2D electrophoresis is used to separate proteins based upon their molecular weight after isoelectric focusing (IEF). 2D gels are generally designed to accept IEF tube gels, sample lanes sliced from vertical or horizontal IEF slab gels, or immobilized pH gradient (IPG) strips. Gels are usually run under denaturing conditions using SDS in the gel running buffers. ZOOM™ Gels are specifically designed for 2D separation of proteins that have been first separated by their pI on 7-cm IPG strips (e.g., ZOOM™ Strips). The IPG well of ZOOM™ Gels accommodates the full length of a 7-cm IPG strip (Figure 1). In addition to the IPG well, ZOOM™ Gels have a separate well designed for use with a molecular weight standard. The ZOOM™ cassette is the standard 10 cm x 10 cm format. ZOOM™ Gels are available in NuPAGE™ and Novex™ Tris-Glycine gel formulations.
For Research Use Only. Not for use in diagnostic procedures.