Novex™ TBE Gels, 20%

Citations & References (15)

Invitrogen™

Novex™ TBE Gels, 20%

Name: Novex™ TBE Gels, 20%
SKU: EC6315BOX

Novex™ TBE Gels 20% designed to run on the XCell SureLock™ Mini-Cell provide high-resolution analysis of restriction digests and PCR products.

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Catalog Number	Wells
EC6315BOX	10-well
EC63152BOX	12-well
EC63155BOX	15-well

3 Options

Catalog number EC6315BOX

Price (MXN)

Wells:

10-well

Request bulk or custom format

Novex™ TBE Gels 20% provide high-resolution analysis of restriction digests and PCR products. Designed to run on the XCell SureLock™ Mini-Cell, the polyacrylamide gels give sharp, clearly resolved, intense bands, and provide separations of double-strand DNA fragments from 15–50 bp.

Novex TBE Gels are available in a variety of well formats and gel percentages and can be stained by silver staining, ethidium bromide, and SYBR™ Green staining techniques after electrophoresis.

Advantages of TBE Gels for nucleic acid separation:
• High resolution and sensitivity with lower background staining
• Requires ∼10% sample concentration and volume of large or agarose gels
• Efficient blotting
• Easy extraction of DNA from gels
• Gel extraction does not interfere with enzymatic reactions
• Accurate and reproducible results

Formulation
Novex TBE gels are manufactured with high-purity Tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEMED, and APS.

Recommended buffers
For optimum performance, Novex™ TBE Running Buffer and Novex™ Hi-Density TBE Sample Buffer are strongly recommended for use with these gels. Novex Hi-Density TBE Sample Buffer contains the tracking dyes Bromophenol Blue and Xylene Cyanol as well as the density agent Ficoll™, which yields sharper and straighter bands than conventional density agents.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

DescriptionNovex TBE Gels, 20%, 10-well

Product TypeTBE Gel

Quantity10 Gels/Box

Sample TypeDouble-stranded DNA (dsDNA)

Shipping ConditionWet Ice

For Use With (Equipment)XCell SureLock Mini-Cell

Gel Percentage20%

Gel TypeTBE

Separation Range15 to 50 bp

Wells10-well

Unit SizeEach

Contents & Storage

• Catalog number refers to a single box of 10 gels

Store at 4°C.

Frequently asked questions (FAQs)

Can I stain my TBE gel? How?

Yes, you can stain your TBE gels with ethidium bromide, SYBR Green I, SYBR Green II, and the SilverXpress Silver Staining Kit. For ethidium bromide staining, soak the gel in a 2 µg/mL solution of ethidium bromide in ultrapure water for 20 minutes. Destain by rinsing with three successive 10-minute rinses of ultrapure water. Visualize bands under UV light.

What are the smallest fragments that can be visualized on the TBE gels?

On the Invitrogen 10% TBE gels, a 51 bp marker can be clearly seen. On the Invitrogen 20% TBE gel, the 18 and 12 bp markers can be clearly seen.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What is the concentration of EDTA in the TBE gels? How about glycerol?

The EDTA concentration in our TBE gels is 0.06% (w/v). The 20% TBE gels contain 4% glycerol for maximal resolution. All other TBE gels contain 0.8% glycerol in a layer that represents the bottom 9% of the gel. There is no glycerol in the rest of the gel.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Can I stain my TBE gel or my TBE-urea gel? How?

Yes, for ethidium bromide staining, soak the gel in a 2 µg/mL solution of ethidium bromide in ultrapure water for 20 minutes. Destain by rinsing with three successive 10-minute rinses of ultrapure water. Visualize bands under UV light

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What should the running conditions be for the TBE gels (voltage, current, run time, etc.)?

Voltage: 200 V constant*
Approximate current at start: 10-18 mA/gel
Approximate current at end: 4-6 mA/gel
Run time: Approximately 30-90 minutes, dependent on gel percentage. The run is complete when the bromophenol blue (darker) tracking dye reaches the bottom of the gel.

* Voltages up to 250 V may be used to reduce run time.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

View all Novex™ TBE Gels, 20% FAQs

Citations & References (15)

Citations & References

Abstract

Prevalence and correlates of GB virus C infection in HIV-infected and HIV-uninfected pregnant women in Bangkok, Thailand.

Authors:Bhanich Supapol W, Remis RS, Raboud J, Millson M, Tappero J, Kaul R, Kulkarni P, McConnell MS, Mock PA, McNicholl JM, Roongpisuthipong A, Chotpitayasunondh T, Shaffer N, Butera S

Journal:J Med Virol

PubMed ID:21108337

'GB virus C (GBV-C) is an apathogenic virus that has been shown to inhibit HIV replication. This study examined the prevalence and correlates of GBV-C infection and clearance in three cohorts of pregnant women in Thailand. The study population consisted of 1,719 (1,387 HIV-infected and 332 HIV-uninfected) women from three ... More

An in vitro DNA double-strand break repair assay based on end-joining of defined duplex oligonucleotides.

Authors:Datta K, Purkayastha S, Neumann RD, Winters TA

Journal:Methods Mol Biol

PubMed ID:22941624

'DNA double-strand breaks (DSBs) are caused by endogenous cellular processes such as oxidative metabolism, or by exogenous events like exposure to ionizing radiation or other genotoxic agents. Repair of these DSBs is essential for the maintenance of cellular genomic integrity. In human cells, and cells of other higher eukaryotes, DSBs ... More

Smooth muscle phenotypic diversity is mediated through alterations in myocardin gene splicing.

Authors:Ilagan RM, Genheimer CW, Quinlan SF, Guthrie KI, Sangha N, Ramachandrannair S, Kelley RW, Presnell SC, Basu J, Ludlow JW

Journal:J Cell Physiol

PubMed ID:21792927

'Myocardin (MYOCD) is a smooth and cardiac muscle-specific transcriptional coactivator that is required for the proper expression of contraction-related genes. Through its function to transactivate effector genes, MYOCD plays an essential role in mediating the switch between contractile and non-contractile phenotypes, particularly in smooth muscle cells (SMC). There are at ... More

Genome wide full-length transcript analysis using 5' and 3' paired-end-tag next generation sequencing (RNA-PET).

Authors:Ruan X, Ruan Y

Journal:Methods Mol Biol

PubMed ID:22113299

'RNA-PET is a paired end tag (PET) sequencing method for full-length mRNA transcripts analysis using the next generation sequencer platforms such as Illumina GA and SOLiD. Unlike RNA-Seq method that sequences randomly sheared shotgun RNA short fragments, RNA-PET captures and sequences the 5'' and 3'' end tags of full-length cDNA ... More

Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition.

Authors:Adey A, Morrison HG, Asan Xun X, Kitzman JO, Turner EH, Stackhouse B, MacKenzie AP, Caruccio NC, Zhang X, Shendure J

Journal:Genome Biol

PubMed ID:21143862

We characterize and extend a highly efficient method for constructing shotgun fragment libraries in which transposase catalyzes in vitro DNA fragmentation and adaptor incorporation simultaneously. We apply this method to sequencing a human genome and find that coverage biases are comparable to those of conventional protocols. We also extend its ... More

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