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DTNB; Ellman's Reagent, 5,5'-Dithiobis-(2-Nitrobenzoic Acid)

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DTNB or Elman's reagent can be used to quantitate thiols in proteins, cells and plasma by absorption measurements. It readily forms a mixed disulfide with thiols, liberating the chromophore 5-merapto-2-nitrobenzoic acid (absorption maximum 410 nm). Only protein thiols that are accessible to this water-soluble reagent are modified.
For Research Use Only. Not for use in diagnostic procedures.


Chemical Reactivity: Thiol
Label or Dye: DTNB
Product Size: 10 g
Shipping Condition: Room Temperature

Contents & storage

Store at room temperature.