This product is going to be discontinued. Please see alternate Cat. No. KHC3011.

TNF alpha Human ELISA Kit

Catalog number: EH3TNFA

Invitrogen™  Related applications: Protein Assays and Analysis

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Assay TNFα (human tumor necrosis factor alpha) levels in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Human TNF alpha ELISA Kit.

Features of the Human TNF alpha ELISA Kit:

Target: natural and recombinant human TNFα (human tumor necrosis factor alpha)
Format: colorimetric ELISA (TMB substrate) in a clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 15.6 to 1000 pg/mL
Sensitivity: < 2 pg/mL
Precision: dependent upon sample type and level (see instructions)
Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3.5 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12, IL-12p40, IL-13, IL-15, IL-16, IL-17, IL-18, TNFβ, Eotaxin, RANTES, GROα, GROβ, MCP-1, MCP-2, MCP-3, MCP-4, VEGF, GCSF, GMCSF, MIP-1α, MIP-1β, TGFβ, IFNγ, IFNα mouse TNFα rat TNFα and bovine TNFα. TNFα Receptor types 1 and 2 tested at 40 mg/mL did not interfere in this assay. Recombinant pig TNFα cross-reacted at 100%.

This human TNF alpha ELISA kits uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50 to 100 µL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kit contains a precoated 96-well strip plate and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

» Compare ELISA Kit formats »

TNFα, also called cachectin, macrophage cytotoxin, necrosin, etc., is produced primarily by macrophages in response to bacteria, viruses, mycoplasma, immune complexes, other cytokines, tumor cells, complement and phorbol esters among others. It is a 157 amino acid long polypeptide with an apparent MW of 17 kDa under denaturing conditions but exists as a 45-55 kDa trimer naturally.

In vivo, TNFα has anti-tumor activities against some solid tumors, induces tumorigenesis, metastisis; acts in immunomodulation; induces septic shock, fever; plays a role in multiple sclerosis; and is involved in autoimmunity and infectious disease. TNFα alone and in combination with other cytokines and chemical agents has been used as an anti-tumor agent. Patients with melanoma and other solid tumors, administered high doses of TNFα, have realized a cure rate of up to 90% albeit with some major and minor side effects.
For Research Use Only. Not for use in diagnostic procedures.


Protein Family: Cytokines & Receptors
Assay Range: See product documentation
Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: DIF-alpha, TNFA, TNFSF2, TNLG1F, TNF
Gene ID: 7124
Gene Name: tumor necrosis factor
Gene Symbol: TNF
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 tests
Protein Name: tumor necrosis factor
Target (Kit Named): TNF alpha
Target Species (Named): Human
Target Species (Validated): Human
UniProt ID: P01375

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.


Manuals & protocols