Detect and measure enzyme activity with EnzChek protease assay kits »

Citations & References (19)

Invitrogen™

EnzChek™ Protease Assay Kit, green fluorescence, 100-1000 assays

Continuously measure protease kinetics

Catalog Number	Quantity
E6638	1 Kit

Catalog number E6638

Price (USD)

570.00

Quantity:

1 Kit

Price (USD)

570.00

The EnzChek™ Protease Assay Kit, green fluorescence, is a fast, simple, and direct fluorescence-based assay for detecting metallo-, serine, acid, and sulfhydryl proteases. The accompanying increase in fluorescence, which can be measured with fluorescence microplate reader, is proportional to protease activity.

Generalized mechanism allows for measurement of a variety of proteases
Can be used to continuously measure protease kinetics
Excitation/emission of 505/513 nm allows use of standard fluorescein (FITC) filter settings

This EnzChek™ Protease Assay Kit contains a casein derivative that has been extensively labeled with the pH-insensitive, green-fluorescent BODIPY™ FL dye, which results in a quenching of the fluorescent dye. Protease-catalyzed hydrolysis releases the highly-fluorescent BODIPY™ FL dye-labeled peptides, allowing for quantitative detection of protease activity in solution. The green-fluorescent BODIPY™ FL dye has excitation and emission spectra similar to those of fluorescein.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection MethodFluorescence

Emission Wavelength513 nm

Excitation Wavelength505 nm

Quantity1 Kit

Research CategoryDifferentiation

Shipping ConditionRoom Temperature

Substrate PropertiesProtein-Based Substrate

Substrate TypeProtease Substrate

Target EnzymeProteases

For Use With (Application)Enzyme and Metabolic Activity

For Use With (Equipment)Microplate Reader

Product LineEnzChek

Product TypeEnzChek Assay Kit

Unit SizeEach

Contents & Storage

Store in freezer -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

What is the optimal pH range for protease activity detection using the EnzChek Protease Assay Kit?

The digestion buffer provided in the kit (pH 7.8) is recommended for detecting the protease activity of most proteolytic enzymes with activity optima from pH 7.4 to 8.0. However, if you are working with an enzyme that requires activation compounds or a unique pH environment, then prepare the specific buffer required in place of the digestion buffer.

What is the shelf life for EnzChek Protease Assay kits (Cat. Nos. E6638, E6639)?

As stated in the manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/mp06638.pdf) on page 2 under section "Storage and Handling", these kits are stable for 6-12 months if stored as indicated.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Citations & References (19)

Citations & References

Abstract

Authors:

Journal:

PubMed ID:10748021

Effective antiprotease-antibiotic treatment of experimental anthrax.

Authors:Popov SG, Popova TG, Hopkins S, Weinstein RS, MacAfee R, Fryxell KJ, Chandhoke V, Bailey C, Alibek K

Journal:BMC Infect Dis

PubMed ID:15819985

'BACKGROUND: Inhalation anthrax is characterized by a systemic spread of the challenge agent, Bacillus anthracis. It causes severe damage, including multiple hemorrhagic lesions, to host tissues and organs. It is widely believed that anthrax lethal toxin secreted by proliferating bacteria is a major cause of death, however, the pathology of ... More

Enzymatic microreactor-on-a-chip: protein mapping using trypsin immobilized on porous polymer monoliths molded in channels of microfluidic devices.

Authors:Peterson DS, Rohr T, Svec F, Fréchet JM

Journal:Anal Chem

PubMed ID:12199578

'Enzymatic microreactors have been prepared in capillaries and on microfluidic chips by immobilizing trypsin on porous polymer monoliths consisting of 2-vinyl-4,4-dimethylazlactone, ethylene dimethacrylate, and acrylamide or 2-hydroxyethyl methacrylate. The azlactone functionalities react readily with amine and thiol groups of the enzyme to form stable covalent bonds. The optimized porous properties ... More

Quenched BODIPY dye-labeled casein substrates for the assay of protease activity by direct fluorescence measurement.

Authors:Jones LJ, Upson RH, Haugland RP, Panchuk-Voloshina N, Zhou M, Haugland RP

Journal:Anal Biochem

PubMed ID:9299009

'We have prepared casein conjugates of two BODIPY dyes for use as fluorogenic protease substrates in homogeneous assays. Both conjugates are labeled to such an extent that the dyes are efficiently quenched in the protein, yielding virtually nonfluorescent substrate molecules. These fluorogenic substrates release highly fluorescent BODIPY dye-labeled peptides upon ... More

Functional screening of intracellular proteins in single cells and in patterned cell arrays using electroporation.

Authors:Nolkrantz K, Farre C, Hurtig KJ, Rylander P, Orwar O

Journal:Anal Chem

PubMed ID:12199607

'A tool for detection and characterization of intracellular enzyme-substrate and receptor-ligand interactions inside the cytoplasm of single targeted cells or small confined groups of cells is presented. Fluorogenic enzyme substrates and receptor ligands were rapidly delivered by electroosmosis and internalized by electroporation in cells using an electrolyte-filled capillary (EFC) biased ... More

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