Invitrogen™

Epidermal Growth Factor, Biotinylated, complexed to Alexa Fluor™ 555 Streptavidin (Alexa Fluor™ 555 EGF complex)

Catalog number: E35350
Invitrogen™

Epidermal Growth Factor, Biotinylated, complexed to Alexa Fluor™ 555 Streptavidin (Alexa Fluor™ 555 EGF complex)

Catalog number: E35350
Catalog Number
E35350
also known as E-35350
Unit Size
100 µg
Price (USD)
Price: 502.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
E35350
also known as E-35350
100 µg
Price: 502.00
Your Price:
-
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations
We offer several conjugates of epidermal growth factor that are useful for the detection of EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. The conjugates incorporating fluorescein (FITC), Oregon Green™ 514, and tetramethylrhodamine were constructed with the dye directly attached to EGF. The conjugates of EGF with Alexa Fluor™ dyes and Texas Red™ dye are complexes of the dye–streptavidin molecule and biotinylated EGF. The biotinylated conjugates utilize biotin-XX, which contains a long spacer arm designed to enhance the probe’s affinity for the EGF receptor.

Epidermal Growth Factor Conjugate Specifications:
• EGF molecule: 53 amino acids, MW=6,045 Da
• Label (Ex/Em): Biotin-XX complexed with Alexa Fluor™ 555 streptavidin (∼555/565 nm)
• Number of fluorophore molecules on each EGF molecule: ∼2–3
• Fluorescence is typically monitored using a flow cytometer, fluorescence microscope, or fluorimeter


Find More Receptor Binding and Phagocytosis Probes
We offer a number of fluorescently labeled probes for studying receptor-mediated endocytosis, membrane markers for endocytosis and exocytosis, and methods for detecting internalized fluorescent ligands. Review Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes™ Handbook for more information on these products.

For Research Use. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type
Mammalian Cells
Detection Method
Fluorescence
Form
Lyophilized
Product Type
Epidermal Growth Factor
Label or Dye
Alexa Fluor Dyes
Product Line
Alexa Fluor™
Shipping Condition
Wet Ice

Contents & Storage

Store in freezer (-5 to -30°C) and protect from light.