EKMax™ is a recombinant preparation of the catalytic subunit of bovine enterokinase (1). EKMax™ recognizes the sequence Asp-Asp-Asp-Asp-Lys and cleaves the peptide bond after the lysine residue. The enzyme can be used to cleave any fusion protein that carries this peptide sequence (Figure 1).
Application: Removal of fusion tags from recombinant proteins.
Unit Definition: One unit of EKMax™ is the amount of enzyme required to digest 20 µg of a thioredoxin-CAT fusion protein to 90% completion in 16 hours at 37°C. One EKMax™ unit is equivalent to ~190 trypsinogen activation units.
For Research Use Only. Not for use in diagnostic procedures.
Contents & storage
250 or 1000 units of EKMax™ is provided with 10X buffer. Store at -20°C. Guaranteed stable for 6 months when stored properly.