JC-9 Dye (Mitochondrial Membrane Potential Probe)

Zitierungen und Referenzen (5)

Invitrogen™

JC-9 Dye (Mitochondrial Membrane Potential Probe)

Die ratiometrische mitochondriale Membranpotentialsonde JC-9 weist eine grüne Fluoreszenz auf, die unabhängig vom Membranpotential ist und eine Zunahme der rotenWeitere Informationen

Katalognummer	Menge
D22421	5 mg

Katalognummer D22421

Preis (EUR)

924,00

Menge:

Preis (EUR)

924,00

Die ratiometrische mitochondriale Membranpotentialsonde JC-9 weist eine grüne Fluoreszenz auf, die unabhängig vom Membranpotential ist und eine Zunahme der roten Fluoreszenz bei hyperpolarisierten Membranpotentialen aufweist.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

NachweisverfahrenFluoreszent

Menge5 mg

VersandbedingungRaumtemperatur

Subzelluläre LokalisationMitochondrien

FarbeGrün

Zur Verwendung mit (Geräte)Fluoreszenzmikroskop, Durchflusszytometer

ProdukttypMitochondrienmembranpotential-Sonde

Unit SizeEach

Inhalt und Lagerung

Lagerung bei Raumtemperatur und vor Licht geschützt

Häufig gestellte Fragen (FAQ)

I am seeing high background outside of my neuronal cells when using membrane potential indicators. What can I do to reduce background?

If you use our FluoVolt Membrane Potential Kit (Cat. No. F10488), the kit provides a background suppressor to reduce this problem. For other indicators, consider the use of BackDrop Background Suppressor (Cat no. R37603, B10511, and B10512).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between fast and slow-response membrane potential probes?

Molecules that change their structure in response to the surrounding electric field can function as fast-response probes for the detection of transient (millisecond) potential changes. Slow-response dyes function by entering depolarized cells and binding to proteins or membranes. Increased depolarization results in additional dye influx and an increase in fluorescence, while hyperpolarization is indicated by a decrease in fluorescence. Fast-response probes are commonly used to image electrical activity from intact heart tissues or measure membrane potential changes in response to pharmacological stimuli. Slow-responding probes are often used to explore mitochondrial function and cell viability.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What type of membrane potential indicators do you offer and how should I choose one for my experiment?

A membrane potential indicator selection guide can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Zitierungen und Referenzen (5)

Zitierungen und Referenzen

Abstract

Synthesis and identification of small molecules that potently induce apoptosis in melanoma cells through G1 cell cycle arrest.

Authors:Dothager RS, Putt KS, Allen BJ, Leslie BJ, Nesterenko V, Hergenrother PJ,

Journal:J Am Chem Soc

PubMed ID:15954774

'Late-stage malignant melanoma is a cancer that is refractory to current chemotherapeutic treatments. The average survival time for patients with such a diagnosis is 6 months. In general, the vast majority of anticancer drugs operate through induction of cell cycle arrest and cell death in either the DNA synthesis (S) ... More

Chlorophenols and chlorocatechols induce apoptosis in human lymphocytes (in vitro).

Authors:Michalowicz J, Sicinska P,

Journal:Toxicol Lett

PubMed ID:19766705

In this work the effect of 2,4,5-trichlorophenol (2,4,5-TCP), pentachlorophenol (PCP), 4,6-dichloroguaiacol (4,6-DCG), tetrachloroguaiacol (TeCG), 4,5-dichlorocatechol (4,5-DCC) and tetrachlorocatechol (TeCC) on the induction of apoptosis in human peripheral blood lymphocytes was examined. The analysis of the changes in mitochondrial transmembrane potential (DeltaPsi(m)) was performed using JC-9 fluorescent probe. It was noted ... More

Markers of apoptosis: methods for elucidating the mechanism of apoptotic cell death from the nervous system.

Authors:Smyth PG, Berman SA,

Journal:Biotechniques

PubMed ID:11911667

Apoptosis is a highly conserved energy-requiring program for non-inflammatory cell death that is important in both normal physiology and disease. Numerous techniques have been used to study apoptosis. In the nervous system, apoptosis is necessary for normal development, but it also occurs in many acute and chronic pathologic conditions. This ... More

DGAT1-Dependent Lipid Droplet Biogenesis Protects Mitochondrial Function during Starvation-Induced Autophagy.

Authors:

Journal:Dev Cell

PubMed ID:28697336

A role for mitochondria in the establishment and maintenance of the maize root quiescent center.

Authors:

Journal:Plant Physiol

PubMed ID:16443698