Are there any suggestions on how to reduce high background when using the Colloidal Blue Staining Kit on Invitrogen Tris-Glycine gels?
It may be possible to reduce background by using the protocol given for NuPAGE Invitrogen Bis-Tris/Small Peptides.
This protocol incorporates an extra fix step to remove excess SDS, which can act as an anti-colloidal agent and lead to higher background.
The low pH of the staining solution will fix the gel, but not as fast as the pre-fix step specified in the NuPAGE protocol.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
This protocol incorporates an extra fix step to remove excess SDS, which can act as an anti-colloidal agent and lead to higher background.
The low pH of the staining solution will fix the gel, but not as fast as the pre-fix step specified in the NuPAGE protocol.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.