EnzChek® Protease Assay Kit, green fluorescence, 100-1000 assays

Catalog number: E6638

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function | Protein Assays and Analysis | Stem Cell Analysis | Stem Cell Differentiation

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Description

The EnzChek® Protease Assay Kit, green fluorescence, is a fast, simple, and direct fluorescence-based assay for detecting metallo-, serine, acid, and sulfhydryl proteases. The accompanying increase in fluorescence, which can be measured with fluorescence microplate reader, is proportional to protease activity.

See our complete line of Fluorescence Microplate assays.

• Generalized mechanism allows for measurement of a variety of proteases
• Can be used to continuously measure protease kinetics
• Excitation/emission of 505/513 nm allows use of standard fluorescein (FITC) filter settings

This EnzChek® Protease Assay Kit contains a casein derivative that has been extensively labeled with the pH-insensitive, green-fluorescent BODIPY® FL dye, which results in a quenching of the fluorescent dye. Protease-catalyzed hydrolysis releases the highly-fluorescent BODIPY® FL dye-labeled peptides, allowing for quantitative detection of protease activity in solution. The green-fluorescent BODIPY® FL dye has excitation and emission spectra similar to those of fluorescein.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Technique: Fluorescence Intensity
Assay Type: Protease Assay
Product Size: 1 kit
Target Enzyme: Proteases
Substrate Type: Protease Substrate
Substrate Properties: Protein-Based Substrate
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.

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Manuals & protocols

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