Catalog Number
E6646
Unit Size
100 assays
Price (USD)
428.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
E6646100 assays
428.00
Your Price:
***
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The EnzChek™ Phosphate Assay Kit provides a fast and sensitive spectrophotometric method for the quantification of inorganic phosphate in solution and for phosphate released during ATPase or GTPase enzymatic reactions.

See our complete line of Fluorescence Microplate assays.

• Sensitivity of 2 to 150 µM phosphate
• Spectrophotometric microplate-based assay detects a change in absorbance maximum from 330 nm to 360 nm
• Continuous monitoring of phosphate generated by ATPase or GTPase activity

In the presence of inorganic phosphate, the 2-amino-6-mercapto-7-methylpurine riboside (MESG) substrate is converted by the purine nucleoside phosphorylase (PNP) enzyme to the ribose 1-phosphate and 2-amino-6-mercapto-7-methylpurine product. This enzymatic conversion of MESG results in a spectrophotometric shift in maximum absorbance from 330 nm for the substrate to 360 nm for the product.

Sensitivity of the assay is in the range of 2 to 150 µM inorganic phosphate (2 to 150 nanomoles phosphate in a 1 mL volume), and the reaction can be performed over a pH range of 6.5 to 8.5. The EnzChek™ phosphate reaction is sufficiently fast and quantitative for use in stopped-flow kinetic experiments.

Specifications

Color
Ultraviolet
Detection Method
Colorimetric
For Use With (Application)
Phosphate Assay
For Use With (Equipment)
Spectrophotometer
Product Type
Phosphate Assay
Dye Type
MESG
Format
Tube(s)
Product Line
EnzChek™
Shipping Condition
Room Temperature

Contents & Storage

Store in freezer -5°C to -30°C.

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