MIP-1 alpha ELISA Kit, Human

Catalog number: EH2MIP1A2

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Assay MIP-1α (human macrophage inflammatory protein-1 alpha) levels in human serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples with our Thermo Scientific Human MIP-1 alpha ELISA Kits.

Features of the Human MIP-1 alpha ELISA Kit:

Target: natural and recombinant human MIP-1α (macrophage inflammatory protein-1 alpha)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450 nm minus absorbance at 550nm
Assay Range: 25.6 to 1000 pg/mL
Sensitivity: < 2.5 pg/mL
Precision: intra-assay CV < 10%; inter-assay CV < 10%
Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with human MIP-1β, MIP-1δ, MIP-3α, MIP-3β mouse MIP-1α, MIP-1β, MIP-1γ, MIP-3β or rat MIP-3α, MIP-I and MIP-II.

These human MIP-1 alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of macrophage inflammatory protein-1 alpha (MIP-1α) in samples at 50 µL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
For Research Use Only. Not for use in diagnostic procedures.


Species: Human
Product Size: 192 assays
Protein Family: Chemokines & Receptors
Detection Method: Colorimetric
Research Category: Immunology

Contents & storage

Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.


Manuals & protocols