CD25 Human ELISA Kit

Catalog number: EH2IL2R

Invitrogen™  Related applications: Protein Assays and Analysis

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Use the Thermo Scientific® Human IL-2R ELISA Kit to assay sIL-2R (human soluble interleukin-2 receptor; also called CD25) levels in human serum, plasma (with EDTA, heparin, sodium citrate), and cell culture supernatant samples. This is an alternate product for Cat. No. KHR0021.

This kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of sIL-2R levels in samples. The Thermo Scientific® Human IL-2R ELISA Kit also includes calibrated recombinant protein standard, TMB substrate solution, and stop solution. This complete kit contains one pre-coated 96-well strip plate and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

• Target: natural and recombinant human soluble interleukin-2 receptor (sIL-2R), also called CD25
• Assay range: 0 to 6500 U/mL*
• Sensitivity: <24 U/mL*
• Sample types: cell culture supernatant, serum, plasma (with EDTA, sodium citrate, heparin)
• Total assay time: 2.5 hours
• Sample size: 50 μL
• Specificity: no significant cross-reaction with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, GM-CSF, IFNγ, IFNα, TNFβ, or mouse IL-2

*Note: Standards in this ELISA are calibrated to an internal sIL-2R reference preparation of supernatant from a transfected cell line that secretes recombinant soluble IL-2R. We define 1000 units as the amount of sIL-2R present in 1 mL of an internal reference preparation. Units/mL can be converted to sIL-2R pg/mL using the following formula: (sIL-2R pg/mL) = (sIL-2R U/mL) x (1/0.113).
For Research Use Only. Not for use in diagnostic procedures.


Protein Family: Cytokines & Receptors
Assay Range: See product documentation
Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: CD25, IDDM10, IL2R, IMD41, TCGFR, p55
Gene ID: 3559
Gene Name: interleukin 2 receptor subunit alpha
Gene Symbol: IL2RA
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 tests
Protein Aliases: IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55
Protein Name: Interleukin-2 receptor subunit alpha
Target (Kit Named): CD25
Target Species (Named): Human
Target Species (Validated): Human
UniProt ID: P01589

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.


Manuals & protocols