IL-4 ELISA Kit, Human

Catalog number: EH3IL4

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

The Thermo Scientific Human IL-4 ELISA Kit is used to measure IL-4 (human interleukin-4) levels in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatants. Each kit contains one anti-Human sIL-2R capture antibody precoated 96-well Strip Plate and sufficient detection antibody, conjugate, buffers, diluent, TMB and stop buffers, as well as lyophilized sIL-2R standard to analyze several samples.

Features of the Thermo Scientific Human IL-4 ELISA Kit:

Target: natural and recombinant human interleukin-4 (IL-4; also called BSF-1 or BCGF-1)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 10.24 to 400 pg/mL
Sensitivity: ≤ 2 pg/mL
Precision: intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Sample Types: cell culture supernatant, serum and plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3.5 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with human IL-2, IL-6, IL-10, IFNγ, GM-CSF or mouse IL-4.

Includes:
Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

The human IL-4 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-4 (IL-4), also known as B-cell stimulatory factor 1 (BSF-1, BSF1) or B-cell growth factor 1 (BCGF-1, BCGF1), in samples at 50 µL per well. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

The Thermo Scientific Human IL-4 ELISA Kit measures human interleukin-4 (hIL-4) in serum, plasma and cell culture supernatants. Produced by activated T-lymphocytes, hIL-4 (15-19kDa) is a glycosylated cytokine involved in the processes of hematopoiesis, allergic inflammation and antibody production. This ELISA kit uses a matched pair of monoclonal antibodies to capture and detect hIL-4 on 96-well plates. The detecting antibody is conjugated to horseradish peroxidase (HRP), which reacts with the TMB substrate to form a colored product that is linear over the assay dynamic range and directly proportional to the amount of hIL-4 present in the sample.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Species: Human
Product Size: 96 assays
Protein Family: Cytokines & Receptors
Detection Method: Colorimetric
Research Category: Immunology

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols