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      Invitrogen™

      EnzChek™ Protease Assay Kit, red fluorescence, 100-1000 assays

      Catalog number:  E6639

       Related applications: Protein Assays and Analysis

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      Description

      The EnzChek® Protease Assay Kit, red fluorescence, is a fast, simple, and direct fluorescence-based assay for detecting metallo-, serine, acid, and sulfhydryl proteases. The accompanying increase in fluorescence, which can be measured with a fluorescence microplate reader, is proportional to protease activity.

      See our complete line of Fluorescence Microplate assays.

      • Generalized mechanism allows for measurement of a variety of proteases
      • Can be used to continuously measure protease kinetics
      • Excitation/emission similar to Texas Red® dye (589/617 nm)

      This EnzChek® Protease Assay Kit contains a casein derivative that has been extensively labeled with the red-fluorescent BODIPY® TR-X dye, which results in a quenching of the fluorescent dye. Protease-catalyzed hydrolysis releases the highly-fluorescent BODIPY® TR-X dye-labeled peptides, allowing for quantitative detection of protease activity in solution. The red-fluorescent BODIPY® TR-X dye has excitation and emission spectra similar to those of the Texas Red® fluorophore.
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Assay Type: Protease Assay
      Product Line: EnzChek®
      Product Size: 1 kit
      Substrate Properties: Protein-Based Substrate
      Substrate Type: Protease Substrate
      Target Enzyme: Proteases
      Technique: Fluorescence Intensity
      Shipping Condition: Room Temperature

      Contents & storage

      Store in freezer (-5 to -30°C) and protect from light.

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