The EnzChek™ Reverse Transcriptase Assay Kit is a convenient, efficient, and inexpensive assay for measuring reverse transcriptase activity. In less than an hour, samples can be read in a fluorometer or microplate reader with filter sets appropriate for fluorescein (FITC).

See our complete line of Fluorescence Microplate assays.

• Detect as little as 0.02 units of HIV-1 reverse transcriptase
• Large dynamic assay range, detect up to a 50-fold linear range
• Simple to use assay, amenable for automated high-throughput screening applications

The EnzChek™ Reverse Transcriptase Assay Kit uses PicoGreen™ reagent, which preferentially detects dsDNA or RNA-DNA heteroduplexes over single-stranded nucleic acids or free nucleotides. In this assay, the reverse transcriptase activity in a biological sample generates long RNA-DNA heteroduplexes from a mixture of a long poly(A) template, an oligo-dT primer, and dTTP. The RNA-DNA heteroduplexes formed are then detected by the PicoGreen™ reagent.