DNP-X, SE, 6-(2,4-Dinitrophenyl)aminohexanoic Acid, Succinimidyl Ester

Citations & References (5)

Invitrogen™

DNP-X, SE, 6-(2,4-Dinitrophenyl)aminohexanoic Acid, Succinimidyl Ester

Catalog Number	Quantity
D2248 also known as D-2248	25 mg

Catalog number D2248

also known as D-2248

Price (USD)

315.00

Quantity:

25 mg

Price (USD)

315.00

The amine-reactive DNP-X succinimidyl ester can be used bioconjugates that can be detected with anti-dinitrophenyl (anti-DNP) antibodies. This reactive hapten contains an additional seven-atom aminohexanoyl spacer ('X') between the fluorophore and the succinimidyl ester group. This spacer helps to separate increase accessibility to anti-DNP antibodies.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Chemical ReactivityAmine

Label or DyeDNP

Product TypeDNP-X Succinimidyl Ester

Quantity25 mg

Reactive MoietyActive Ester, Succinimidyl Ester

Shipping ConditionRoom Temperature

Label TypeBiotin and Other Haptens

Unit SizeEach

Contents & Storage

Store in freezer (-5 to -30°C) and protect from light.

Citations & References (5)

Citations & References

Abstract

Endosomal density shift is related to a decrease in fusion capacity.

Authors:Diaz R, Colombo MI, Koval M, Mayorga L, Stahl P

Journal:Eur J Cell Biol

PubMed ID:1802709

'Dinitrophenol (DNP)-beta-glucuronidase and mannosylated anti-DNP IgG, which are endocytosed by the mannose receptor and delivered to lysosomes, were previously developed as probes for examination of fusion between early endosomes in a cell-free system. In this study, these probes were found to be transported by intact cells to endocytic vesicles with ... More

Tyramide signal amplification for analysis of kinase activity by intracellular flow cytometry.

Authors:Clutter MR, Heffner GC, Krutzik PO, Sachen KL, Nolan GP,

Journal:Cytometry A

PubMed ID:20824632

'Intracellular flow cytometry permits quantitation of diverse molecular targets at the single-cell level. However, limitations in detection sensitivity inherently restrict the method, sometimes resulting in the inability to measure proteins of very low abundance or to differentiate cells expressing subtly different protein concentrations. To improve these measurements, an enzymatic amplification ... More

Impact of hapten presentation on antibody binding at lipid membrane interfaces.

Authors:Jung H, Yang T, Lasagna MD, Shi J, Reinhart GD, Cremer PS,

Journal:Biophys J

PubMed ID:18199665

We report the effects of ligand presentation on the binding of aqueous proteins to solid supported lipid bilayers. Specifically, we show that the equilibrium dissociation constant can be strongly affected by ligand lipophilicity and linker length/structure. The apparent equilibrium dissociation constants (K(D)) were compared for two model systems, biotin/anti-biotin and ... More

IgE-mediated activation of NK cells through Fc gamma RIII.

Authors:Arase N, Arase H, Hirano S, Yokosuka T, Sakurai D, Saito T

Journal:J Immunol

PubMed ID:12626560

NK cells express Fc gamma RIII (CD16), which is responsible for IgG-dependent cell cytotoxicity and for production of several cytokines and chemokines. Whereas Fc gamma RIII on NK cells is composed of both Fc gamma RIII alpha and FcR gamma chains, that on mast cells is distinct from NK cells ... More

Synthetic mimics of small Mammalian cell surface receptors.

Authors:Boonyarattanakalin S, Martin SE, Dykstra SA, Peterson BR

Journal:J Am Chem Soc

PubMed ID:15600339

Receptors on the surface of mammalian cells promote the uptake of cell-impermeable ligands by receptor-mediated endocytosis. To mimic this process, we synthesized small molecules designed to project anti-dinitrophenyl antibody-binding motifs from the surface of living Jurkat lymphocytes. These synthetic receptors comprise N-alkyl derivatives of 3beta-cholesterylamine as the plasma membrane anchor ... More