ICAM-1 (Soluble) Mouse ELISA Kit

Catalog number: EMICAM12

Invitrogen™  Related applications: Protein Assays and Analysis

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Assay sICAM-1 (mouse soluble ICAM-1, also called CD54) levels in mouse serum, plasma (EDTA) and culture supernatant samples with our Thermo Scientific Mouse Soluble ICAM (CD54) ELISA Kit.

Features of the Mouse Soluble ICAM (CD54) ELISA Kit:

Target: mouse soluble ICAM-1 (intercellular adhesion molecule 1, also called CD54)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plates
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 25.6 to 1000 ng/mL
Sensitivity: < 5 ng/mL
Precision: intra-assay CV < 10%; inter-assay CV < 10%
Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3.5 hours
Sample Size: 50 µL of 100-fold diluted samples per well
Specificity: no significant cross-reaction with rat or human sICAM-1

This mouse sICAM-1 ELISA kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of soluble ICAM-1 (intercellular adhesion molecule 1, also called CD54) in samples at 50 µL per well. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kit contains pre-coated 96-well strip plates and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
For Research Use Only. Not for use in diagnostic procedures.


Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: CD54, Icam-1, Ly-47, MALA-2
Gene ID: 15894
Gene Name: intercellular adhesion molecule 1
Gene Symbol: ICAM1
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 2 x 96 tests
Protein Aliases: ICAM-1, MALA-2, MyD10
Protein Name: intercellular adhesion molecule 1
Target (Kit Named): ICAM-1
Target Species (Named): Mouse
Target Species (Validated): Mouse
Target Sub-Specificity: Soluble
UniProt ID: P13597

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.


Manuals & protocols