High spatial resolution confocal microscopy of young MDCK cells stained with the lipophilic probe 1,1'-dihexadecyl-3,3,3',3'- tetramethylindocarbocyanine perchlorate (DiIC(16)) revealed a reticulated fluorescence pattern on the apical membrane. DiIC(16) was delivered as crystals to live cells to minimize possible solvent perturbations of the membrane lipids. The ratio of the integrated fluorescence ... More
Involvement of raft-like plasma membrane domains of Entamoeba histolytica in pinocytosis and adhesion.
AuthorsLaughlin RC, McGugan GC, Powell RR, Welter BH, Temesvari LA
JournalInfect Immun
PubMed ID15322032
'Lipid rafts are highly ordered, cholesterol-rich, and detergent-resistant microdomains found in the plasma membrane of many eukaryotic cells. These domains play important roles in endocytosis, secretion, and adhesion in a variety of cell types. The parasitic protozoan Entamoeba histolytica, the causative agent of amoebic dysentery, was determined to have raft-like ... More
Growth-associated protein-43 is required for commissural axon guidance in the developing vertebrate nervous system.
AuthorsShen Y, Mani S, Donovan SL, Schwob JE, Meiri KF
JournalJ Neurosci
PubMed ID11756507
'Growth-associated protein-43 (GAP-43) is a major growth cone protein whose phosphorylation by PKC in response to extracellular guidance cues can regulate F-actin behavior. Here we show that 100% of homozygote GAP-43 (-/-) mice failed to form the anterior commissure (AC), hippocampal commissure (HC), and corpus callosum (CC) in vivo. Instead, ... More
Use of lipophilic dyes in studies of axonal pathfinding in vivo.
AuthorsPerrin FE, Stoeckli ET
JournalMicrosc Res Tech
PubMed ID10620782
'Fluorescent lipophilic dyes are an ideal tool to study axonal pathfinding. Because these dyes do not require active axonal transport for their spreading, they can be used in fixed tissue. Here, we describe the method we have used to study the molecular mechanisms of commissural axon pathfinding in the embryonic ... More
Differential projections of ciliated and microvillous olfactory receptor cells in the catfish, Ictalurus punctatus.
AuthorsMorita Y, Finger TE
JournalJ Comp Neurol
PubMed ID9717708
'The primary olfactory projections of channel catfish Ictalurus punctatus have been examined with postmortem tracing by using either 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate or 1,1-dilinoleyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI). Following DiI deposition into small areas in different parts of the posterior olfactory bulb, olfactory sensory neurons always were labeled throughout the olfactory epithelium. No obvious ... More
Fluorescence methods to detect phase boundaries in lipid bilayer mixtures.
'Phase diagrams of lipid mixtures can show several different regions of phase coexistence, which include liquid-disordered, liquid-ordered, and gel phases. Some phase regions are small, and some have sharp boundaries. The identity of the phases, their location in composition space, and the nature of the transitions between the phases are ... More
Perforant path damage results in progressive neuronal death and somal atrophy in layer II of entorhinal cortex and functional impairment with increasing postdamage age.
AuthorsPeterson DA, Lucidi-Phillipi CA, Eagle KL, Gage FH
JournalJ Neurosci
PubMed ID7965084
'In vivo model systems that can evaluate neuronal death, survival, and regeneration are critical to revealing basic mechanisms of neuronal response and developing strategies for CNS repair. We propose a distinct experimental model of CNS degeneration following lesions to the perforant path connecting the hippocampus and the entorhinal cortex. Within ... More
Analysis of vertical fluorescence resonance energy transfer from the surface of a small-diameter sphere.
AuthorsJones GM, Wofsy C, Aurell C, Sklar LA
JournalBiophys J
PubMed ID9876165
'Fluorescence resonance energy transfer (FRET) measurements have been used to analyze fluorophore separations in a number of varying geometries, including small particles and extended surfaces. This study focuses on the geometry created by a donor extended above the surface of a small sphere (radius < R0), where the acceptors are ... More
Neuron-to-cell spread of pseudorabies virus in a compartmented neuronal culture system.
AuthorsCh'ng TH, Enquist LW,
JournalJ Virol
PubMed ID16103140
'Alphaherpesviruses are parasites of the peripheral nervous system in their natural hosts. After the initial infection of peripheral tissues such as mucosal cells, these neurotropic viruses will invade the peripheral nervous system that innervates the site of infection via long-distance axonal transport of the viral genome. In natural hosts, a ... More
Carbocyanine dyes with long alkyl side-chains: broad spectrum inhibitors of mitochondrial electron transport chain activity.
AuthorsAnderson WM, Trgovcich-Zacok D
JournalBiochem Pharmacol
PubMed ID7763312
'Certain indocarbocyanine, thiacarbocyanine, and oxacarbocyanine dyes possessing short alkyl side-chains (one to five carbons) are potent inhibitors of mammalian mitochondrial NADH-ubiquinone reductase (EC 1.6.99.3) activity (Anderson et al., Biochem Pharmacol 41: 677-684, 1991; Anderson et al., Biochem Pharmacol 45: 691-696, 1993; Anderson et al., Biochem Pharmacol 45: 2115-2122, 1993), and ... More
Neural tract tracing using Di-I: a review and a new method to make fast Di-I faster in human brain.
AuthorsSparks DL, Lue LF, Martin TA, Rogers J
JournalJ Neurosci Methods
PubMed ID11074091
'The use of Di-I in tract-tracing is briefly reviewed and a novel delayed-fixation approach to neural tract-tracing in the postmortem human adult brain is reported. Using the new approach, fast Di-I, a highly lipophilic fluorescent dye was injected into a particular region or nucleus and labelled tracts were followed for ... More
Sonic hedgehog guides commissural axons along the longitudinal axis of the spinal cord.
AuthorsBourikas D, Pekarik V, Baeriswyl T, Grunditz A, Sadhu R, Nardó M, Stoeckli ET
JournalNat Neurosci
PubMed ID15746914
'Dorsal commissural axons in the developing spinal cord cross the floor plate, then turn rostrally and grow along the longitudinal axis, close to the floor plate. We used a subtractive hybridization approach to identify guidance cues responsible for the rostral turn in chicken embryos. One of the candidates was the ... More
Persistent larval sensory neurones are required for the normal development of the adult sensory afferent projections in Drosophila.
AuthorsWilliams DW, Shepherd D
JournalDevelopment
PubMed ID11830563
We have tested the hypothesis that larval neurones guide growth of adult sensory axons in Drosophila. We show that ablation of larval sensory neurones causes defects in the central projections of adult sensory neurones. Spiralling axons and ectopic projections indicate failure in axon growth guidance. We show that larval sensory ... More
Specific projection of the sensory crypt cells in the olfactory system in crucian carp, Carassius carassius.
AuthorsHamdani el H, Døving KB,
JournalChem Senses
PubMed ID16306315
To study the projection of a special type of sensory neuron called crypt cells in the olfactory system in crucian carp, Carassius carassius, we applied the neural tracer 1,1-dilinoleyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) in the olfactory bulb (OB). Small crystals of DiI were applied in a small area at the synaptic region ... More
A direct interaction of axonin-1 with NgCAM-related cell adhesion molecule (NrCAM) results in guidance, but not growth of commissural axons.
AuthorsFitzli D, Stoeckli ET, Kunz S, Siribour K, Rader C, Kunz B, Kozlov SV, Buchstaller A, Lane RP, Suter DM, Dreyer WJ, Sonderegger P
JournalJ Cell Biol
PubMed ID10811834
An interaction of growth cone axonin-1 with the floor-plate NgCAM-related cell adhesion molecule (NrCAM) was shown to play a crucial role in commissural axon guidance across the midline of the spinal cord. We now provide evidence that axonin-1 mediates a guidance signal without promoting axon elongation. In an in vitro ... More
On-chip generation of microbubbles as a practical technology for manufacturing contrast agents for ultrasonic imaging.
AuthorsHettiarachchi K, Talu E, Longo ML, Dayton PA, Lee AP,
JournalLab Chip
PubMed ID17389962
This paper presents a new manufacturing method to generate monodisperse microbubble contrast agents with polydispersity index (sigma) values of <2% through microfluidic flow-focusing. Micron-sized lipid shell-based perfluorocarbon (PFC) gas microbubbles for use as ultrasound contrast agents were produced using this method. The poly(dimethylsiloxane) (PDMS)-based devices feature expanding nozzle geometry with ... More
Heat sensitization in skin and muscle nociceptors expressing distinct combinations of TRPV1 and TRPV2 protein.
AuthorsRau KK, Jiang N, Johnson RD, Cooper BY,
JournalJ Neurophysiol
PubMed ID17287441
Recordings were made from small and medium diameter dorsal root ganglia (DRG) neurons that expressed transient receptor potential (TRP) proteins. Physiologically characterized skin nociceptors expressed either TRPV1 (type 2) or TRPV2 (type 4) in isolation. Other nociceptors co-expressed both TRP proteins and innervated deep tissue sites (gastrocnemius muscle, distal colon; ... More
A proposal for a coordinated effort for the determination of brainwide neuroanatomical connectivity in model organisms at a mesoscopic scale.
AuthorsBohland JW, Wu C, Barbas H, Bokil H, Bota M, Breiter HC, Cline HT, Doyle JC, Freed PJ, Greenspan RJ, Haber SN, Hawrylycz M, Herrera DG, Hilgetag CC, Huang ZJ, Jones A, Jones EG, Karten HJ, Kleinfeld D, Kötter R, Lester HA, Lin JM, Mensh BD, Mikula S, Panksepp J, Price JL, Safdieh J, Saper CB, Schiff ND, Schmahmann JD, Stillman BW, Svoboda K, Swanson LW, Toga AW, Van Essen DC, Watson JD, Mitra PP,
JournalPLoS Comput Biol
PubMed ID19325892
In this era of complete genomes, our knowledge of neuroanatomical circuitry remains surprisingly sparse. Such knowledge is critical, however, for both basic and clinical research into brain function. Here we advocate for a concerted effort to fill this gap, through systematic, experimental mapping of neural circuits at a mesoscopic scale ... More
Distinct subpopulations of sensory afferents require F11 or axonin-1 for growth to their target layers within the spinal cord of the chick.
AuthorsPerrin FE, Rathjen FG, Stoeckli ET
JournalNeuron
PubMed ID11430805
Dorsal root ganglion neurons project axons to specific target layers in the gray matter of the spinal cord, according to their sensory modality. Using an in vivo approach, we demonstrate an involvement of the two immunoglobulin superfamily cell adhesion molecules axonin-1/TAG-1 and F11/F3/contactin in subpopulation-specific sensory axon guidance. Proprioceptive neurons, ... More
A map of pheromone receptor activation in the mammalian brain.
AuthorsBelluscio L, Koentges G, Axel R, Dulac C
JournalCell
PubMed ID10219242
In mammals, the detection of pheromones is mediated by the vomeronasal system. We have employed gene targeting to visualize the pattern of projections of axons from vomeronasal sensory neurons in the accessory olfactory bulb. Neurons expressing a specific receptor project to multiple glomeruli that reside within spatially restricted domains. The ... More
Relationship between oligodendrocytes and axons.
AuthorsBelichenko PV, Celio MR
JournalNeuroreport
PubMed ID9462475
Oligodendrocytes can myelinate a variable number of axons in their surroundings; however, the mechanisms underlying axon-oligodendrocyte associations are unknown. We tested the hypothesis that single oligodendrocytes exclusively myelinate axons belonging to the same functional system. Carbocyanine dyes (DiI, FAST DiI) were applied to the sternomastoid muscle of the rat and ... More
The tetraspanin CD81 is necessary for partitioning of coligated CD19/CD21-B cell antigen receptor complexes into signaling-active lipid rafts.
AuthorsCherukuri A, Shoham T, Sohn HW, Levy S, Brooks S, Carter R, Pierce SK
JournalJ Immunol
PubMed ID14688345
Tetraspanins have been hypothesized to facilitate the organization of functional multimolecular membrane complexes. In B cells the tetraspanin CD81 is a component of the CD19/CD21 complex. When coligated to the B cell Ag receptor (BCR), the CD19/CD21 complex significantly enhances BCR signaling in part by prolonging the association of the ... More
Dynamic redistribution of raft domains as an organizing platform for signaling during cell chemotaxis.
AuthorsGómez-Moutón C, Lacalle RA, Mira E, Jiménez-Baranda S, Barber DF, Carrera AC, Martínez-A C, Mañes S
JournalJ Cell Biol
PubMed ID14981096
Spatially restricted activation of signaling molecules governs critical aspects of cell migration; the mechanism by which this is achieved nonetheless remains unknown. Using time-lapse confocal microscopy, we analyzed dynamic redistribution of lipid rafts in chemoattractant-stimulated leukocytes expressing glycosyl phosphatidylinositol-anchored green fluorescent protein (GFP-GPI). Chemoattractants induced persistent GFP-GPI redistribution to the ... More
Ternary phase diagram of dipalmitoyl-PC/dilauroyl-PC/cholesterol: nanoscopic domain formation driven by cholesterol.
AuthorsFeigenson GW, Buboltz JT
JournalBiophys J
PubMed ID11371452
A ternary phase diagram is proposed for the hydrated lamellar lipid mixture dipalmitoylphosphatidylcholine/dilauroylphosphatidylcholine/cholesterol (DPPC/DLPC/cholesterol) at room temperature. The entire composition space has been thoroughly mapped by complementary experimental techniques, revealing interesting phase behavior that has not been previously described. Confocal fluorescence microscopy shows a regime of coexisting DPPC-rich ordered and ... More
Crosslinking a lipid raft component triggers liquid ordered-liquid disordered phase separation in model plasma membranes.
The mechanisms by which a cell uses and adapts its functional membrane organization are poorly understood and are the subject of ongoing investigation and discussion. Here, we study one proposed mechanism: the crosslinking of membrane components. In immune cell signaling (and other membrane-associated processes), a small change in the clustering ... More
Fluorescence resonance energy transfer analysis of lipopolysaccharide in detergent micelles.
AuthorsWiström CA, Jones GM, Tobias PS, Sklar LA
JournalBiophys J
PubMed ID8789116
Bacterial endotoxins or lipopolysaccharides (LPS), cell wall components of gram-negative bacteria, are involved in septic shock. LPS consists of a lipid A tail attached to core and O-antigen polysaccharides, but little is known about the supramolecular structure of LPS in blood. We have developed an approach to locate donor and ... More
Exogenous expression of N-cadherin in breast cancer cells induces cell migration, invasion, and metastasis.
AuthorsHazan RB, Phillips GR, Qiao RF, Norton L, Aaronson SA
JournalJ Cell Biol
PubMed ID10684258
E- and N-cadherin are calcium-dependent cell adhesion molecules that mediate cell-cell adhesion and also modulate cell migration and tumor invasiveness. The loss of E-cadherin-mediated adhesion has been shown to play an important role in the transition of epithelial tumors from a benign to an invasive state. However, recent evidence indicates ... More
Increased liver uptake of liposomes and improved targeting efficacy by labeling with asialofetuin in rodents.
AuthorsWu J, Liu P, Zhu JL, Maddukuri S, Zern MA
JournalHepatology
PubMed ID9500706
To improve liposome-directed therapy of liver disease and gene delivery, it would be beneficial to selectively target hepatocytes. For this purpose, conventional liposomes (CL) were labeled with asialofetuin (AF), an asialoglycoprotein. The biodistribution of AF-labeled liposomes (AF-L) in mice and their incorporation into rat hepatocytes, and their potential use in ... More
Endocytic sorting of lipid analogues differing solely in the chemistry of their hydrophobic tails.
AuthorsMukherjee S, Soe TT, Maxfield FR
JournalJ Cell Biol
PubMed ID10087269
To understand the mechanisms for endocytic sorting of lipids, we investigated the trafficking of three lipid-mimetic dialkylindocarbocyanine (DiI) derivatives, DiIC16(3) (1,1'-dihexadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate), DiIC12(3) (1,1'- didodecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate), and FAST DiI (1,1'-dilinoleyl-3,3,3', 3'-tetramethylindocarbocyanine perchlorate), in CHO cells by quantitative fluorescence microscopy. All three DiIs have the same head group, but differ in ... More
Progressive truncations C terminal to the membrane-spanning domain of simian immunodeficiency virus Env reduce fusogenicity and increase concentration dependence of Env for fusion.
AuthorsLin X, Derdeyn CA, Blumenthal R, West J, Hunter E
JournalJ Virol
PubMed ID12768026
The simian immunodeficiency virus (SIV) transmembrane (TM) protein, gp41, has multiple functions, which include anchoring the glycoprotein complex in the lipid envelope of the virus and mediating fusion of the virus and host cell membranes. Recently, a series of mutants of the SIVmac239 TM protein that have truncations at the ... More
Fc epsilon RI-mediated association of 6-micron beads with RBL-2H3 mast cells results in exclusion of signaling proteins from the forming phagosome and abrogation of normal downstream signaling.
AuthorsPierini L, Holowka D, Baird B
JournalJ Cell Biol
PubMed ID8830772
Cells of the mucosal mast cell line, RBL-2H3, are normally stimulated to degranulate after aggregation of high affinity receptors for IgE (Fc epsilon RI) by soluble cross-linking ligands. This cellular degranulation process requires sustained elevation of cytoplasmic Ca2+. In this study, we investigated the response of RBL-2H3 cells to 6-micron ... More
Effects of cholesterol depletion and increased lipid unsaturation on the properties of endocytic membranes.
AuthorsHao M, Mukherjee S, Sun Y, Maxfield FR
JournalJ Biol Chem
PubMed ID14734557
Lipid analogs with dialkylindocarbocyanine (DiI) head groups and short or unsaturated hydrocarbon chains (e.g. DiIC(12) and FAST DiI) enter the endocytic recycling compartment efficiently, whereas lipid analogs with long, saturated tails (e.g. DiIC(16) and DiIC(18)) are sorted out of this pathway and targeted to the late endosomes/lysosomes (Mukherjee, S., Soe, ... More
Localized sympathectomy reduces peripheral nerve regeneration and pain behaviors in 2 rat neuropathic pain models.
Authors
JournalPain
PubMed ID32701850
Polarity of the mouse embryo is established at blastocyst and is not prepatterned.
Authors
JournalGenes Dev
PubMed ID15879556
Temporal coherency between receptor expression, neural activity and AP-1-dependent transcription regulates Drosophila motoneuron dendrite development.
Authors
JournalDevelopment
PubMed ID23293292
The single AmphiTrk receptor highlights increased complexity of neurotrophin signalling in vertebrates and suggests an early role in developing sensory neuroepidermal cells.
Authors
JournalDevelopment
PubMed ID15799999
Zebrafish patient-derived xenograft models predict lymph node involvement and treatment outcome in non-small cell lung cancer.
Authors
JournalJ Exp Clin Cancer Res
PubMed ID35139880
Tentonin 3/TMEM150C senses blood pressure changes in the aortic arch.
Authors
JournalJ Clin Invest
PubMed ID32484458
TBX3 acts as tissue-specific component of the Wnt/β-catenin transcriptional complex.
Authors
JournalElife
PubMed ID32808927
Fulvestrant-Mediated Attenuation of the Innate Immune Response Decreases ER+ Breast Cancer Growth In Vivo More Effectively than Tamoxifen.
Authors
JournalCancer Res
PubMed ID32855207
Active Nerve Regeneration with Failed Target Reinnervation Drives Persistent Neuropathic Pain.
Authors
JournalEneuro
PubMed ID28197545
Biophysical experiments reveal a protective role of protein phosphatase Z1 against oxidative damage of the cell membrane in Candida albicans.