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Citas y referencias (5)
Invitrogen™
DNP-X, SE, 6-(2,4-Dinitrophenyl)aminohexanoic Acid, Succinimidyl Ester
El éster de succinimidilo DNP-X, reactivo a la amina, puede utilizarse con bioconjugados que pueden detectarse con anticuerpos antidinitrofenilo (anti-DNP).Más información
| Número de catálogo | Cantidad |
|---|---|
| D2248 también denominado D-2248 | 25 mg |
Número de catálogo D2248
también denominado D-2248
Precio (MXN)
-
Cantidad:
25 mg
El éster de succinimidilo DNP-X, reactivo a la amina, puede utilizarse con bioconjugados que pueden detectarse con anticuerpos antidinitrofenilo (anti-DNP). Este hapteno reactivo contiene un espaciador de aminohexanoil de siete átomos («X») adicional entre el fluoróforo y el grupo de éster de succinimidil. Este espaciador ayuda a separar y aumenta la accesibilidad a los anticuerpos antiDNP.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Reactividad químicaAmina
Etiqueta o tinteDNP
Tipo de productoÉster de succinimidilo DNP-X
Cantidad25 mg
Fracción reactivaEster activo, succinimidilo éster
Condiciones de envíoTemperatura ambiente
Tipo de etiquetaBiotina y otros haptenos
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador (de – 5 a – 30 °C) y proteger de la luz.
Citations & References (5)
Citations & References
Abstract
Endosomal density shift is related to a decrease in fusion capacity.
Journal:Eur J Cell Biol
PubMed ID:1802709
'Dinitrophenol (DNP)-beta-glucuronidase and mannosylated anti-DNP IgG, which are endocytosed by the mannose receptor and delivered to lysosomes, were previously developed as probes for examination of fusion between early endosomes in a cell-free system. In this study, these probes were found to be transported by intact cells to endocytic vesicles with
Tyramide signal amplification for analysis of kinase activity by intracellular flow cytometry.
Journal:Cytometry A
PubMed ID:20824632
'Intracellular flow cytometry permits quantitation of diverse molecular targets at the single-cell level. However, limitations in detection sensitivity inherently restrict the method, sometimes resulting in the inability to measure proteins of very low abundance or to differentiate cells expressing subtly different protein concentrations. To improve these measurements, an enzymatic amplification
Impact of hapten presentation on antibody binding at lipid membrane interfaces.
Journal:Biophys J
PubMed ID:18199665
We report the effects of ligand presentation on the binding of aqueous proteins to solid supported lipid bilayers. Specifically, we show that the equilibrium dissociation constant can be strongly affected by ligand lipophilicity and linker length/structure. The apparent equilibrium dissociation constants (K(D)) were compared for two model systems, biotin/anti-biotin and
IgE-mediated activation of NK cells through Fc gamma RIII.
Journal:J Immunol
PubMed ID:12626560
NK cells express Fc gamma RIII (CD16), which is responsible for IgG-dependent cell cytotoxicity and for production of several cytokines and chemokines. Whereas Fc gamma RIII on NK cells is composed of both Fc gamma RIII alpha and FcR gamma chains, that on mast cells is distinct from NK cells
Synthetic mimics of small Mammalian cell surface receptors.
Journal:J Am Chem Soc
PubMed ID:15600339
Receptors on the surface of mammalian cells promote the uptake of cell-impermeable ligands by receptor-mediated endocytosis. To mimic this process, we synthesized small molecules designed to project anti-dinitrophenyl antibody-binding motifs from the surface of living Jurkat lymphocytes. These synthetic receptors comprise N-alkyl derivatives of 3beta-cholesterylamine as the plasma membrane anchor