IL-1 beta ELISA Kit, Mouse

Catalog number: EM2IL1B5

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Assay IL-1β (mouse interleukin-1 beta) levels in mouse serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Mouse IL-1 beta ELISA Kit.

Features of the Mouse IL-1 beta ELISA Kit:

Target: natural and recombinant mouse interleukin-1 beta (IL-1β)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 31.2 to 2000 pg/mL
Sensitivity: < 3 pg/mL
Precision: intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 4 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with mouse IL-1α, IL-10, IL-2, IL-4, IL-6, TNFα, GM-CSF; human IL-1β, IL-8, IL-1α or bovine IL-1β. This ELISA does cross-react with rat IL-1β (12%).

This mouse IL-1 beta ELISA kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 beta (IL-1β) in samples at 50 µL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kit contains pre-coated 96-well strip plates and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Complete kit contains pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.
For Research Use Only. Not for use in diagnostic procedures.


Species: Mouse
Product Size: 480 assays
Protein Family: Cytokines & Receptors
Detection Method: Colorimetric
Research Category: Immunology

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.


Manuals & protocols