'Alveolar macrophages (AMs) play a major role in host defense against microbial infections in the lung. To perform this function, these cells must ingest and destroy pathogens, generally in phagosomes, as well as secrete a number of products that signal other immune cells to respond. Recently, we demonstrated that murine ... More
Individual acidic organelle pH measurements by capillary electrophoresis.
AuthorsChen Y, Arriaga EA
JournalAnal Chem
PubMed ID16448056
'This report describes the pH measurement of individual acidic organelles isolated from the human leukemia CCRF-CEM and CEM/C2 cells. These cells were allowed to endocytose fluorescein tetramethylrhodamine dextran (FRD), a ratiometric probe that has fluorescein as a pH-dependent fluorophore and tetramethylrhodamine as a pH-independent fluorophore. Isolated organelle fractions from these ... More
Second messengers regulate endosomal acidification in Swiss 3T3 fibroblasts.
AuthorsZen K, Biwersi J, Periasamy N, Verkman AS
JournalJ Cell Biol
PubMed ID1382079
Acidification of the endosomal pathway is important for ligand and receptor sorting, toxin activation, and protein degradation by lysosomal acid hydrolases. Fluorescent probes and imaging methods were developed to measure pH to better than 0.2 U accuracy in individual endocytic vesicles in Swiss 3T3 fibroblasts. Endosomes were pulse labeled with ... More
Transport from late endosomes to lysosomes, but not sorting of integral membrane proteins in endosomes, depends on the vacuolar proton pump.
Authorsvan Weert AW, Dunn KW, Gueze HJ, Maxfield FR, Stoorvogel W
JournalJ Cell Biol
PubMed ID7642700
Endocytosed proteins are sorted in early endosomes to be recycled to the plasma membrane or transported further into the degradative pathway. We studied the role of endosomes acidification on the endocytic trafficking of the transferrin receptor (TfR) as a representative for the recycling pathway, the cation-dependent mannose 6-phosphate receptor (MPR) ... More
Relocalized redox-active lysosomal iron is an important mediator of oxidative-stress-induced DNA damage.
AuthorsKurz T, Leake A, Von Zglinicki T, Brunk UT
JournalBiochem J
PubMed ID14670081
Oxidative damage to nuclear DNA is known to involve site-specific Fenton-type chemistry catalysed by redox-active iron or copper in the immediate vicinity of DNA. However, the presence of transition metals in the nucleus has not been shown convincingly. Recently, it was proposed that a major part of the cellular pool ... More
V-ATPase (Vacuolar ATPase) Activity Required for ABCA1 (ATP-Binding Cassette Protein A1)-Mediated Cholesterol Efflux.
Authors
JournalArterioscler Thromb Vasc Biol
PubMed ID30354238
Soluble adenylyl cyclase is essential for proper lysosomal acidification.
Authors
JournalJ Gen Physiol
PubMed ID27670898
Quantitative trait locus mapping identifies the Gpnmb gene as a modifier of mouse macrophage lysosome function.