The EnzChek™ Protease Assay Kit, red fluorescence, is a fast, simple, and direct fluorescence-based assay for detecting metallo-, serine, acid, and sulfhydryl proteases. The accompanying increase in fluorescence, which can be measured with a fluorescence microplate reader, is proportional to protease activity.

See our complete line of Fluorescence Microplate assays.

• Generalized mechanism allows for measurement of a variety of proteases
• Can be used to continuously measure protease kinetics
• Excitation/emission similar to Texas Red™ dye (589/617 nm)

This EnzChek™ Protease Assay Kit contains a casein derivative that has been extensively labeled with the red-fluorescent BODIPY™ TR-X dye, which results in a quenching of the fluorescent dye. Protease-catalyzed hydrolysis releases the highly-fluorescent BODIPY™ TR-X dye-labeled peptides, allowing for quantitative detection of protease activity in solution. The red-fluorescent BODIPY™ TR-X dye has excitation and emission spectra similar to those of the Texas Red™ fluorophore.