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CD25 Human ELISA Kit

Catalog number: EH2IL2R5

Invitrogen™  Related applications: Protein Assays and Analysis

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The Thermo Scientific Human IL-2R ELISA Kit is used to measure s-IL-2R (human soluble interleukin-2 receptor) levels in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatants. Each kit contains five anti-Human sIL-2R capture antibody precoated 96-well Strip Plates and sufficient detection antibody, conjugate, buffers, diluent, TMB and stop buffers, as well as lyophilized sIL-2R standard to analyze several samples.

Features of the Thermo Scientific Human IL-2R ELISA Kit:

Target: natural and recombinant human soluble interleukin-2 receptor (sIL-2R); also called CD25
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 0 to 6500 U/mL
Sensitivity: < 24 U/mL
Precision: intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 2.5 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, GM-CSF, IFNγ, IFNα, TNFβ, or mouse IL-2.

Note: Standards in this ELISA are calibrated to an internal sIL-2R reference preparation of supernatant from a transfected cell line that secretes recombinant soluble IL-2R. We define 1000 units as the amount of sIL-2R present in 1 mL of an internal reference preparation. Units/mL can be converted to sIL-2R pg/mL using the following formula: (sIL-2R pg/mL) = (sIL-2R U/mL) x (1/0.113).

This human IL-2 receptor ELISA kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of soluble interleukin-2 receptor (sIL-2R, also called CD25) in samples at 50 µL per well. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kit contains pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.
For Research Use Only. Not for use in diagnostic procedures.


Protein Family: Cytokines & Receptors
Assay Range: See product documentation
Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: CD25, IDDM10, IL2R, IMD41, TCGFR, p55
Gene ID: 3559
Gene Name: interleukin 2 receptor subunit alpha
Gene Symbol: IL2RA
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 5 x 96 tests
Protein Aliases: IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55
Protein Name: Interleukin-2 receptor subunit alpha
Target (Kit Named): CD25
Target Species (Named): Human
Target Species (Validated): Human
UniProt ID: P01589

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.


Manuals & protocols