'Interactions among Bcl-2 family proteins mediated by Bcl-2 homology (BH) regions transform apoptosis signals into actions. The interactions between BH3 region-only proteins and multi-BH region proteins such as Bax and Bcl-2 have been proposed to be the dominant interactions required for initiating apoptosis. Experimental evidence also suggests that both homo- ... More
Membrane translocation of charged residues at the tips of hydrophobic helices in the T domain of diphtheria toxin.
AuthorsRen J, Sharpe JC, Collier RJ, London E
JournalBiochemistry
PubMed ID9893993
'The low pH triggered membrane insertion of the T domain of diphtheria toxin is a critical step in the translocation of the C domain of the toxin across membranes in vivo. We previously established that the T domain can interact with membranes in two distinct conformations, one in which the ... More
Improved retrograde axonal transport and subsequent visualization of tetramethylrhodamine (TMR) -dextran amine by means of an acidic injection vehicle and antibodies against TMR.
AuthorsKaneko T, Saeki K, Lee T, Mizuno N
JournalJ Neurosci Methods
PubMed ID8740593
'We studied the ability of various dextran amines (DA) to retrogradely label cortical neurons to the full extent of their dendritic configurations. Corticothalamic neurons were labeled by pressure injection of DA into the ventrobasal thalamic nuclei of the rat brain. Of fluorescein-, Texas Red-, Cascade Blue- and tetramethylrhodamine (TMR)-DAs of ... More
Intravital multiphoton microscopy of dynamic renal processes.
AuthorsMolitoris BA, Sandoval RM
JournalAm J Physiol Renal Physiol
PubMed ID15883167
'Recent advances in microscopy and optics, computer sciences, and the available fluorophores used to label molecules of interest have empowered investigators to utilize intravital two-photon microscopy to study the dynamic events within the functioning kidney. This emerging technique enables investigators to follow functional and structural alterations with subcellular resolution within ... More
Fast axonal diffusion of 3000 molecular weight dextran amines.
AuthorsFritzsch B
JournalJ Neurosci Methods
PubMed ID7506342
The distances of anterograde and retrograde axonal movement per hour were examined for dextran amines of 3000, 10,000 and 40,000 molecular weights (MW) conjugated to different fluorochromes (Cascade blue, fluorescein, tetramethylrhodamine, Texas red) or to biotin. Lateral line nerves of Xenopus laevis tadpoles were used as an easily accessible test ... More
The dynamin-like protein DLP1 is essential for normal distribution and morphology of the endoplasmic reticulum and mitochondria in mammalian cells.
AuthorsPitts KR, Yoon Y, Krueger EW, McNiven MA
JournalMol Biol Cell
PubMed ID10588666
The dynamin family of large GTPases has been implicated in vesicle formation from both the plasma membrane and various intracellular membrane compartments. The dynamin-like protein DLP1, recently identified in mammalian tissues, has been shown to be more closely related to the yeast dynamin proteins Vps1p and Dnm1p (42%) than to ... More
Diphtheria toxin forms pores of different sizes depending on its concentration in membranes: probable relationship to oligomerization.
AuthorsSharpe JC, London E
JournalJ Membr Biol
PubMed ID10501829
Diphtheria toxin forms pores in biological and model membranes upon exposure to low pH. These pores may play a critical role in the translocation of the A chain of the toxin into the cytoplasm. The effect of protein concentration on diphtheria toxin pore formation in model membrane systems was assayed ... More
Microfluidic Squeezing Enables MHC Class I Antigen Presentation by Diverse Immune Cells to Elicit CD8+ T Cell Responses with Antitumor Activity.
Authors
JournalJ Immunol
PubMed ID35091434
Maintenance of Mouse Gustatory Terminal Field Organization Is Disrupted following Selective Removal of Peripheral Sodium Salt Taste Activity at Adulthood.
Authors
JournalJ Neurosci
PubMed ID28676575
NFAT signaling in human mesenchymal stromal cells affects extracellular matrix remodeling and antifungal immune responses.
Authors
JournaliScience
PubMed ID34195564
Histological identification of phrenic afferent projections to the spinal cord.
Authors
JournalRespir Physiol Neurobiol
PubMed ID27838334
Fascin inhibitor increases intratumoral dendritic cell activation and anti-cancer immunity.
Authors
JournalCell Rep
PubMed ID33826900
Duodenum Intestine-Chip for preclinical drug assessment in a human relevant model.
Authors
JournalElife
PubMed ID31933478
Distribution and overlap of entorhinal, premotor, and amygdalar connections in the monkey anterior cingulate cortex.
Authors
JournalJ Comp Neurol
PubMed ID32677044
A Novel Microphysiological Colon Platform to Decipher Mechanisms Driving Human Intestinal Permeability.
Authors
JournalCell Mol Gastroenterol Hepatol
PubMed ID34284165
Culture of Intestinal Epithelial Cell Monolayers and Their Use in Multiplex Macromolecular Permeability Assays for In Vitro Analysis of Tight Junction Size Selectivity.