet K, Schulz CU, Meyer Zu Brickwedde MK, Pendl GG, Vestweber D
JournalJ Biol Chem
PubMed ID10856295
We have identified the PDZ domain protein AF-6 as an intracellular binding partner of the junctional adhesion molecule (JAM), an integral membrane protein located at cell contacts. Binding of AF-6 to JAM required the presence of the intact C terminus of JAM, which represents a classical type II PDZ domain-binding ... More
Fluorescent cellulose microfibrils as substrate for the detection of cellulase activity.
AuthorsHelbert W, Chanzy H, Husum TL, Schülein M, Ernst S
JournalBiomacromolecules
PubMed ID12741760
To devise a sensitive cellulase assay based on substrates having most of the physical characteristics of native cellulose, 5-(4,6-dichlorotriazinyl)aminofluorescein (DTAF) was used as a grafting agent to prepare suspensions of fluorescent microfibrils from bacterial cellulose. These suspensions were digested by a series of commercially relevant cellulases from Humicola insolens origin: ... More
A fluorescence-based one-step assay for serum non-transferrin-bound iron.
AuthorsBreuer W, Cabantchik ZI
JournalAnal Biochem
PubMed ID11730343
We introduce a method for monitoring non-transferrin-bound iron (NTBI), a labile and potentially toxic form of serum iron associated with imbalanced iron metabolism. The assay employs fluorescein-labeled apotransferrin (Fl-aTf), which undergoes fluorescence quenching upon binding iron. It has the advantages of simplicity, high sensitivity, and detection of those forms of ... More
Tubulin dynamics in cultured mammalian cells.
AuthorsSaxton WM, Stemple DL, Leslie RJ, Salmon ED, Zavortink M, McIntosh JR
JournalJ Cell Biol
PubMed ID6501419
Bovine neurotubulin has been labeled with dichlorotriazinyl-aminofluorescein (DTAF-tubulin) and microinjected into cultured mammalian cells strains PTK1 and BSC. The fibrous, fluorescence patterns that developed in the microinjected cells were almost indistinguishable from the pattern of microtubules seen in the same cells by indirect immunofluorescence. DTAF-tubulin participated in the formation of ... More
The identification of extracellular matrix (ECM) binding sites on the basal surface of embryonic corneal epithelium and the effect of ECM binding on epithelial collagen production.
AuthorsSugrue SP, Hay ED
JournalJ Cell Biol
PubMed ID3517010
Previously, we have shown that embryonic corneal epithelia can interact with, and respond to, soluble extracellular matrices (ECM) (laminin, collagen, and fibronectin). The basal surface of epithelia isolated free of the underlying ECM can be seen to be disrupted by numerous blebs that sprout from this formerly smooth surface. Laminin, ... More
Requirement of ryanodine receptor subtypes 1 and 2 for Ca(2+)-induced Ca(2+) release in vascular myocytes.
AuthorsCoussin F, Macrez N, Morel JL, Mironneau J
JournalJ Biol Chem
PubMed ID10734110
While the roles of subtypes 1 and 2 of the ryanodine receptors (RYRs) have been studied in cellular systems expressing specifically one or the other of these subtypes (i.e. skeletal and cardiac muscle), the function of these receptors has not been evaluated in smooth muscles. We have previously reported RYR-mediated ... More
Noninvasive measurement of the pH of the endoplasmic reticulum at rest and during calcium release.
AuthorsKim JH, Johannes L, Goud B, Antony C, Lingwood CA, Daneman R, Grinstein S
JournalProc Natl Acad Sci U S A
PubMed ID9501204
The pH within individual organelles of the secretory pathway is believed to be an important determinant of their biosynthetic activity. However, little is known about the determinants and regulation of the pH in the secretory organelles, which cannot be readily accessed by [H+]-sensitive probes. We devised a procedure for the ... More
Assessment of fluorescent-labeled bacteria for evaluation of in vivo uptake of bacteria (Vibrio spp.) by crustacean larvae.
AuthorsSoto-Rodriguez SA, Simões N, Jones DA, Roque A, Gomez-Gil B
JournalJ Microbiol Methods
PubMed ID12401232
Available methods to study crustacean digestive tract colonization by bacteria are laborious, time-consuming, and do not permit in vivo assays and observation. This paper reports on a rapid and consistent technique to apply a fluorescent label to bacteria, which can then be presented to filter-feeding crustacea such as Artemia and ... More
The use of confocal laser scanning microscopy to analyze the process of parasitic protozoon-host cell interaction.
Authorsde-Souza W, de-Carvalho TU, de-Melo ET, Soares CP, Coimbra ES, Rosestolato CT, Ferreira SR, Vieira M
JournalBraz J Med Biol Res
PubMed ID9921284
In this communication we review the results obtained with the confocal laser scanning microscope to characterize the interaction of epimastigote and trypomastigote forms of Trypanosoma cruzi and tachyzoites of Toxoplasma gondii with host cells. Early events of the interaction process were studied by the simultaneous localization of sites of protein ... More
Assembly properties of fluorescein-labeled tubulin in vitro before and after fluorescence bleaching.
Brain tubulin has been conjugated with dichlorotriazinyl-aminofluorescein (DTAF) to form a visualizable complex for the study of tubulin dynamics in living cells. By using several assays we confirm the finding of Keith et al. (Keith, C. H., J. R. Feramisco, and M. Shelanski, 1981, J. Cell Biol., 88:234-240) that DTAF-tubulin ... More
Redistribution of parasite and host cell membrane components during Toxoplasma gondii invasion.
AuthorsPacheco-Soares C, De Souza W
JournalCell Struct Funct
PubMed ID9706405
The initial association of tachyzoites of Toxoplasma gondii with a host cell induces an endocytic process which leads to the formation of a vacuole known as the parasitophorous vacuole (PV). We analyzed the parasite-host cell interaction process using either parasites or host cells whose membrane was previously labeled with probes ... More
Modulation of membrane protein lateral mobility by polyphosphates and polyamines.
AuthorsSchindler M, Koppel DE, Sheetz MP
JournalProc Natl Acad Sci U S A
PubMed ID6929496
The lateral mobility of fluorescein-labeled membrane glycoproteins was measured in whole unlysed erythrocytes and erythrocyte ghosts by the technique of "fluorescence redistribution after fusion." Measurements were made on polyethylene glycol-fused cell pairs in which only one member of the couplet was initially fluorescently labeled. Diffusion coefficients were estimated from the ... More
Comparison of three common amine reactive fluorescent probes used for conjugation to biomolecules by capillary zone electrophoresis.
AuthorsBanks PR, Paquette DM
JournalBioconjug Chem
PubMed ID7578365
The conjugation of three amine reactive fluorescent probes, each containing the fluorophore fluorescein but different reactive moieties, was compared using the protein myoglobin and the amino acid L-lysine as reagents. The three different reactive moieties were an isothiocyanate group (FITC), a succinimidyl ester group (CFSE), and a dichlorotriazine group (DTAF). ... More
Molecular heterogeneity of adherens junctions.
AuthorsGeiger B, Volk T, Volberg T
JournalJ Cell Biol
PubMed ID3930512
We describe here the subcellular distributions of three junctional proteins in different adherens-type contacts. The proteins examined include vinculin, talin, and a recently described 135-kD protein (Volk, T., and B. Geiger, 1984, EMBO (Eur. Mol. Biol. Organ.) J., 10:2249-2260). Immunofluorescent localization of the three proteins indicated that while vinculin was ... More
Structure-function studies on small heat shock protein oligomeric assembly and interaction with unfolded polypeptides.
AuthorsLeroux MR, Melki R, Gordon B, Batelier G, Candido EP
JournalJ Biol Chem
PubMed ID9305934
The small heat shock protein (smHSP) and alpha-crystallin genes encode a family of 12-43-kDa proteins which assemble into large multimeric structures, function as chaperones by preventing protein aggregation, and contain a conserved region termed the alpha-crystallin domain. Here we report on the structural and functional characterization of Caenorhabditis elegans HSP16-2, ... More
Protistan grazing analysis by flow cytometry using prey labeled by in vivo expression of fluorescent proteins.
AuthorsFu Y, O'Kelly C, Sieracki M, Distel DL
JournalAppl Environ Microbiol
PubMed ID14602649
Selective grazing by protists can profoundly influence bacterial community structure, and yet direct, quantitative observation of grazing selectivity has been difficult to achieve. In this investigation, flow cytometry was used to study grazing by the marine heterotrophic flagellate Paraphysomonas imperforata on live bacterial cells genetically modified to express the fluorescent ... More
The translational mobility of substances within the cytoplasmic matrix.
AuthorsJacobson K, Wojcieszyn J
JournalProc Natl Acad Sci U S A
PubMed ID6387711
The translational mobility of fluorescent-labeled molecules injected into the cytoplasm of living cells can be measured by the fluorescence recovery after photobleaching (FRAP) technique. In the fibroblast cytoplasm, the diffusion coefficients, D, of test macromolecules ranging in molecular weight from 12,000 to 440,000 are about 10(-8) cm2/sec and exhibit almost ... More
Quaternary ammonium beta-cyclodextrin nanoparticles for enhancing doxorubicin permeability across the in vitro blood-brain barrier.
AuthorsGil ES, Li J, Xiao H, Lowe TL,
JournalBiomacromolecules
PubMed ID19216528
This study describes novel quaternary ammonium beta-cyclodextrin (QAbetaCD) nanoparticles as drug delivery carriers for doxorubicin (DOX), a hydrophobic anticancer drug, across the blood-brain barrier (BBB). QAbetaCD nanoparticles show 65-88 nm hydrodynamic radii with controllable cationic properties by adjusting the incorporated amount of quaternary ammonium group in their structure. ATR-FTIR studies ... More
Fluorescently labeled collagen binding proteins allow specific visualization of collagen in tissues and live cell culture.
AuthorsKrahn KN, Bouten CV, van Tuijl S, van Zandvoort MA, Merkx M
JournalAnal Biochem
PubMed ID16476406
Visualization of the formation and orientation of collagen fibers in tissue engineering experiments is crucial for understanding the factors that determine the mechanical properties of tissues. In this study, collagen-specific fluorescent probes were developed using a new approach that takes advantage of the inherent specificity of collagen binding protein domains ... More
An efficient method for introducing macromolecules into living cells.
AuthorsDoxsey SJ, Sambrook J, Helenius A, White J
JournalJ Cell Biol
PubMed ID2989298
The hemagglutinin (HA) of influenza virus was used to obtain efficient and rapid bulk delivery of antibodies and horseradish peroxidase (HRP) into the cytoplasm of living tissue culture cells. By exploiting HA's efficient cell surface expression, its high affinity for erythrocytes, and its acid-dependent membrane fusion activity, a novel delivery ... More
Latex beads as probes of a neural crest pathway: effects of laminin, collagen, and surface charge on bead translocation.
AuthorsBronner-Fraser M
JournalJ Cell Biol
PubMed ID6373786
In the trunk region of avian embryos, neural crest cells migrate along two pathways: dorsally just under the ectoderm, and ventrally between the neural tube and the somites. Previous work from this laboratory has shown that uncoated latex beads are able to translocate along the ventral neural crest pathway after ... More