CpG Methyltransferase (M.SssI)

Thermo Scientific M.SssI methylates the C5 position on the base moiety of all cytosine nucleotides contained in unmethylated or hemimethylated double stranded DNA in a 5’-CpG-3’ context.
The enzyme is specifically formulated for fast reaction times without compromising the reaction efficiency. The enzyme completes modification of all CpGs in 15 minutes at 37°C. In addition, the enzyme has been specifically validated for use with genomic DNA – the primary substrate in epigenetic studies.

M.SssI is supplied with an optimized 10X reaction buffer and 50X S-adenosylmethionine (SAM) as a cofactor.

Highlights

• High efficiency – complete in vitro methylation of all CpGs in non-methylated and hemi-methylated DNA
• Fast – reaction is completed in 15 minutes at 37°C
• Stable – at -20°C for 2 years

Applications

• Epigenetics studies
• in vitro methylation of DNA for methylation analysis
• Inhibition of endonucleases with overlapping CpG sequence recognition.
• [3H]-labeling of DNA

The enzyme methylates the C5 position on the base moiety of all cytosine nucleotides contained in unmethylated or hemimethylated double stranded DNA in a 5’-CpG-3’ context.

Thermo Scientific M.SssI is specifically formulated for fast reaction times without compromising the reaction efficiency. The enzyme completes modification of all CpGs in 15 minutes at 37°C. In addition, the enzyme has been specifically validated for use with genomic DNA – the primary substrate in epigenetic studies.

M.SssI is supplied with an optimized 10X reaction buffer and 50X S-adenosylmethionine (SAM) as a cofactor.

Highlights

High efficiency – complete in vitro methylation of all CpGs in non-methylated and hemi-methylated DNA
Fast – reaction is completed in 15 minutes at 37°C
Stable – at -20°C for 2 years

Applications

• Epigenetics studies
• in vitro methylation of DNA for methylation analysis
• Inhibition of endonucleases with overlapping CpG sequence recognition.
• [3H]-labeling of DNA