Invitrogen™

EnzChek™ Phospholipase A1 Assay Kit

Catalog number: E10219
Invitrogen™

EnzChek™ Phospholipase A1 Assay Kit

Catalog number: E10219
Catalog Number
E10219
Unit Size
1 kit
Price (USD)
Price: 424.00
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
E102191 kit
Price: 424.00
Your price:
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This kits takes our stand-alone assay for phospholipase A1, PLA1 (A10070) and combines the necessary reagents to run 2 to 10 complete 96 well microplate assays that monitor activity in purified enzyme preparations and cell lysates. The importance of phospholipases in cellular signaling, lipid metabolism, inflammatory responses and pathological disorders related to these processes has stimulated demand for fluorescence-based activity monitoring methods. In particular the phospholipases resident in plasma and endothelium can perturb circulating LDL and HDL particles, creating pro-artherogenic forms. Recent evidence is drawing a link between these lipases and the progression of several severe neurodegenerative diseases, including Alzheimer's. Molecular Probes’ fluorogenic phospholipase A1 substrates is designed to provide continuous monitoring of phospholipase A1 (PLA1) in purified enzyme preparations, cell lysates and living cells. PLA-1 improves upon two existing reagents in two ways: first it is now PLA-1 specific and secondly it has improved the assay quality by decreasing initial background noise.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
Fluorescence Intensity
For Use With (Application)
Phospholipase Assay
Product Type
Phospholipase Assay
Product Line
EnzChek™, Molecular Probes™
Quantity
200 reactions, 200 Reactions
Shipping Condition
Room Temperature
Substrate Properties
Lipid-Based Substrate, Chemical Substrate
Substrate Type
Phospholipase Substrate
Target Enzyme
Phospholipase

Contents & Storage

1 kit. Store kit at -<20°C, protected from light, desiccated

Sufficient material is supplied for 200 reactions in 96 well microplates at 100 μl per well as described in the protocol below or 400 reaction in 384 low volume microplates at a volume of ≤ 25 μL per well.

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