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Citations et références (52)
Invitrogen™
Ethidium Homodimer-2 (EthD-2) - 1 mM Solution in DMSO
L’indicateur de viabilité imperméant aux cellules homodimère-2 d’éthidium (EthD-2) est une coloration d’acide nucléique de haute affinité qui est faiblementAfficher plus
| Référence | Quantité |
|---|---|
| E3599 | 200 μl |
Référence E3599
Prix (EUR)
728,00
Each
Quantité:
200 μl
Prix (EUR)
728,00
Each
L’indicateur de viabilité imperméant aux cellules homodimère-2 d’éthidium (EthD-2) est une coloration d’acide nucléique de haute affinité qui est faiblement fluorescente jusqu’à sa liaison avec l’ADN et émet une fluorescence rouge (maxima d’excitation / émission de ∼ 535 / 624).
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
CouleurOrange, Orange
DescriptionHomodimère-2 d’éthidium (EthD-2) - Solution de 1 mM dans du DMSO
Méthode de détectionFluorescence, Fluorescent
Type de colorantImperméant aux cellules
Émission624 nm
Gamme de longueur d’onde d’excitation535 nm
À utiliser avec (application)Dosages de coloration cellulaire
À utiliser avec (équipement)Microscope à fluorescence, Microscope à fluorescence
Quantité200 μl
Conditions d’expéditionTempérature ambiante
Type d’étiquetteFluorescent Dye
Type de produitHomodimère-2 d’éthidium
SubCellular LocalizationNoyau, acides nucléiques, Nucleus
Unit SizeEach
Contenu et stockage
Conserver au congélateur (entre -5°C et -30°C) et à l’abri de la lumière.
Citations et références (52)
Citations et références
Abstract
Differential effects of deuterium oxide on the fluorescence lifetimes and intensities of dyes with different modes of binding to DNA.
Journal:J Histochem Cytochem
PubMed ID:9016307
'Deuterium oxide (D2O) increases both the fluorescence lifetime and the fluorescence intensity of the intercalating dyes propidium iodide (PI) and ethidium bromide (EB) when bound to nucleic acid structures. We have used spectroscopic analysis coupled with conventional and phase-sensitive flow cytometry to compare the alterations in intensity and lifetime of
Diverse microglial motility behaviors during clearance of dead cells in hippocampal slices.
Journal:Glia
PubMed ID:15042586
'We used two-channel three-dimensional time-lapse fluorescence confocal imaging in live rat hippocampal slice cultures (1-7 days in vitro) to determine the motility behaviors of activated microglia as they engage dead and dying cells following traumatic brain tissue injury. Live microglia were labeled with a fluorescently conjugated lectin (IB(4)), and dead
Calmodulin antagonists differentially affect capacitation-associated protein tyrosine phosphorylation of mouse sperm components.
Journal:J Cell Sci
PubMed ID:12668727
'Sperm capacitation in vitro is thought to be correlated with the increased protein tyrosine phosphorylation of a subset of sperm components. Our group recently used a pharmacological approach to demonstrate that calmodulin (CaM), a 17 kDa calcium sensor protein, has a role in sperm capacitation. In the present study, we
Automated image analysis of live/dead staining of the fungus Aureobasidium pullulans on microscope slides and leaf surfaces.
Journal:Biotechniques
PubMed ID:11056819
'An image analysis program and protocol for the identification and enumeration of live versus dead cells of the yeast-like fungus Aureobasidium pullulans was developed for both populations on microscope slides and leaf surfaces. Live cells took up CellTracker Blue, while nonviable cells stained with DEAD Red. Image analysis macro programs
A2E-epoxides damage DNA in retinal pigment epithelial cells. Vitamin E and other antioxidants inhibit A2E-epoxide formation.
Journal:J Biol Chem
PubMed ID:12646558
'The autofluorescent pigments that accumulate in retinal pigment epithelial cells with aging and in some retinal disorders have been implicated in the etiology of macular degeneration. The major constituent is the fluorophore A2E, a pyridinium bisretinoid. Light-exposed A2E-laden retinal pigment epithelium exhibits a propensity for apoptosis with light in the