ER-Tracker™ Green (BODIPY™ FL Glibenclamide), for live-cell imaging

Zitierungen und Referenzen (28)

Invitrogen™

ER-Tracker™ Green (BODIPY™ FL Glibenclamide), for live-cell imaging

Der Farbstoff ER-Tracker™ Green ist ein zelldurchlässiges Färbemittel zur Anfärbung lebender Zellen und hochgradig selektiv für das endoplasmatische Retikulum (ER).Weitere Informationen

Katalognummer	Menge
E34251	100 μg

Katalognummer E34251

Preis (EUR)

687,65

Exklusiv online

724,00

Ersparnis 36,35 (5%)

Menge:

100 μg

Preis (EUR)

687,65

Exklusiv online

724,00

Ersparnis 36,35 (5%)

Der Farbstoff ER-Tracker™ Green ist ein zelldurchlässiges Färbemittel zur Anfärbung lebender Zellen und hochgradig selektiv für das endoplasmatische Retikulum (ER). Bei Färbung gemäß beiliegendem Protokoll bleibt das Färbemuster nach der Fixierung mit Formaldehyd teilweise erhalten. Dieses Färbemittel besteht aus dem grün fluoreszierenden BODIPY™ FL Farbstoff und Glibenclamid. Glibenclamid (Glyburid) bindet an die Sulfonylharnstoff-Rezeptoren der ATP-empfindlichen K⁺ Kanäle, die auf dem ER in großer Zahl vorhanden sind. Die pharmakologische Aktivität von Glibenclamid könnte die ER-Funktion beeinträchtigen. Die variable Expression der Sulfonylharnstoff-Rezeptoren in einigen spezialisierten Zelltypen kann zur Markierung außerhalb des ER führen.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

FarbeGrün, Grün

NachweisverfahrenFluoreszent, Fluoreszent

Zur Verwendung mit (Geräte)Fluoreszenzmikroskop, Fluoreszenzmikroskop

ProduktlinieBODIPY, ER-Tracker

Menge100 μg

VersandbedingungRaumtemperatur, Raumtemperatur

MarkertypBODIPY Farbstoffe

ProdukttypFarbstoff

SubCellular LocalizationEndoplasmatisches Retikulum

Unit SizeEach

Inhalt und Lagerung

Bei -5 bis -30 °C lagern und vor Licht schützen.

Häufig gestellte Fragen (FAQ)

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Zitierungen und Referenzen (28)

Zitierungen und Referenzen

Abstract

Morphological localisation of sulfonylurea receptor 1 in endocrine cells of human, mouse and rat pancreas.

Authors:Guiot Y, Stevens M, Marhfour I, Stiernet P, Mikhailov M, Ashcroft SJ, Rahier J, Henquin JC, Sempoux C,

Journal:Diabetologia

PubMed ID:17593344

'AIMS/HYPOTHESIS: Sulfonylurea receptor 1 (SUR1) is the regulatory subunit of ATP-sensitive K channels in beta cells. Morphological methods (immunohistochemistry and sulfonylurea binding) were used to establish the cellular and subcellular location of SUR1 in human and rodent islets. RESULTS: In the human, mouse and rat pancreas, all endocrine cells of ... More

Live intracellular super-resolution imaging using site-specific stains.

Authors:Carlini L, Manley S,

Journal:

PubMed ID:24079385

'Point localization super-resolution imaging (SR) requires dyes that can cycle between fluorescent and dark states, in order for their molecular positions to be localized and create a reconstructed image. Dyes should also densely decorate biological features of interest to fully reveal structures being imaged. We tested site-specific dyes in several ... More

Suicidal membrane repair regulates phosphatidylserine externalization during apoptosis.

Authors:Mirnikjoo B, Balasubramanian K, Schroit AJ,

Journal:J Biol Chem

PubMed ID:19561081

One of the hallmarks of apoptosis is the redistribution of phosphatidylserine (PS) from the inner-to-outer plasma membrane (PM) leaflet, where it functions as a ligand for phagocyte recognition and the suppression of inflammatory responses. The mechanism by which apoptotic cells externalize PS has been assumed to involve ... More

Mitochondrial autophagy is an HIF-1-dependent adaptive metabolic response to hypoxia.

Authors:Zhang H, Bosch-Marce M, Shimoda LA, Tan YS, Baek JH, Wesley JB, Gonzalez FJ, Semenza GL,

Journal:J Biol Chem

PubMed ID:18281291

Autophagy is a process by which cytoplasmic organelles can be catabolized either to remove defective structures or as a means of providing macromolecules for energy generation under conditions of nutrient starvation. In this study we demonstrate that mitochondrial autophagy is induced by hypoxia, that this process requires the hypoxia-dependent factor-1-dependent ... More

Translocation of a phycoerythrin alpha subunit across five biological membranes.

Authors:Gould SB, Fan E, Hempel F, Maier UG, Klösgen RB,

Journal:J Biol Chem

PubMed ID:17702756

Cryptophytes, unicellular algae, evolved by secondary endosymbiosis and contain plastids surrounded by four membranes. In contrast to cyanobacteria and red algae, their phycobiliproteins do not assemble into phycobilisomes and are located within the thylakoid lumen instead of the stroma. We identified two gene families encoding phycoerythrin alpha and light-harvesting complex ... More

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