This product will be unavailable for purchase after December 31, 2017. The price per box will be increasing on July 15, 2017. This product is being replaced by our new Novex Zymogram Plus gels. We recommend Cat. No. ZY00100BOX as an alternative. Learn about our New Zymogram Plus Protein gels. Please contact us with any questions.

Novex™ 4-16% Zymogram (Blue Casein) Protein Gels, 1.0 mm, 10-well

Catalog number:  EC6415BOX

Invitrogen™  Related applications: Protein Gel Electrophoresis

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Description

Novex® Zymogram Gels are excellent tools for detecting and characterizing proteases that utilize casein or gelatin as a substrate. Proteases are run on a gel containing one of these substrates under denaturing conditions and visualized as clear bands against a dark background following a simple renaturing, developing, and staining protocol. Zymogram gels are commonly used to detect matrix metalloproteinases.

Formulation: All Novex® Zymogram Gels are Tris-Glycine gels with a substrate (gelatin or casein) incorporated into the gel. They are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, highly purified water and casein or gelatin as the substrate. All have a 4% stacking gel.

Zymogram Gel Types: The 10% Zymogram (Gelatin) Gel is a 10% Tris-Glycine gel with 0.1% gelatin as the substrate. The 12% Novex® Zymogram (Casein) Gel is a 12% Tris-Glycine gel with 0.05% casein as the substrate. The 4-16% Novex® Zymogram (Blue Casein) Gel is a 4-16% Novex® Tris-Glycine gel with 0.1% casein as the substrate and a proprietary dye incorporated throughout the gel.

Sensitivity Level:
10% Zymogram Gelatin Gel: 5 x 10-6 units of collagenase
12% Zymogram Casein Gel: 7 x 10-4 units of trypsin
4-16% Zymogram Casein Gel: 1.5 x 10-3 units of trypsin

Recommended Buffers: Use Novex® Tris-Glycine SDS Sample Buffer and Novex® Tris-Glycine SDS Running Buffer with these gels. Novex® Zymogram Renaturing and Developing Buffers are also recommended.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): Mini Gel Tank, XCell SureLock™ Mini
Gel Percentage: 4-16 %
Gel Size: Mini (8 cm x 8 cm)
Gel Type: Zymogram Gel
Mode of Separation: Molecular Weight
Product Line: Novex™
Product Size: 10 gels (1 box)
Quantity: 10 gels
Sample Loading Volume: 25 µL
Separation Range: 10 to 220 kDa (Tris-Glycine Buffer)
Substrate: Casein
Substrate Type: Protease Substrate
System: Novex®
Thickness: 1.0 mm
Well Format: 1D Well
Wells: 10 wells
Shipping Condition: Wet Ice

Contents & storage

One box contains 10 gels. Store in refrigerator (2–8°C). Do not freeze. Shelf life is 8 weeks.

Documents

Manuals & protocols

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