EGF ELISA Kit, Human

Catalog number: EHEGF

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

The Thermo Scientific Human EGF ELISA Kit contains sufficient reagents and buffers to assay EGF (epidermal growth factor) levels in human serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples. Each kit is sufficient for 96 microplate wells

Features of the Thermo Scientific Human EGF ELISA Kit:

Target: natural and recombinant human epidermal growth factor (EGF); also known as urogastrone (URG), hypomagnesemia type 4 (HOMG4)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 7.8 to 500 pg/mL
Sensitivity: ≤ 5 pg/mL
Reproducibility: intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Sample Types: cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3.5 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with human HB-EGF (Heparin-binding EGF), mouse EGF, rat EGF and human IL-2, IL-4, IL-6, IL-8, TNFα TGFβ, GM-CSF

Note: Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.

These human EGF ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of epidermal growth factor (EGF, also called URG, HOMG4) in samples at 50 to 100 µL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched EGF antibody pairs and EGF standard only. All components undergo quality control testing to minimize lot-to-lot variability.

Includes:
Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a "MiniKit") contains only matched antibodies and standard (no plates or detection reagents).

» Compare ELISA Kit formats »
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Colorimetric
Product Size: 96 assays
Protein Family: Growth Factors & Receptors
Research Category: Immunology
Species: Human

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols