High-density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit
High-density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit
Invitrogen™

High-density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit

The High-density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit is a highly reliable and sensitive assay designed for the quantitative measurementRead more
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Catalog number EEA012
Price (USD)/ 96 tests
618.80
Add to cart
Price (USD)/ 96 tests
618.80
Add to cart
High-density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit
Catalog numberEEA012
Price (USD)/ 96 tests
618.80
-
Add to cart

The High-density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit is a highly reliable and sensitive assay designed for the quantitative measurement of HDL-C in serum, plasma, culture supernatant, tissue, and cells. HDL-C is an anti-atherosclerotic lipoprotein that can transport cholesterol from extrahepatic tissues to the liver for metabolism and excretion of bile from the body. Its plasma level is negatively correlated with the risk of cardiovascular disease.

The assay is based on the reaction of HDL-C with enzyme reagents containing cholesterol esterase and cholesterol oxidase to produce hydrogen peroxide. The hydrogen peroxide is catalyzed by an oxidase in the presence of 4-aminoantipyrine (4-AA) and phenol (T-OOS) to form a red quinone compound. The colored substance has a maximum absorption peak at 546 nm. The HDL-C content in the sample can be calculated by measuring the OD value at 546 nm.

Key features
• Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits—across key analytes and targets within the most important metabolic pathways
• Consistent—strict quality control criteria to ensure reproducible and reliable results

Specifications
Assay Range0.065–3.8 mmol/L
Assay Sensitivity0.065 mmol/L
Detection MethodColorimetric
Sample TypeCell Culture Supernatant, Plasma, Serum, Tissue Homogenates, Whole Cells
For Use With (Equipment)Microplate Reader
Incubation Time10 min.
Interassay CV5
Intraassay CV3
Sample Volume2.5 μL
Shipping ConditionDry Ice, 2–8°C
Target Kit NamedHigh-density Lipoprotein Cholesterol (HDL-C)
Total Assay Time20 min.
Unit Size96 tests
Contents & Storage
Store at 2–8°C.