In situ localization of paclitaxel binding structures: Labeling with a paclitaxel fluorescent analogue.
AuthorsBicamumpaka C, Page M
JournalInt J Mol Med
PubMed ID9855683
'Microtubules are a major component of cell cytoskeleton. Microtubules constitute the cellular target of paclitaxel. The interaction of paclitaxel with microtubules causes an increase in tubulin polymerization and microtubules stabilization, leading to a G2/M phase cell cycle arrest and cell death by apoptosis. Three paclitaxel fluorescent analogues were prepared by ... More
Peptide-based targeting of fluorophores to peroxisomes in living cells.
AuthorsPap EH, Dansen TB, Wirtz KW
JournalTrends Cell Biol
PubMed ID11146278
'Peptides carrying different fluorophores can be designed to incorporate spontaneously into living cells when added to the medium. By incorporating the peroxisome-targeting sequence PTS1, the peptide is recognized by the protein-import machinery of peroxisomes and, as a result, can accumulate in these organelles. Depending on the cell type, an inhibitor ... More
Patterns of intracellular compartmentalization, trafficking and acidification of 5'-fluorescein labeled phosphodiester and phosphorothioate oligodeoxynucleotides in HL60 cells.
AuthorsTonkinson JL, Stein CA
JournalNucleic Acids Res
PubMed ID7937155
'We have examined the intracellular compartmentalization and trafficking of fluorescein labeled (F) phosphodiester (PO) and phosphorothioate (PS) oligodeoxynucleotides (oligos) in HL60 cells. A series of F-oligos (PO and PS) were incubated for 6 hrs. with HL60 cells and the mean intracellular fluorescence determined by flow cytometry. The F signal was ... More
Using BODIPY dye-primer chemistry in large-scale sequencing.
AuthorsMuzny DM, Metzker ML, Bouck J, Gorrell JH, Ding Y, Maxim E, Gibbs RA
JournalIEEE Eng Med Biol Mag
PubMed ID9824768
Electrophoretically uniform fluorescent dyes for automated DNA sequencing.
AuthorsMetzker ML, Lu J, Gibbs RA
JournalScience
PubMed ID8596915
A class of dyes, BODIPY fluorophores, has been identified for automated DNA sequencing that has improved spectral characteristics compared with conventional fluorescein and rhodamine dyes. Single and double BODIPY dye primers were characterized in commercially available DNA sequencers and showed uniform electrophoretic mobilities and high fluorescence intensities. The improved physical ... More
Characterization of dye-induced mobility shifts affecting DNA sequencing in poly(ethylene oxide) sieving matrix.
AuthorsTan H, Yeung ES
JournalElectrophoresis
PubMed ID9504827
The influence of three classes of fluorescence labels including dipyrrometheneboron difluoride (BODIPY), energy transfer (ET) and conventional fluorescein and rhodamine (ABI), on DNA sequencing has been examined with laser-induced fluorescence detection and poly(ethylene oxide)-filled capillary electrophoresis. DNA sequencing fragments were generated by dye-labeled primer cycle-sequencing reactions in a hot-air thermal ... More
Comparison of fluorescence energy transfer primers with different donor-acceptor dye combinations.
AuthorsHung SC, Mathies RA, Glazer AN
JournalAnal Biochem
PubMed ID9448839
Fluorescence energy transfer (ET) primers are far superior to single dye-labeled primers as labels for DNA sequencing and polymerase chain reaction amplification. We compare here ET primers with different donor and acceptor dye combinations with respect to the relative acceptor fluorescence emission intensity and the amount of residual donor fluorescence ... More
The recruitment of Raf-1 to membranes is mediated by direct interaction with phosphatidic acid and is independent of association with Ras.
AuthorsRizzo MA, Shome K, Watkins SC, Romero G
JournalJ Biol Chem
PubMed ID10801816
The serine/threonine kinase Raf-1 is an essential component of the MAPK cascade. Activation of Raf-1 by extracellular signals is initiated by association with intracellular membranes. Recruitment of Raf-1 to membranes has been reported to be mediated by direct association with Ras and by the phospholipase D product phosphatidic acid (PA). ... More
Quantitation of mixed-base populations of HIV-1 variants by automated DNA sequencing with BODIPY dye-labeled primers.
AuthorsMetzker ML, Ansari-Lari MA, Liu XM, Holder DJ, Gibbs RA
JournalBiotechniques
PubMed ID9762443
Direct DNA sequencing of human immunodeficiency virus type 1 (HIV-1) pol and env gene regions was characterized for accuracy and precision. Restricted maximum likelihood (REML) analysis of molecular clone reconstruction experiments using a primer-walking strategy showed that the BODIPY and BODIPY energy-transfer (BET) dye primers sets were significantly more accurate ... More
Peptide-based targeting of fluorophores to organelles in living cells.
AuthorsPap EH, Dansen TB, van Summeren R, Wirtz KW
JournalExp Cell Res
PubMed ID11302694
Peptides carrying organelle-specific import or retention sequences can target the fluorophore BODIPY(581/591) to the nucleus, peroxisomes, endoplasmic reticulum (ER), or the trans-Golgi network (TGN). The peroxisomal peptide contains the PTS1 sequence AKL. For targeting to the ER or TGN, the peptides carry the retention sequences KDEL and SDYQRL, respectively. A ... More
Direct, ultrasensitive, and selective optical detection of protein toxins using multivalent interactions.
AuthorsSong X, Swanson BI
JournalAnal Chem
PubMed ID10366891
Three highly sensitive, selective, and reagent-free optical signal transduction methods for detection of polyvalent proteins have been developed by directly coupling distance-dependent fluorescence self-quenching and/or resonant-energy transfer to the protein-receptor binding events. The ganglioside GM1, as the recognition unit for cholera toxin (CT), was covalently labeled with fluorophores and then ... More
Targeted fluorescent probes in peroxisome function.
AuthorsDansen TB, Pap EHW Wanders RJ, Wirtz KW
JournalHistochem J
PubMed ID11432641
Fluorescent peptides form a new generation of analytical tools for visualizing intracellular processes and molecular interactions at the level of single cells. The peptide-based reporters combine the sensitivity of fluorescence detection with the information specificity of amino acid sequences. Recently we have succeeded in targeting a fluorescent heptapeptide (acetyl-CKGGAKL) carrying ... More