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Thermo Scientific™

DH5α Competent Cells

Catalog number: EC0112
Thermo Scientific™

DH5α Competent Cells

Catalog number: EC0112
Catalog Number
EC0112
Unit Size
10 x 100 µL
Price (USD)
Price: 152.00
Online price: 135.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
EC011210 x 100 µL
Price: 152.00
Online price: 135.65
Your price:
-
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Thermo Scientific DH5α Competent Cells are high efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors. The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal. RecA1 and endA1 mutations in DH5α cells improve insert stability and quality of the extracted plasmid DNA.

• High transformation efficiency: >1x109 cfu/μg pUC19 DNA
• Suitable for routine and high-throughput cloning applications
• Genetic markers that allow for blue/white colony screening
• Convenient two reactions per vial packaging
• S.O.C. Outgrowth medium included

Applications
DH5α Competent Cells are suitable for variety of applications requiring high transformation efficiency:
• Efficient DNA cloning derived from PCR, cDNA-generation reactions
• recA1 marker facilitates working with difficult to transform DNA
• Ideal for generation of cDNA libraries using plasmid-derived vectors
• Site-directed mutagenesis

Genotype
F– φ80lacZΔ M15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 λ- thi–1 gyrA96 relA1
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Competent Cell
Contains F' Episome
Lacks F' Episome
Efficiency
High Efficiency (>1x10^9 cfu/μg pUC19 DNA)
Cell Type
Chemically Competent
Libraries
cDNA
Cloning Methylated DNA
No
Bacterial or Yeast Strain
DH5α
Blue/White Screening
Yes
High-throughput Compatibility
Not High-throughput Compatible
Propagating ccdB Vectors
Not for ccdB vector propagation
Shipping Condition
Dry Ice
Species
E. coli
Format
Tube
Product Line
Thermo Scientific™
For Use With (Application)
Cloning
Quantity
10 x 100 μL

Contents & Storage

• 10 x DH5α Competent Cells (100 μL)
• pUC19 DNA (50 μL) (10 pg/μL)
• S.O.C. Medium (5 mL)

Store at -80°C.

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