JournalJournal of immunology (Baltimore, Md. : 1950)
PubMed ID17548610
Phenotypic and functional maturation of dendritic cells mediated by heparan sulfate.
AuthorsKodaira Y, Nair SK, Wrenshall LE, Gilboa E, Platt JL
JournalJ Immunol
PubMed ID10903769
'Primary immune responses are thought to be induced by dendritic cells. To promote such responses, dendritic cells must be activated by exogenous agonists, such as LPS, or by products of activated leukocytes, such as TNF-alpha and IL-1. How dendritic cells might be activated in the absence of exogenous stimuli, or ... More
An 11-color flow cytometric assay for identifying, phenotyping, and assessing endocytic ability of peripheral blood dendritic cell subsets in a single platform.
AuthorsWang JC, Kobie JJ, Zhang L, Cochran M, Mosmann TR, Ritchlin CT, Quataert SA,
JournalJ Immunol Methods
PubMed ID19049809
Human peripheral blood dendritic cells (PBDC) are a rare population comprised of several distinctive subsets. Analysis of these cells has been hindered by their low frequency. In this study, we report a novel direct ex vivo 11-color flow cytometric assay that combines subset identification with analysis of activation status and ... More
Use of dextran beads for live analysis of the nuclear division and nuclear envelope breakdown/reformation cycles in the Drosophila embryo.
AuthorsKalpin RF, Daily DR, Sullivan W,
JournalBiotechniques
PubMed ID7530458
We employ fluorescently labeled dextran beads to follow cycles of nuclear division and nuclear envelope breakdown in living Drosophila embryos. When injected into syncytial Drosophila embryos, 3000 mol wt fluorescently labeled dextran beads concentrate in the interphase nucleus. Through confocal microscopy, undisrupted multiple cycles of nuclear division are readily followed. ... More
Mammalian reovirus, a nonfusogenic nonenveloped virus, forms size-selective pores in a model membrane.
AuthorsAgosto MA, Ivanovic T, Nibert ML
JournalProc Natl Acad Sci U S A
PubMed ID17053074
During cell entry, reovirus particles with a diameter of 70-80 nm must penetrate the cellular membrane to access the cytoplasm. The mechanism of penetration, without benefit of membrane fusion, is not well characterized for any such nonenveloped animal virus. Lysis of RBCs is an in vitro assay for the membrane ... More
Amino acid sequence requirements of the transmembrane and cytoplasmic domains of influenza virus hemagglutinin for viable membrane fusion.
AuthorsMelikyan GB, Lin S, Roth MG, Cohen FS
JournalMol Biol Cell
PubMed ID10359599
The amino acid sequence requirements of the transmembrane (TM) domain and cytoplasmic tail (CT) of the hemagglutinin (HA) of influenza virus in membrane fusion have been investigated. Fusion properties of wild-type HA were compared with those of chimeras consisting of the ectodomain of HA and the TM domain and/or CT ... More
Calcium binding protein 1 of the protozoan parasite Entamoeba histolytica interacts with actin and is involved in cytoskeleton dynamics.
AuthorsSahoo N, Labruyère E, Bhattacharya S, Sen P, Guillén N, Bhattacharya A
JournalJ Cell Sci
PubMed ID15252130
Blocking expression of EhCaBP1, a calmodulin-like, four EF-hand protein from the protozoan parasite Entamoeba histolytica, resulted in inhibition of cellular proliferation. In this paper we report that EhCaBP1 is involved in dynamic changes of the actin cytoskeleton. Both endocytosis and phagocytosis were severely impaired in cells where EhCaBP1 expression was ... More
Alkylglycerol opening of the blood-brain barrier to small and large fluorescence markers in normal and C6 glioma-bearing rats and isolated rat brain capillaries.
AuthorsErdlenbruch B, Alipour M, Fricker G, Miller DS, Kugler W, Eibl H, Lakomek M
JournalBr J Pharmacol
PubMed ID14597599
1. The blood-brain barrier (BBB) represents the major impediment to successful delivery of therapeutic agents to target tissue within the central nervous system. Intracarotid alkylglycerols have been shown to increase the transfer of chemotherapeutics across the BBB. 2. We investigated the spatial distribution of intracarotid fluorescein sodium and intravenous lissamine-rhodamine ... More
Use of inert gas jets to measure the forces required for mechanical gene transfection.
Authors
JournalBiomed Eng Online
PubMed ID22963645
Three-Dimensional Blood-Brain Barrier Model for in vitro Studies of Neurovascular Pathology.
Authors
JournalSci Rep
PubMed ID26503597
Role of TGF-β receptor III localization in polarity and breast cancer progression.
Authors
JournalMol Biol Cell
PubMed ID24870032
Islet vascularization is regulated by primary endothelial cilia via VEGF-A-dependent signaling.
Authors
JournalElife
PubMed ID33200981
Evaluation of β-Catenin Inhibition of Axitinib and Nitazoxanide in Human Monocyte-Derived Dendritic Cells.
Authors
JournalBiomedicines
PubMed ID34440153
Connexin43 promotes angiogenesis through activating the HIF-1α/VEGF signaling pathway under chronic cerebral hypoperfusion.
Authors
JournalJ Cereb Blood Flow Metab
PubMed ID33899559
Cancer Cells Resist Mechanical Destruction in Circulation via RhoA/Actomyosin-Dependent Mechano-Adaptation.