A role for phosphoinositide 3-kinase in the completion of macropinocytosis and phagocytosis by macrophages.
AuthorsAraki N, Johnson MT, Swanson JA
JournalJ Cell Biol
PubMed ID8947549
'Phosphoinositide 3-kinase (PI 3-kinase) has been implicated in growth factor signal transduction and vesicular membrane traffic. It is thought to mediate the earliest steps leading from ligation of cell surface receptors to increased cell surface ruffling. We show here that inhibitors of PI 3-kinase inhibit endocytosis in macrophages, not by ... More
Permeability of single capillaries to intermediate-sized colored solutes.
AuthorsCurry FE, Huxley VH, Adamson RH
JournalAm J Physiol
PubMed ID6604463
'The fiber matrix theory of capillary permeability was evaluated by measuring permeability coefficients to colored solutes with Stokes radii between 0.5 and 1.76 nm. In vivo calibration of a microscope photometer established the range of linearity for optical density measurements from a rectangular window that included the test capillary and ... More
Single-cell electroporation for gene transfer in vivo.
AuthorsHaas K, Sin WC, Javaherian A, Li Z, Cline HT
JournalNeuron
PubMed ID11301019
We report an electroporation technique for targeting gene transfer to individual cells in intact tissue. Electrical stimulation through a micropipette filled with DNA or other macromolecules electroporates a single cell at the tip of the micropipette. Electroporation of a plasmid encoding enhanced green fluorescent protein (GFP) into the brain of ... More
Neural organization and visual processing in the anterior optic tubercle of the honeybee brain.
AuthorsMota T, Yamagata N, Giurfa M, Gronenberg W, Sandoz JC,
JournalJ Neurosci
PubMed ID21832175
The honeybee Apis mellifera represents a valuable model for studying the neural segregation and integration of visual information. Vision in honeybees has been extensively studied at the behavioral level and, to a lesser degree, at the physiological level using intracellular electrophysiological recordings of single neurons. However, our knowledge of visual ... More
Assessing the sensitivity of commercially available fluorophores to the intracellular environment.
AuthorsChen AK, Cheng Z, Behlke MA, Tsourkas A,
JournalAnal Chem
PubMed ID18700780
The use of fluorescence has become commonplace in the biological sciences, with many studies utilizing probes based on commercially available fluorophores to provide insight into cell function and behavior. As these imaging applications become more advanced, it becomes increasingly important to acquire accurate quantitative measurements of the fluorescence signal. Absolute ... More
Mammalian reovirus, a nonfusogenic nonenveloped virus, forms size-selective pores in a model membrane.
AuthorsAgosto MA, Ivanovic T, Nibert ML
JournalProc Natl Acad Sci U S A
PubMed ID17053074
During cell entry, reovirus particles with a diameter of 70-80 nm must penetrate the cellular membrane to access the cytoplasm. The mechanism of penetration, without benefit of membrane fusion, is not well characterized for any such nonenveloped animal virus. Lysis of RBCs is an in vitro assay for the membrane ... More
A novel method for simultaneous anterograde and retrograde labeling of spinal cord motor tracts in the same animal.
AuthorsTsai EC, van Bendegem RL, Hwang SW, Tator CH
JournalJ Histochem Cytochem
PubMed ID11511680
Examination of repaired spinal cord tracts has usually required separate groups of animals for anterograde and retrograde tracing owing to the incompatibility of techniques such as tissue fixation. However, anterograde and retrograde labeling of different animals subjected to the same repair may not allow accurate examination of that repair strategy ... More
Properties and regulation of gap junctional hemichannels in the plasma membranes of cultured cells.
AuthorsLi H, Liu TF, Lazrak A, Peracchia C, Goldberg GS, Lampe PD, Johnson RG
JournalJ Cell Biol
PubMed ID8769424
During the assembly of gap junctions, a hemichannel in the plasma membrane of one cell is thought to align and dock with another in an apposed membrane to form a cell-to-cell channel. We report here on the existence and properties of nonjunctional, plasma membrane connexin43 (Cx43) hemichannels. The opening of ... More
Exocytosis and endocytosis of small vesicles in PC12 cells studied with TEPIQ (two-photon extracellular polar-tracer imaging-based quantification) analysis.
AuthorsLiu TT, Kishimoto T, Hatakeyama H, Nemoto T, Takahashi N, Kasai H
JournalJ Physiol
PubMed ID16150796
We investigated exocytosis of PC12 cells using two-photon excitation imaging and extracellular polar tracers (TEP imaging) in the lateral membranes not facing the glass-cover slip. Upon photolysis of a caged Ca2+ compound, TEP imaging with FM1-43 (a polar membrane tracer) detected massive exocytosis of vesicles with a time constant of ... More
Alveolar macrophage-derived extracellular vesicles inhibit endosomal fusion of influenza virus.
Authors
JournalEMBO J
PubMed ID32643835
The Effector Domain Region of the Vibrio vulnificus MARTX Toxin Confers Biphasic Epithelial Barrier Disruption and Is Essential for Systemic Spread from the Intestine.
Authors
JournalPLoS Pathog
PubMed ID28060924
TNF Receptor 1 Promotes Early-Life Immunity and Protects against Colitis in Mice.
Authors
JournalCell Rep
PubMed ID33086075
Single-particle imaging of stress-promoters induction reveals the interplay between MAPK signaling, chromatin and transcription factors.
Authors
JournalNat Commun
PubMed ID32576833
Membrane binding by tBid initiates an ordered series of events culminating in membrane permeabilization by Bax.
Authors
JournalCell
PubMed ID19062087
Human norovirus culture in B cells.
Authors
JournalNat Protoc
PubMed ID26513671
Hemozoin Promotes Lung Inflammation via Host Epithelial Activation.
Authors
JournalMBio
PubMed ID33563839
A switch in pdgfrb+ cell-derived ECM composition prevents inhibitory scarring and promotes axon regeneration in the zebrafish spinal cord.
Authors
JournalDev Cell
PubMed ID33412105
Astrocytic neogenin/netrin-1 pathway promotes blood vessel homeostasis and function in mouse cortex.
Authors
JournalJ Clin Invest
PubMed ID32853179
Cancer Cells Resist Mechanical Destruction in Circulation via RhoA/Actomyosin-Dependent Mechano-Adaptation.