Invitrogen™

EdU (5-ethynyl-2′-deoxyuridine)

Catalog number: E10187
Have Questions?
Invitrogen™

EdU (5-ethynyl-2′-deoxyuridine)

Catalog number: E10187
Have Questions?
Catalog Number
E10187
Unit Size
500 mg
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
50 mg
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
5 g
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
E10187500 mg
-
A1004450 mg
-
E104155 g
-
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EdU (5-ethynyl-2’-deoxyuridine) can be used as a replacement for BrdU (5-bromo-2’-deoxyuridine) and directly measures de novo DNA synthesis or S-phase synthesis of the cell cycle using click chemistry. Click chemistry is a method of covalently coupling an azide with an alkyne. Detection of EdU employs the copper(I) catalyzed click reaction with an azide modified fluorescent dye to form a stable triazole ring. Because of the small size of the click detection reagent, no harsh denaturation steps are needed to gain access to the DNA. Eliminating this step allows for more reproducible results, a simpler and quicker protocol and measurements which can easily be multiplexed with relevant antibody based targets including phospho-histone H3, Ki-67, and cyclin B1 as well as dual-pulse experiments with BrdU by flow cytometry, fluorescence microscopy, microplate (HTS) or high-throughput imaging (HCS).

EdU is readily soluble in DMSO, alcohol, water, or aqueous buffers. For use with in vitro applications, prepare 10 mM EdU stock solution in DMSO or aqueous buffer.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
Fluorescence
Format
Solid
Product Type
EdU
Shipping Condition
Room Temperature
Sample Type
Any Tissue Type, Cell Cultures, Tissue Sections, Cultured Cells, Whole Animals
Label or Dye
EdU
Quantity
500 mg
Product Line
Molecular Probes™, Click-iT™

Contents & Storage

Store at ≤-20°C.

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