DNase X is a glycosylphosphatidylinositol-anchored membrane enzyme that provides a barrier to endocytosis-mediated transfer of a foreign gene.
AuthorsShiokawa D, Matsushita T, Shika Y, Shimizu M, Maeda M, Tanuma S
JournalJ Biol Chem
PubMed ID17416904
'DNase X is the first mammalian DNase to be isolated that is homologous to DNase I. In this study, we have examined its function using a novel monoclonal antibody and showed it to be expressed on the cell surface as a glycosylphosphatidylinositolanchored membrane protein. High level expression was observed in ... More
Impaired trafficking of connexins in androgen-independent human prostate cancer cell lines and its mitigation by alpha-catenin.
AuthorsGovindarajan R, Zhao S, Song XH, Guo RJ, Wheelock M, Johnson KR, Mehta PP
JournalJ Biol Chem
PubMed ID12205082
'Gap junctions, composed of connexins, provide a pathway of direct intercellular communication for the diffusion of small molecules between cells. Evidence suggests that connexins act as tumor suppressors. We showed previously that expression of connexin-43 and connexin-32 in an indolent prostate cancer cell line, LNCaP, resulted in gap junction formation ... More
Local calcium transients regulate the spontaneous motility of dendritic filopodia.
AuthorsLohmann C, Finski A, Bonhoeffer T
JournalNat Neurosci
PubMed ID15711541
'During development, dendrites, and in particular dendritic filopodia, undergo extensive structural remodeling, presumably to help establish synaptic contacts. Here, we investigated the role of calcium signaling in dendritic plasticity by simultaneously recording calcium dynamics and filopodial growth in rat hippocampal slice cultures. Local calcium transients occurred in dendritic filopodia and ... More
Homotypic fusion of early endosomes: SNAREs do not determine fusion specificity.
AuthorsBrandhorst D, Zwilling D, Rizzoli SO, Lippert U, Lang T, Jahn R
JournalProc Natl Acad Sci U S A
PubMed ID16469845
'Membrane fusion in the secretory pathway is mediated by soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) proteins. Different fusion steps are thought to be effected by independent sets of SNAREs, but it is unclear whether specificity is determined by an intrinsic specificity of SNARE pairing or by upstream factors. Using a ... More
Evidence for early endosome-like fusion of recently endocytosed synaptic vesicles.
AuthorsRizzoli SO, Bethani I, Zwilling D, Wenzel D, Siddiqui TJ, Brandhorst D, Jahn R
JournalTraffic
PubMed ID17004320
'Early endosomes are well-established acceptor compartments of endocytic vesicles in many cell types. Little evidence of their existence or function has been obtained in synapses, and it is generally believed that synaptic vesicles recycle without passing through an endosomal intermediate. We show here that the early endosomal SNARE proteins are ... More
Persistent activity in a cortical-to-subcortical circuit: bridging the temporal gap in trace eyelid conditioning.
'We have addressed the source and nature of the persistent neural activity that bridges the stimulus-free gap between the conditioned stimulus (CS) and unconditioned stimulus (US) during trace eyelid conditioning. Previous work has demonstrated that this persistent activity is necessary for trace eyelid conditioning: CS-elicited activity in mossy fiber inputs ... More
Physiology of afferent neurons in larval zebrafish provides a functional framework for lateral line somatotopy.
AuthorsLiao JC, Haehnel M,
JournalJ Neurophysiol
PubMed ID22338025
'Fishes rely on the neuromasts of their lateral line system to detect water flow during behaviors such as predator avoidance and prey localization. Although the pattern of neuromast development has been a topic of detailed research, we still do not understand the functional consequences of its organization. Previous work has ... More
Effects of the noradrenergic system in rat white matter exposed to oxygen-glucose deprivation in vitro.
AuthorsNikolaeva MA, Richard S, Mouihate A, Stys PK,
JournalJ Neurosci
PubMed ID19211886
Norepinephrine (NE) is released in excess into the extracellular space during oxygen-glucose deprivation (OGD) in brain, increasing neuronal metabolism and aggravating glutamate excitoxicity. We used isolated rat optic nerve and spinal cord dorsal columns to determine whether the noradrenergic system influences axonal damage in white matter. Tissue was studied electrophysiologically ... More
Enterocyte TLR4 mediates phagocytosis and translocation of bacteria across the intestinal barrier.
AuthorsNeal MD, Leaphart C, Levy R, Prince J, Billiar TR, Watkins S, Li J, Cetin S, Ford H, Schreiber A, Hackam DJ,
JournalJ Immunol
PubMed ID16493066
Translocation of bacteria across the intestinal barrier is important in the pathogenesis of systemic sepsis, although the mechanisms by which bacterial translocation occurs remain largely unknown. We hypothesized that bacterial translocation across the intact barrier occurs after internalization of the bacteria by enterocytes in a process resembling phagocytosis and that ... More
Glycolipid trafficking in Drosophila undergoes pathway switching in response to aberrant cholesterol levels.
AuthorsHortsch R, Lee E, Erathodiyil N, Hebbar S, Steinert S, Lee JY, Chua DS, Kraut R,
JournalMol Biol Cell
PubMed ID20053687
In lipid storage diseases, the intracellular trafficking of sphingolipids is altered by conditions of aberrant cholesterol accumulation. Drosophila has been used recently to model lipid storage diseases, but the effects of sterol accumulation on sphingolipid trafficking are not known in the fly, and the trafficking of sphingolipids in general has ... More
Endosomal recycling controls plasma membrane area during mitosis.
AuthorsBoucrot E, Kirchhausen T
JournalProc Natl Acad Sci U S A
PubMed ID17483462
The shape and total surface of a cell and its daughters change during mitosis. Many cells round up during prophase and metaphase and reacquire their extended and flattened shape during cytokinesis. How does the total area of plasma membrane change to accommodate these morphological changes and by what mechanism is ... More
Endocytosis resumes during late mitosis and is required for cytokinesis.
AuthorsSchweitzer JK, Burke EE, Goodson HV, D'Souza-Schorey C
JournalJ Biol Chem
PubMed ID16207714
Recent work has underscored the importance of membrane trafficking events during cytokinesis. For example, targeted membrane secretion occurs at the cleavage furrow in animal cells, and proteins that regulate endocytosis also influence the process of cytokinesis. Nonetheless, the prevailing dogma is that endosomal membrane trafficking ceases during mitosis and resumes ... More
The role of LIP5 and CHMP5 in multivesicular body formation and HIV-1 budding in mammalian cells.
AuthorsWard DM, Vaughn MB, Shiflett SL, White PL, Pollock AL, Hill J, Schnegelberger R, Sundquist WI, Kaplan J
JournalJ Biol Chem
PubMed ID15644320
We examined the function of LIP5 in mammalian cells, because the yeast homologue Vta1p was recently identified as a protein required for multivesicular body (MVB) formation. LIP5 is predominantly a cytosolic protein. Depletion of LIP5 by small inhibitory RNA (siRNA) does not affect the distribution or morphology of early endosomes, ... More
Optimal transfection with the HK polymer depends on its degree of branching and the pH of endocytic vesicles.
AuthorsChen QR, Zhang L, Luther PW, Mixson AJ
JournalNucleic Acids Res
PubMed ID11884631
We have recently reported that liposomes in combination with histidine (HK)-containing polymers enhanced the expression of luciferase in transfected cells. In transformed or malignant cell lines, branched HK polymers (combined with liposome carriers) were significantly more effective than the linear HK polymer in stimulating gene expression. In the current study, ... More
Serotonin Induces Structural Plasticity of Both Extrinsic Modulating and Intrinsic Mediating Circuits In Vitro in Aplysia Californica.
AuthorsUpreti C, Konstantinov E, Kassabov SR, Bailey CH, Kandel ER
JournalCell Rep
PubMed ID31509754
'Long-term sensitization of the gill withdrawal reflex in Aplysia requires heterosynaptic, modulatory input that is mediated in part by the growth of new synaptic connections between sensory neurons and their follower cells (intrinsic mediating circuit). Whether modulatory interneurons (the extrinsic modulatory circuit) also display learning-related structural synaptic plasticity remains unknown. ... More
Arf6, JIP3, and dynein shape and mediate macropinocytosis.
AuthorsWilliamson CD, Donaldson JG
JournalMol Biol Cell
PubMed ID30969891
'Macropinocytosis is an actin-driven form of clathrin-independent endocytosis that generates an enlarged structure, the macropinosome. Although many studies focus on signaling molecules and phosphoinositides involved in initiating macropinocytosis, the commitment to forming a macropinosome and the handling of that membrane have not been studied in detail. Here we show in ... More
Accumulation of undegraded autophagosomes by expression of dominant-negative STX17 (syntaxin 17) mutants.
Authors
JournalAutophagy
PubMed ID28598244
A cerebellum-like circuit in the auditory system cancels responses to self-generated sounds.
Authors
JournalNat Neurosci
PubMed ID28530663
ER membranes exhibit phase behavior at sites of organelle contact.
AuthorsKing C, Sengupta P, Seo AY, Lippincott-Schwartz J
JournalProc Natl Acad Sci U S A
PubMed ID32179693
'The endoplasmic reticulum (ER) is the site of synthesis of secretory and membrane proteins and contacts every organelle of the cell, exchanging lipids and metabolites in a highly regulated manner. How the ER spatially segregates its numerous and diverse functions, including positioning nanoscopic contact sites with other organelles, is unclear. ... More
Ventral hippocampal projections to the medial prefrontal cortex regulate social memory.
Authors
JournalElife
PubMed ID31112129
The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.
Authors
JournalCell Mol Life Sci
PubMed ID33340069
Specific populations of basal ganglia output neurons target distinct brain stem areas while collateralizing throughout the diencephalon.
Authors
JournalNeuron
PubMed ID33823137
Intracellular Calcium Mobilization Is Required for Sonic Hedgehog Signaling.
Authors
JournalDev Cell
PubMed ID29754802
Genetic impairment of parasite myosin motors uncovers the contribution of host cell membrane dynamics to Toxoplasma invasion forces.
Authors
JournalBMC Biol
PubMed ID27829452
Sensitive detection of lysosomal membrane permeabilization by lysosomal galectin puncta assay.
Authors
JournalAutophagy
PubMed ID26114578
Loss of Christianson Syndrome Na+/H+ Exchanger 6 (NHE6) Causes Abnormal Endosome Maturation and Trafficking Underlying Lysosome Dysfunction in Neurons.
Authors
JournalJ Neurosci
PubMed ID34526390
Inhibiting store-operated calcium entry attenuates white matter secondary degeneration following SCI.
AuthorsOrem BC, Partain SB, Stirling DP
JournalNeurobiol Dis
PubMed ID31846736
Axonal degeneration plays a key role in the pathogenesis of numerous neurological disorders including spinal cord injury. After the irreversible destruction of the white matter elements during the primary (mechanical) injury, spared axons and their supporting glial cells begin to breakdown causing an expansion of the lesion site. Here we ... More
Dense projection of Stilling's nucleus spinocerebellar axons that convey tail proprioception to the midline area in lobule VIII of the mouse cerebellum.
AuthorsLuo Y, Onozato T, Wu X, Sasamura K, Sakimura K, Sugihara I
JournalBrain Struct Funct
PubMed ID31955293
The cerebellar cortex has dual somatotopic representation, broadly in the anterior lobules and narrowly in the posterior lobules. However, the somatotopy has not been well understood in vermal lobule VIII, located in the center of the posterior representation. Here, we examined the axonal projections and somatosensory representation of the midline ... More