This product is only available through your local Thermo Scientific supplier. Learn where to buy >

CCL3 Human ELISA Kit

Catalog number: EH2MIP1A2

Invitrogen™  Related applications: Protein Assays and Analysis

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
{{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

Assay MIP-1α (human macrophage inflammatory protein-1 alpha) levels in human serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples with our Thermo Scientific Human MIP-1 alpha ELISA Kits.

Features of the Human MIP-1 alpha ELISA Kit:

Target: natural and recombinant human MIP-1α (macrophage inflammatory protein-1 alpha)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450 nm minus absorbance at 550nm
Assay Range: 25.6 to 1000 pg/mL
Sensitivity: < 2.5 pg/mL
Precision: intra-assay CV < 10%; inter-assay CV < 10%
Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with human MIP-1β, MIP-1δ, MIP-3α, MIP-3β mouse MIP-1α, MIP-1β, MIP-1γ, MIP-3β or rat MIP-3α, MIP-I and MIP-II.

These human MIP-1 alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of macrophage inflammatory protein-1 alpha (MIP-1α) in samples at 50 µL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: Chemokines & Receptors
Assay Range: See product documentation
Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: G0S19-1, LD78ALPHA, MIP-1-alpha, MIP1A, SCYA3
Gene ID: 6348
Gene Name: C-C motif chemokine ligand 3
Gene Symbol: CCL3
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 2 x 96 tests
Protein Name: C-C motif chemokine 3
Target (Kit Named): CCL3
Target Species (Named): Human
Target Species (Validated): Human
UniProt ID: P10147

Contents & storage

Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols