CXCL11 Human ELISA Kit

Catalog number: EHITAC

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

Assay I-TAC (human interferon-inducible T-cell alpha chemoattractant) levels in human serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples with our Thermo Scientific Human I-TAC ELISA Kits.

Features of the Human ITAC ELISA Kit:

Target: natural and recombinant human I-TAC (human interferon-inducible T-cell alpha chemoattractant, also called CXCL11, IP9)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450 nm minus absorbance at 550nm
Assay Range: 41.0 to 4000 pg/mL
Sensitivity: < 5 pg/mL
Precision: intra-assay CV < 10%; inter-assay CV < 10%
Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3.5 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with human MIG/CXCL9, IP-10/CXCL10, IL-8, IFNα, or mouse ITAC/CXCL11.

These human I-TAC ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of I-TAC (also known as CXCL11 or IP9) in samples at 50 µL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Name: C-X-C motif chemokine ligand 11
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 tests
Protein Name: C-X-C motif chemokine 11
Target (Kit Named): CXCL11
Target Species (Named): Human
Target Species (Validated): Human
UniProt ID: O14625

Contents & storage

For maximum stability, store at -20°C.

Documents

Manuals & protocols