Thermo Scientific Pyrophosphatase, Inorganic catalyzes the hydrolysis of inorganic pyrophosphate into two orthophosphates. The enzyme requires a divalent metal cation with Mg2+
conferring the highest activity.Highlights
• Active in Thermo Scientific buffers for DNA polymerases
, RNA polymerases
, and Reverse TranscriptasesApplications
• High yield RNA synthesis by in vitro
• DNA polymerization reactions: preventing accumulation of pyrophosphate (see
References 3, 4)
• Removal of contaminant PPi
in reagents used for SNP genotyping by methods based on the detection of pyrophosphateNote
The enzyme can be diluted with the supplied storage (dilution) buffer. Use of this enzyme in certain applications may be covered by patents and may require a license.
For Research Use Only. Not for use in diagnostic procedures.