Novel naphthylstyryl-pyridium potentiometric dyes offer advantages for neural network analysis.
AuthorsObaid AL, Loew LM, Wuskell JP, Salzberg BM
JournalJ Neurosci Methods
PubMed ID15003384
'The submucous plexus of the guinea pig intestine is a quasi-two-dimensional mammalian neural network that is particularly amenable to study using multiple site optical recording of transmembrane voltage (MSORTV) [Biol. Bull. 183 (1992) 344; J. Neurosci. 19 (1999) 3073]. For several years the potentiometric dye of choice for monitoring the ... More
Cholesterol-enriched lipid domains can be visualized by di-4-ANEPPDHQ with linear and nonlinear optics.
AuthorsJin L, Millard AC, Wuskell JP, Clark HA, Loew LM
JournalBiophys J
PubMed ID15879475
We present a membrane-staining dye, di-4-ANEPPDHQ, which differentiates liquid-ordered phases from liquid-disordered phases coexisting in model membranes under both linear and nonlinear microscopies. The dye's fluorescence emission spectrum is blue-shifted 60 nm in liquid-ordered phases compared with liquid-disordered phases, and shows strong second harmonic generation in the liquid-disordered phase compared ... More
Spectral characterization of the voltage-sensitive dye di-4-ANEPPDHQ applied to probing live primary and immortalized neurons.
AuthorsWang Y, Jing G, Perry S, Bartoli F, Tatic-Lucic S,
JournalOpt Express
PubMed ID19158915
Spectral properties of a recently developed voltage-sensitive dye, di-4-ANEPPDHQ, were characterized as the dye was dissolved in the solvent dimethyl sulfoxide as the stock solution, in Hank's buffered salt solution as the staining solution, and bound to the plasma membrane of primary rat hippocampal neurons and immortalized mouse hypothalamic neurons ... More
Laurdan and di-4-ANEPPDHQ do not respond to membrane-inserted peptides and are good probes for lipid packing.
AuthorsDinic J, Biverståhl H, Mäler L, Parmryd I,
JournalBiochim Biophys Acta
PubMed ID20937246
Laurdan and di-4-ANEPPDHQ are used as probes for membrane order, with a blue shift in emission for membranes in liquid-ordered (lo) phase relative to membranes in liquid-disordered (ld) phase. Their use as membrane order probes requires that their spectral shifts are unaffected by membrane proteins, which we have examined by ... More
Characterization and application of a new optical probe for membrane lipid domains.
AuthorsJin L, Millard AC, Wuskell JP, Dong X, Wu D, Clark HA, Loew LM
JournalBiophys J
PubMed ID16415047
In this article, we characterize the fluorescence of an environmentally sensitive probe for lipid membranes, di-4-ANEPPDHQ. In large unilamellar lipid vesicles (LUVs), its emission spectrum shifts up to 30 nm to the blue with increasing cholesterol concentration. Independently, it displays a comparable blue shift in liquid-ordered relative to liquid-disordered phases. ... More
Membrane therapy using DHA suppresses epidermal growth factor receptor signaling by disrupting nanocluster formation.
Authors
JournalJ Lipid Res
PubMed ID33515553
Mechanism of Cell Penetration by Permeabilization of Late Endosomes: Interplay between a Multivalent TAT Peptide and Bis(monoacylglycero)phosphate.
Authors
JournalCell Chem Biol
PubMed ID32783962
High lipid order of Arabidopsis cell-plate membranes mediated by sterol and DYNAMIN-RELATED PROTEIN1A function.
Authors
JournalPlant J
PubMed ID25234576
Drought stress stimulates endocytosis and modifies membrane lipid order of rhizodermal cells of Medicago truncatula in a genotype-dependent manner.
Authors
JournalBMC Plant Biol
PubMed ID31138155
Chemotherapy induced PRL3 expression promotes cancer growth via plasma membrane remodeling and specific alterations of caveolae-associated signaling.
Authors
JournalCell Commun Signal
PubMed ID30157875
AdipoRon Attenuates Wnt Signaling by Reducing Cholesterol-Dependent Plasma Membrane Rigidity.
Authors
JournalBiophys J
PubMed ID31630812
Effects of titanium dioxide and zinc oxide nano-materials on lipid order in model membranes.
Authors
JournalBiochim Biophys Acta Biomembr
PubMed ID32304756
Delineating the Rules for Structural Adaptation of Membrane-Associated Proteins to Evolutionary Changes in Membrane Lipidome.
Authors
JournalCurr Biol
PubMed ID31956022
4D analysis of malaria parasite invasion offers insights into erythrocyte membrane remodeling and parasitophorous vacuole formation.
Authors
JournalNat Commun
PubMed ID34131147
SIRT1 regulates sphingolipid metabolism and neural differentiation of mouse embryonic stem cells through c-Myc-SMPDL3B.