IL-1 alpha Human ELISA Kit

Catalog number:  EH2IL1A

Invitrogen™  Related applications: Protein Assays and Analysis

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The Thermo Scientific Human IL-1 alpha ELISA kit contains one precoated 96-well Strip Plate and sufficient reagents to assay IL-1α (human interleukin-1 alpha) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples. Each kit contains detection antibody, Streptavidin-HRP, buffers, diluents, TMB substrate and stop solutions, and sufficient IL-1α standard to generate a standard curve with replicates.

Features of the Thermo Scientific Human IL-1 alpha ELISA kit :

Target: natural and recombinant human interleukin-1 alpha (IL-1α)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 10.2 to 400 pg/mL
Sensitivity: < 2 pg/mL
Precision: intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Sample Types: cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with human IFNα, IFNγ, IL-1β, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFα, or mouse IL-1α. Human IL-1ra does not interfere with this ELISA.

These human IL-1 alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 alpha (IL-1α) in samples at 50 per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.
For Research Use Only. Not for use in diagnostic procedures.


Protein Family: Cytokines & Receptors
Assay Range: See product documentation
Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: IL-1A, IL1, IL1-ALPHA, IL1F1
Gene ID: 3552
Gene Name: interleukin 1 alpha
Gene Symbol: IL1A
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 tests
Protein Aliases: IL-1 alpha, Hematopoietin-1
Protein Name: Interleukin-1 alpha
Target (Kit Named): IL-1 alpha
Target Species (Named): Human
Target Species (Validated): Human
UniProt ID: P01583

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.


Manuals & protocols