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Citations & References (38)
Invitrogen™
ELF™ 97 Phosphatase Substrate (ELF™ 97 Phosphate), 0.2 μm Filtered
The ELF™ 97 phosphatase substrate upon hydrolysis produces a bright and photostable yellow-green fluorescent precipitate at the site of enzymaticRead more
| Catalog Number | Quantity |
|---|---|
| E6588 | 1 mL |
Catalog number E6588
Price (KRW)
658,000
Online offer
Ends: 31-Dec-2025
773,000Save 115,000 (15%)
Each
Quantity:
1 mL
Price (KRW)
658,000
Online offer
Ends: 31-Dec-2025
773,000Save 115,000 (15%)
Each
The ELF™ 97 phosphatase substrate upon hydrolysis produces a bright and photostable yellow-green fluorescent precipitate at the site of enzymatic activity. This fluorescent precipitate has several unique spectral characteristics, including an extremely large Stokes shift that makes it easily distinguishable from endogenous fluorescence.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell PermeabilityCell-Permeant
ColorYellow-Green
Concentration5 mM
Excitation/Emission345⁄530
For Use With (Equipment)Fluorescence Microscope, Microarray, Flow Cytometer
Label or DyeELF 97
Product LineELF
Quantity1 mL
Research CategoryDifferentiation
Shipping ConditionRoom Temperature
SubstratePhosphatase Substrate
Detection MethodFluorescence
FormLiquid
Substrate PropertiesChemical Substrate
Target EnzymePhosphatases
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.
Frequently asked questions (FAQs)
Can the ELF 97 reagent be applied to live cells?
Can ELF 97 stained cell/tissue samples be multiplexed with antibody labeling?
Citations & References (38)
Citations & References
Abstract
Characterization of a new NIH-registered variant human embryonic stem cell line, BG01V: a tool for human embryonic stem cell research.
Journal:Stem cells (Dayton, Ohio)
PubMed ID:16293579
Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research.
Journal:Stem Cells
PubMed ID:17284651
'As the number of human embryonic stem cell (hESC) lines increases, so does the need for systematic evaluation of each line''s characteristics and potential. Comparisons between lines are complicated by variations in culture conditions, feeders, spontaneous differentiation, and the absence of standardized assays. These difficulties, combined with the inability of
Quantitative differences in phase I and II metabolism between rat precision-cut liver slices and isolated hepatocytes.
Journal:Drug Metab Dispos
PubMed ID:8591730
'Testosterone (250 microM), 7-ethoxycoumarin (25 microM), and 1-chloro-2,4-dinitrobenzene (CDNB, 50 microM) were used as substrates to compare phase I and II metabolism in rat precision-cut liver slices and rat hepatocytes. Overall clearance to metabolites was significantly greater in hepatocytes for testosterone (1.9- to 16.9-fold), 7-ethoxycoumarin (O-deethylation, 14.8-fold; glucuronidation, 3.1-fold), and
Alkaline phosphatase is involved in the control of adipogenesis in the murine preadipocyte cell line, 3T3-L1.
Journal:Clin Chim Acta
PubMed ID:15748605
'OBJECTIVE: As alkaline phosphatase may play a role in cell differentiation, our aim was to study the possible role of this enzyme in the differentiation of preadipocytes (3T3-L1 cells) into adipocytes. RESEARCH METHODS AND PROCEDURES: 3T3-L1 cells were grown in medium containing insulin, dexamethasone and IBMX to induce adipogenesis. Adipogenesis
Fluorescence in situ hybridization of scarce leptin receptor mRNA using the enzyme-labeled fluorescent substrate method and tyramide signal amplification.
Journal:J Histochem Cytochem
PubMed ID:11101627
'To increase the sensitivity of fluorescence in situ hybridization (FISH) for detection of low-abundance mRNAs, we performed FISH on cryostat sections of rat hypothalamus with biotin-labeled riboprobes to leptin receptor (ObRb) and amplified the signal by combining tyramide signal amplification (TSA) and Enzyme-Labeled Fluorescent alkaline phosphatase substrate (ELF) methods. First,