'It is uncertain how antiviral lymphocytes are activated in draining lymph nodes, the site where adaptive immune responses are initiated. Here, using intravital microscopy we show that after infection of mice with vaccinia virus (a large DNA virus) or vesicular stomatitis virus (a small RNA virus), virions drained to the ... More
Intravital two-photon microscopy for studying the uptake and trafficking of fluorescently conjugated molecules in live rodents.
AuthorsMasedunskas A, Weigert R,
JournalTraffic
PubMed ID18647170
'In this study, we describe an experimental system based on intravital two-photon microscopy for studying endocytosis in live animals. The rodent submandibular glands were chosen as model organs because they can be exposed easily, imaged without compromising their function and, furthermore, they are amenable to pharmacological and genetic manipulations. We ... More
T-cell priming by dendritic cells in lymph nodes occurs in three distinct phases.
AuthorsMempel TR, Henrickson SE, Von Andrian UH
JournalNature
PubMed ID14712275
'Primary T-cell responses in lymph nodes (LNs) require contact-dependent information exchange between T cells and dendritic cells (DCs). Because lymphocytes continually enter and leave normal LNs, the resident lymphocyte pool is composed of non-synchronized cells with different dwell times that display heterogeneous behaviour in mouse LNs in vitro. Here we ... More
Intracellular fluid flow in rapidly moving cells.
AuthorsKeren K, Yam PT, Kinkhabwala A, Mogilner A, Theriot JA,
JournalNat Cell Biol
PubMed ID19767741
'Cytosolic fluid dynamics have been implicated in cell motility because of the hydrodynamic forces they induce and because of their influence on transport of components of the actin machinery to the leading edge. To investigate the existence and the direction of fluid flow in rapidly moving cells, we introduced inert ... More
Vital staining of the hearing organ: visualization of cellular structure with confocal microscopy.
AuthorsFlock A, Scarfone E, Ulfendahl M
JournalNeuroscience
PubMed ID9466411
'Cells inside the intact organ of Corti were labelled with fluorescent probes reflecting various aspects of structure and function. The dyes were introduced into the perilymphatic space by perfusion of the scala tympani of the temporal bone from the guinea-pig maintained in isolation. The dyes were able to diffuse through ... More
Cell-free reconstitution of microautophagic vacuole invagination and vesicle formation.
AuthorsSattler T, Mayer A
JournalJ Cell Biol
PubMed ID11062255
'Many organelles change their shape in the course of the cell cycle or in response to environmental conditions. Lysosomes undergo drastic changes of shape during microautophagocytosis, which include the invagination of their boundary membrane and the subsequent scission of vesicles into the lumen of the organelle. The mechanism driving these ... More
In vivo multiphoton microscopy of deep brain tissue.
AuthorsLevene MJ, Dombeck DA, Kasischke KA, Molloy RP, Webb WW
JournalJ Neurophysiol
PubMed ID14668300
'Although fluorescence microscopy has proven to be one of the most powerful tools in biology, its application to the intact animal has been limited to imaging several hundred micrometers below the surface. The rest of the animal has eluded investigation at the microscopic level without excising tissue or performing extensive ... More
Intravital multiphoton microscopy of dynamic renal processes.
AuthorsMolitoris BA, Sandoval RM
JournalAm J Physiol Renal Physiol
PubMed ID15883167
'Recent advances in microscopy and optics, computer sciences, and the available fluorophores used to label molecules of interest have empowered investigators to utilize intravital two-photon microscopy to study the dynamic events within the functioning kidney. This emerging technique enables investigators to follow functional and structural alterations with subcellular resolution within ... More
Alterations in marginal zone macrophages and marginal zone B cells in old mice.
Marginal zones (MZs) are architecturally organized for clearance of and rapid response against blood-borne Ags entering the spleen. MZ macrophages (MZMs) and MZ B cells are particularly important in host defense against T-independent pathogens and may be crucial for the prevention of diseases, such as streptococcal pneumonia, that are devastating ... More
Functional studies of the kidney of living animals using multicolor two-photon microscopy.
AuthorsDunn KW, Sandoval RM, Kelly KJ, Dagher PC, Tanner GA, Atkinson SJ, Bacallao RL, Molitoris BA
JournalAm J Physiol Cell Physiol
PubMed ID12176747
Optical microscopy, when applied to living animals, provides a powerful means of studying cell biology in the most physiologically relevant setting. The ability of two-photon microscopy to collect optical sections deep into biological tissues has opened up the field of intravital microscopy to high-resolution studies of the brain, lens, skin, ... More
Annexin A2 supports pulmonary microvascular integrity by linking vascular endothelial cadherin and protein tyrosine phosphatases.
Authors
JournalJ Exp Med
PubMed ID28694388
Single-Cell Proteomics Reveals the Defined Heterogeneity of Resident Macrophages in White Adipose Tissue.
Authors
JournalFront Immunol
PubMed ID34381461
Transcytosis route mediates rapid delivery of intact antibodies to draining lymph nodes.
Authors
JournalJ Clin Invest
PubMed ID31232704
Yolk sac endoderm is the major source of serum proteins and lipids and is involved in the regulation of vascular integrity in early chick development.
Authors
JournalDev Dyn
PubMed ID21761483
Mapping Solute Clearance From the Mouse Hippocampus Using a 3D Imaging Cryomicrotome.
Authors
JournalFront Neurosci
PubMed ID33867918
Functional, structural, and molecular identification of lymphatic outflow from subconjunctival blebs.
Authors
JournalExp Eye Res
PubMed ID32387381
A Functional Tissue-Engineered Synovium Model to Study Osteoarthritis Progression and Treatment.
Authors
JournalTissue Eng Part A
PubMed ID30203722
Lysosome-mediated processing of chromatin in senescence.
Authors
JournalJ Cell Biol
PubMed ID23816621
Generation, localization and functions of macrophages during the development of testis.
Authors
JournalNat Commun
PubMed ID32873797
Dynamic Recruitment of Single RNAs to Processing Bodies Depends on RNA Functionality.