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Invitrogen™
EnzChek™ Protease Assay Kit, red fluorescence, 100-1000 assays
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Description
The EnzChek® Protease Assay Kit, red fluorescence, is a fast, simple, and direct fluorescence-based assay for detecting metallo-, serine, acid, and sulfhydryl proteases. The accompanying increase in fluorescence, which can be measured with a fluorescence microplate reader, is proportional to protease activity.See our complete line of Fluorescence Microplate assays.
• Generalized mechanism allows for measurement of a variety of proteases
• Can be used to continuously measure protease kinetics
• Excitation/emission similar to Texas Red® dye (589/617 nm)
This EnzChek® Protease Assay Kit contains a casein derivative that has been extensively labeled with the red-fluorescent BODIPY® TR-X dye, which results in a quenching of the fluorescent dye. Protease-catalyzed hydrolysis releases the highly-fluorescent BODIPY® TR-X dye-labeled peptides, allowing for quantitative detection of protease activity in solution. The red-fluorescent BODIPY® TR-X dye has excitation and emission spectra similar to those of the Texas Red® fluorophore.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Assay Type: | Protease Assay |
|---|---|
| Product Line: | EnzChek® |
| Product Size: | 1 kit |
| Substrate Properties: | Protein-Based Substrate |
| Substrate Type: | Protease Substrate |
| Target Enzyme: | Proteases |
| Technique: | Fluorescence Intensity |
| Shipping Condition: | Room Temperature |
Contents & storage
Store in freezer (-5 to -30°C) and protect from light.
