IL-1 alpha Mouse ELISA Kit

Catalog number: EMIL1A5

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

Assay IL-1α (mouse interleukin-1 alpha) levels in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Mouse IL-1 alpha ELISA Kit.

Features of the Mouse IL-1 alpha ELISA Kit:

Target: natural and recombinant mouse interleukin-1 alpha (IL-1α)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 15.6 to 1000 pg/mL
Sensitivity: < 6 pg/mL
Precision: intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with human IL-1α, human IL-1β, or mouse IL-1β, IL-3, IL-4, IL-5, IL-7, IL-10, TNFα, IFNγ, or GM-CSF.

Theis mouse IL-1 alpha ELISA kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 alpha (IL-1α) in samples at 50 per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kit contains precoated 96-well strip plates and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Includes:
Complete kit contains pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: Il-1a
Gene ID: 16175
Gene Name: interleukin 1 alpha
Gene Symbol: IL1A
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 5 x 96 tests
Protein Aliases: IL-1 alpha
Protein Name: Interleukin-1 alpha
Target (Kit Named): IL-1 alpha
Target Species (Named): Mouse
Target Species (Validated): Mouse
UniProt ID: P01582

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols