Search
Citations & References (24)
Invitrogen™
BODIPY™ FL C12-Sphingomyelin (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoyl) Sphingosyl Phosphocholine)
The green-fluorescent sphingomyelin, BODIPY™ FL C12-sphingomyelin can be used to study sphingolipid transport and signaling pathway in cells.Read more
| Catalog Number | Quantity |
|---|---|
| D7711 | 250 μL |
Catalog number D7711
Price (MXN)
-
Quantity:
250 μL
The green-fluorescent sphingomyelin, BODIPY™ FL C12-sphingomyelin can be used to study sphingolipid transport and signaling pathway in cells.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical Name or MaterialSphingolipids
Recommended StorageStore in freezer (-5 to -30°C) and protect from light.
Product LineBODIPY
Quantity250 μL
Unit SizeEach
Citations & References (24)
Citations & References
Abstract
Fluorescence-based selection of gene-corrected hematopoietic stem and progenitor cells from acid sphingomyelinase-deficient mice: implications for Niemann-Pick disease gene therapy and the development of improved stem cell gene transfer procedures.
Journal:Blood
PubMed ID:9864149
'The general utility of a novel, fluorescence-based procedure for assessing gene transfer and expression has been demonstrated using hematopoietic stem and progenitor cells. Lineage-depleted hematopoietic cells were isolated from the bone marrow or fetal livers of acid sphingomyelinase-deficient mice, and retrovirally transduced with amphotropic or ecotropic vectors encoding a normal
gamma-cyclodextrins greatly enhance translocation of hydrophobic fluorescent phospholipids from vesicles to cells in culture. Importance of molecular hydrophobicity in phospholipid trafficking studies.
Journal:J Biol Chem
PubMed ID:10585403
'Short-chain, fluorescent derivatives are commonly used to investigate intracellular phospholipid trafficking. However, their use can yield misleading results because they, unlike the native species, can rapidly distribute between organelles due to their low hydrophobicity. On the other hand, hydrophobic derivatives are very difficult to introduce to cells and thus have
A fluorescent glycolipid-binding peptide probe traces cholesterol dependent microdomain-derived trafficking pathways.
Journal:PLoS ONE
PubMed ID:18716682
'BACKGROUND: The uptake and intracellular trafficking of sphingolipids, which self-associate into plasma membrane microdomains, is associated with many pathological conditions, including viral and toxin infection, lipid storage disease, and neurodegenerative disease. However, the means available to label the trafficking pathways of sphingolipids in live cells are extremely limited. In order
A fluorescence-based, high-performance liquid chromatographic assay to determine acid sphingomyelinase activity and diagnose types A and B Niemann-Pick disease.
Journal:Anal Biochem
PubMed ID:12633609
'Acid sphingomyelinase (ASM; sphingomyelin phosphodiesterase, EC 3.1.4.12) is the lysosomal enzyme that hydrolyzes sphingomyelin (SPM) to phosphorylcholine and ceramide. An inherited deficiency of ASM activity results in Types A and B Niemann-Pick disease (NPD). In this study we report a new assay method to detect ASM activity and diagnose NPD
Synthesis of fluorescent substrates and their application to study of sphingolipid metabolism in vitro and in intact cells.
Journal:Methods Enzymol
PubMed ID:11070879