The EnzChek® Reverse Transcriptase Assay Kit is a convenient, efficient, and inexpensive assay for measuring reverse transcriptase activity. In less than an hour, samples can be read in a fluorometer or microplate reader with filter sets appropriate for fluorescein (FITC).
• Detect as little as 0.02 units of HIV-1 reverse transcriptase • Large dynamic assay range, detect up to a 50-fold linear range • Simple to use assay, amenable for automated high-throughput screening applications
The EnzChek® Reverse Transcriptase Assay Kit uses PicoGreen® reagent, which preferentially detects dsDNA or RNA-DNA heteroduplexes over single-stranded nucleic acids or free nucleotides. In this assay, the reverse transcriptase activity in a biological sample generates long RNA-DNA heteroduplexes from a mixture of a long poly(A) template, an oligo-dT primer, and dTTP. The RNA-DNA heteroduplexes formed are then detected by the PicoGreen® reagent.
For Research Use Only. Not for use in diagnostic procedures.
Reverse Transcriptase Assay
Contents & Storage
Upon receipt, the kit should be stored at -20°C, protected from light. The 20X TE buffer and the lambda DNA standard are best stored at 4°C; however, either may be frozen for long term storage. Stored properly, the kit's components should remain stable for at least six months. Allow reagents to warm to room temperature before opening the vials.