Search
Citations & References (3)
Invitrogen™
EnzChek™ Phospholipase A1 Assay Kit
This kits takes our stand-alone assay for phospholipase A1, PLA1 (A10070) and combines the necessary reagents to run 2 toRead more
| Catalog Number | Quantity |
|---|---|
| E10219 | 200 Reactions kit |
Catalog number E10219
Price (MXN)
-
Quantity:
200 Reactions kit
This kits takes our stand-alone assay for phospholipase A1, PLA1 (A10070) and combines the necessary reagents to run 2 to 10 complete 96 well microplate assays that monitor activity in purified enzyme preparations and cell lysates. The importance of phospholipases in cellular signaling, lipid metabolism, inflammatory responses and pathological disorders related to these processes has stimulated demand for fluorescence-based activity monitoring methods. In particular the phospholipases resident in plasma and endothelium can perturb circulating LDL and HDL particles, creating pro-artherogenic forms. Recent evidence is drawing a link between these lipases and the progression of several severe neurodegenerative diseases, including Alzheimer's. Molecular Probes' fluorogenic phospholipase A1 substrates is designed to provide continuous monitoring of phospholipase A1 (PLA1) in purified enzyme preparations, cell lysates and living cells. PLA-1 improves upon two existing reagents in two ways: first it is now PLA-1 specific and secondly it has improved the assay quality by decreasing initial background noise.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence Intensity
Quantity200 Reactions kit
Shipping ConditionRoom Temperature
Substrate PropertiesLipid-Based Substrate, Chemical Substrate
Substrate TypePhospholipase Substrate
Target EnzymePhospholipase
For Use With (Application)Phospholipase Assay
Product LineEnzChek
Product TypeEnzChek Assay Kit
Unit SizeEach
Contents & Storage
1 kit. Store kit at -<20°C, protected from light, desiccated
Sufficient material is supplied for 200 reactions in 96 well microplates at 100 μl per well as described in the protocol below or 400 reaction in 384 low volume microplates at a volume of ≤ 25 μL per well.
Sufficient material is supplied for 200 reactions in 96 well microplates at 100 μl per well as described in the protocol below or 400 reaction in 384 low volume microplates at a volume of ≤ 25 μL per well.
Frequently asked questions (FAQs)
What is the LoD for the EnzChek Phospholipase A1 Assay Kit (Cat. No. E10219)?
Citations & References (3)
Citations & References
Abstract
Crystal structure of the predicted phospholipase LYPLAL1 reveals unexpected functional plasticity despite close relationship to acyl protein thioesterases.
Journal:J Lipid Res
PubMed ID:22052940
'Sequence homology indicates the existence of three human cytosolic acyl protein thioesterases, including APT1 that is known to depalmitoylate H- and N-Ras. One of them is the lysophospholipase-like 1 (LYPLAL1) protein that on the one hand is predicted to be closely related to APT1 but on the other hand might
Furin Is the Primary in Vivo Convertase of Angiopoietin-like 3 and Endothelial Lipase in Hepatocytes.
Journal:
PubMed ID:23918928
'The proprotein convertases (PCs) furin, PC5/6, and PACE4 exhibit unique and/or complementary functions. Their knock-out (KO) in mice resulted in strong and specific phenotypes demonstrating that, in vivo, these PCs are unique and essential during development. However, they also exhibit redundant functions. Liver angiopoietin-like 3 (ANGPTL3) inhibits lipolysis by binding
Measurement of the phospholipase activity of endothelial lipase in mouse plasma.
Journal:J Lipid Res
PubMed ID:23103358
Endothelial lipase (EL) is a major negative regulator of plasma HDL levels in mice, rabbits, and most probably, humans. Although this regulatory function is critically dependent on EL's hydrolysis of HDL phospholipids, as yet there is no phospholipase assay specific for EL in plasma. We developed such an assay for